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Literature summary extracted from

  • Sando, G.N.; Rosenbaum, L.M.
    Human lysosomal acid lipase/cholesteryl ester hydrolase. Purification and properties of the form secreted by fibroblasts in microcarrier culture (1985), J. Biol. Chem., 260, 15186-15193.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.13 ammonium chloride 25% inhibition Homo sapiens
3.1.1.13 Ca2+ 50% inhibition at 5 mM Homo sapiens
3.1.1.13 cardiolipin inhibitory if included as aqueous sonicated dispersions at 2.5 mg/ml Homo sapiens
3.1.1.13 Chloropromazine 50% inhibition at 0.05 mM Homo sapiens
3.1.1.13 dodecylboronic acid 50% inhibition at 1 mM Homo sapiens
3.1.1.13 KCl 25% inhibition at 0.15 M Homo sapiens
3.1.1.13 MgSO4 abolishes activity at 0.15 M Homo sapiens
3.1.1.13 N-ethylmaleimide 50% inhibition at 50 mM in 10 min Homo sapiens
3.1.1.13 NaCl 25% inhibition at 0.15 M Homo sapiens
3.1.1.13 NaF abolishes activity at 0.15 M Homo sapiens
3.1.1.13 p-hydroxymercuribenzoate 50% inhibition at 0.2-0.4 mM Homo sapiens
3.1.1.13 phenylboronic acid 50% inhibition at 10 mM Homo sapiens
3.1.1.13 phosphatidylinositol inhibitory if included as aqueous sonicated dispersions at 2.5 mg/ml Homo sapiens
3.1.1.13 phosphatidylserine inhibitory if included as aqueous sonicated dispersions at 2.5 mg/ml Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.13 cholesteryl esters + H2O Homo sapiens
-
 cholesterol + fatty acid
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.13 Homo sapiens
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
3.1.1.13 thiols required for stabilizing activity Homo sapiens

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.13 side-chain modification glycoprotein Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.13 fibroblast
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.13 0.15
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.13 cholesteryl esters + H2O
-
 Homo sapiens cholesterol + fatty acid
-
 ?
3.1.1.13 cholesteryl oleate + H2O
-
 Homo sapiens cholesterol + oleic acid
-
 ?
3.1.1.13 triolein + H2O
-
 Homo sapiens dioleoylglycerol + oleic acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.13 acid cholesterol esterase
-
 Homo sapiens
3.1.1.13 acid lipase
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.13 4
-
-
 Homo sapiens
3.1.1.13 5 6 in presence of 0.05-0.5 M NaCl, bovine serum albumin or ovalbumin Homo sapiens
3.1.1.13 6
-
 for fatty acyl esters with 9 or less carbons Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.1.13 0.05
-
 Chloropromazine
-
 Homo sapiens
3.1.1.13 1
-
 dodecylboronic acid
-
 Homo sapiens
3.1.1.13 10
-
 phenylboronic acid
-
 Homo sapiens