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Literature summary extracted from
- Porcine cholesterol esterase, a multiform enzyme (1983), Biochim. Biophys. Acta, 749, 32-41.
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.1.13 | extracellular | - |
Sus scrofa | - |
- |
| 3.1.1.13 | intracellular | - |
Sus scrofa | 5622 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.13 | 45000 | - |
gel filtration (HPLC separation) | Sus scrofa |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.13 | cholesteryl esters + H2O | Sus scrofa | - |
cholesterol + fatty acid | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.13 | Sus scrofa | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.1.1.13 | side-chain modification | glycoprotein, glucosamine, no galactosamine, neutral sugars | Sus scrofa |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.1.13 | to homogeneity by digitonin solubilization, chromatography steps, isoelectric focusing, isocratic HPLC | Sus scrofa |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.1.13 | pancreas | - |
Sus scrofa | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.13 | 0.11 | - |
for cholesteryl oleate in monomeric enzyme | Sus scrofa |
| 3.1.1.13 | 0.19 | - |
for cholesteryl oleate in dimeric enzyme | Sus scrofa |
| 3.1.1.13 | 16.43 | - |
for p-nitrophenyl acetate in monomeric enzyme | Sus scrofa |
| 3.1.1.13 | 25.93 | - |
for p-nitrophenyl acetate in dimeric enzyme | Sus scrofa |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.13 | cholesteryl esters + H2O | - |
Sus scrofa | cholesterol + fatty acid | - |
? | |
| 3.1.1.13 | cholesteryl oleate + H2O | - |
Sus scrofa | cholesterol + oleic acid | - |
? | |
| 3.1.1.13 | p-nitrophenyl acetate + H2O | - |
Sus scrofa | p-nitrophenol + acetic acid | - |
ir | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.1.13 | dimer | 2 * 45000 gel filtration (HPLC separation) | Sus scrofa |
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