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Literature summary extracted from

  • Storey, K.B.
    Tissue-specific alanopine dehydrogenase and strombine dehydrogenase from the sea mouse, Aphrodite aculeata (polychaeta) (1983), J. Exp. Zool., 225, 369-378.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.17 acetate
-
Aphrodita aculeata
1.5.1.17 ATP
-
Aphrodita aculeata
1.5.1.17 D-lactate strong Aphrodita aculeata
1.5.1.17 L-lactate strong Aphrodita aculeata
1.5.1.17 meso-Alanopine strong Aphrodita aculeata
1.5.1.17 NAD+ product inhibition Aphrodita aculeata
1.5.1.17 pyruvate substrate inhibition Aphrodita aculeata
1.5.1.22 D-strombine
-
Aphrodita aculeata
1.5.1.22 iminodiacetic acid
-
Aphrodita aculeata
1.5.1.22 meso-Alanopine
-
Aphrodita aculeata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.17 0.2
-
pyruvate
-
Aphrodita aculeata
1.5.1.17 0.27
-
pyruvate
-
Aphrodita aculeata
1.5.1.17 0.75
-
NAD+
-
Aphrodita aculeata
1.5.1.17 0.9
-
oxaloacetate
-
Aphrodita aculeata
1.5.1.17 1.68
-
meso-Alanopine
-
Aphrodita aculeata
1.5.1.17 2.3
-
Hydroxypyruvate
-
Aphrodita aculeata
1.5.1.17 2.35
-
2-oxobutanoate
-
Aphrodita aculeata
1.5.1.17 2.75
-
L-Ala
-
Aphrodita aculeata
1.5.1.17 9
-
NADH L-2-aminobutanoate Aphrodita aculeata
1.5.1.17 9.1
-
2-oxopentanoate
-
Aphrodita aculeata
1.5.1.17 12
-
L-Cys
-
Aphrodita aculeata
1.5.1.17 12.5
-
L-Ala
-
Aphrodita aculeata
1.5.1.17 25.5
-
L-Thr
-
Aphrodita aculeata
1.5.1.17 48
-
L-Ser
-
Aphrodita aculeata
1.5.1.17 70.5
-
L-Val
-
Aphrodita aculeata
1.5.1.17 285
-
Gly
-
Aphrodita aculeata
1.5.1.17 430
-
Gly
-
Aphrodita aculeata
1.5.1.22 0.47
-
pyruvate
-
Aphrodita aculeata
1.5.1.22 1.7
-
oxaloacetate
-
Aphrodita aculeata
1.5.1.22 3.55
-
2-oxobutanoate
-
Aphrodita aculeata
1.5.1.22 8.5
-
Hydroxypyruvate
-
Aphrodita aculeata
1.5.1.22 23.8
-
2-oxopentanoate
-
Aphrodita aculeata
1.5.1.22 28
-
L-Cys
-
Aphrodita aculeata
1.5.1.22 32.5
-
glyoxylate
-
Aphrodita aculeata
1.5.1.22 36.5
-
Gly
-
Aphrodita aculeata
1.5.1.22 48
-
L-Thr
-
Aphrodita aculeata
1.5.1.22 170
-
L-Ala
-
Aphrodita aculeata
1.5.1.22 310
-
L-Val
-
Aphrodita aculeata
1.5.1.22 360
-
L-Ser
-
Aphrodita aculeata
1.5.1.22 457
-
L-2-aminobutanoate
-
Aphrodita aculeata

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.5.1.17 cytoplasm
-
Aphrodita aculeata 5737
-
1.5.1.17 cytosol
-
Aphrodita aculeata 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.17 44000
-
gel filtration Aphrodita aculeata
1.5.1.22 44000
-
gel filtration Aphrodita aculeata

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.17 Aphrodita aculeata
-
-
-
1.5.1.22 Aphrodita aculeata
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.17 partial Aphrodita aculeata

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.17 elytron
-
Aphrodita aculeata
-
1.5.1.17 muscle longitudinal Aphrodita aculeata
-
1.5.1.17 nerve
-
Aphrodita aculeata
-
1.5.1.22 intestine
-
Aphrodita aculeata
-
1.5.1.22 pharynx
-
Aphrodita aculeata
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.17 90
-
-
Aphrodita aculeata
1.5.1.22 204
-
-
Aphrodita aculeata

Storage Stability

EC Number Storage Stability Organism
1.5.1.17 4°C, stable for at least 1 week Aphrodita aculeata
1.5.1.22 4°C, stable for at least 1 week Aphrodita aculeata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.17 Gly + pyruvate + NADH r Aphrodita aculeata Strombine + NAD+ + H2O
-
?
1.5.1.17 Gly + pyruvate + NADH at 161.5% of the activity with L-Ala Aphrodita aculeata Strombine + NAD+ + H2O
-
?
1.5.1.17 L-2-Aminobutyrate + pyruvate + NADH at 60% of the activity with L-Ala Aphrodita aculeata ?
-
?
1.5.1.17 L-Ala + 2-oxobutanoate + NADH at 21% of the activity with pyruvate Aphrodita aculeata ?
-
?
1.5.1.17 L-Ala + 2-oxopentanoate + NADH at 25.6% of the activity with pyruvate Aphrodita aculeata ?
-
?
1.5.1.17 L-Ala + hydroxypyruvate + NADH at 12.8% of the activity with pyruvate Aphrodita aculeata ?
-
?
1.5.1.17 L-Ala + oxaloacetate + NADH at 106.6% of the activity with pyruvate Aphrodita aculeata ?
-
?
1.5.1.17 L-Ala + pyruvate + NADH r Aphrodita aculeata 2,2'-Iminodipropanoate + NAD+ + H2O
-
?
1.5.1.17 L-Cys + pyruvate + NADH at 130.8% of the activity with L-Ala Aphrodita aculeata ?
-
?
1.5.1.17 L-Ser + pyruvate + NADH at 150.8% of the activity with L-Ala Aphrodita aculeata ?
-
?
1.5.1.17 L-Thr + pyruvate + NADH as effective as L-Ala Aphrodita aculeata ?
-
?
1.5.1.17 L-Val + pyruvate + NADH at 71.5% of the activity with L-Ala Aphrodita aculeata ?
-
?
1.5.1.17 meso-alanopine + NAD+ + H2O
-
Aphrodita aculeata ?
-
r
1.5.1.22 Gly + 2-oxobutanoate + NADH at 24.8% of the activity with pyruvate Aphrodita aculeata ?
-
?
1.5.1.22 Gly + 2-oxopentanoate + NADH 19.6% of the activity with pyruvate Aphrodita aculeata ?
-
?
1.5.1.22 Gly + glyoxylate + NADH 23.6% of the activity with pyruvate Aphrodita aculeata ?
-
?
1.5.1.22 Gly + hydroxypyruvate + NADH 29.5% of the activity with pyruvate Aphrodita aculeata ?
-
?
1.5.1.22 Gly + oxaloacetate + NADH 106% of the activity with pyruvate Aphrodita aculeata ?
-
?
1.5.1.22 Gly + pyruvate + NADH
-
Aphrodita aculeata D-Strombine + NAD+ + H2O
-
?
1.5.1.22 L-2-Aminobutanoate + pyruvate + NADH 68.3% of the activity with Gly Aphrodita aculeata ?
-
?
1.5.1.22 L-Ala + pyruvate + NADH 101% of the activity with Gly Aphrodita aculeata meso-Alanopine + NAD+ + H2O
-
?
1.5.1.22 L-Cys + pyruvate + NADH 19.9% of the activity with Gly Aphrodita aculeata ?
-
?
1.5.1.22 L-Ser + pyruvate + NADH 88.5% of the activity with Gly Aphrodita aculeata ?
-
?
1.5.1.22 L-Thr + pyruvate + NADH 9.3% of the activity with Gly Aphrodita aculeata ?
-
?
1.5.1.22 L-Val + pyruvate + NADH 11.2% of the activity with Gly Aphrodita aculeata ?
-
?

Subunits

EC Number Subunits Comment Organism
1.5.1.22 monomer
-
Aphrodita aculeata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.17 6.5 7 with L-Ala and pyruvate as substrates Aphrodita aculeata
1.5.1.17 9
-
with meso-alanopine as substrate Aphrodita aculeata
1.5.1.22 6 6.5 with Gly and pyruvate as substrates Aphrodita aculeata
1.5.1.22 9
-
-
Aphrodita aculeata

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.17 NAD+
-
Aphrodita aculeata
1.5.1.17 NADH
-
Aphrodita aculeata
1.5.1.22 NAD+
-
Aphrodita aculeata
1.5.1.22 NADH
-
Aphrodita aculeata