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Literature summary extracted from
- Testicular temperature-labile cholesteryl ester hydrolase (1993), J. Biol. Chem., 268, 8158-8163.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.1.1.13 | ammonium sulfate precipitation increases stability | Rattus sp. |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.13 | Alkaline phosphatase | 65% inhibition | Rattus sp. | |
| 3.1.1.13 | Cd2+ | very potent inhibitor | Rattus sp. |  |
| 3.1.1.13 | cholesterol | 80-100% inhibition at 0.01-0.15 mM | Rattus sp. | |
| 3.1.1.13 | Cu2+ | at very low concentrations | Rattus sp. | |
| 3.1.1.13 | oleate | 25-40% inhibition at 0.002-0.003 mM | Rattus sp. | |
| 3.1.1.13 | Zn2+ | at very low concentrations | Rattus sp. | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.1.13 | cytosol | - |
Rattus sp. | 5829 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.13 | 66000 | - |
SDS-PAGE | Rattus sp. |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.13 | cholesteryl esters + H2O | Rattus sp. | involvement in inhibition of spermatogenesis | cholesterol + fatty acid | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.13 | Rattus sp. | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.1.13 | 2000fold to homogeneity by chromatography techniques | Rattus sp. |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.1.13 | testis | exclusively localized | Rattus sp. | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.13 | 1 | - |
- |
Rattus sp. |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.13 | cholesteryl esters + H2O | involvement in inhibition of spermatogenesis | Rattus sp. | cholesterol + fatty acid | - |
? | |
| 3.1.1.13 | cholesteryl oleate + H2O | - |
Rattus sp. | cholesterol + oleic acid | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.1.13 | monomer | 1 * 66000, SDS-PAGE after isoelectric focusing chromatography | Rattus sp. |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.1.13 | CEH | - |
Rattus sp. |
| 3.1.1.13 | testicular temperature-labile cholesteryl ester hydrolase | - |
Rattus sp. |
| 3.1.1.13 | TLCEH | - |
Rattus sp. |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.13 | 37 | - |
inactivation by evaluation from 32°C to 37°C | Rattus sp. |
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