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Literature summary extracted from

  • Fields, J.H.A.; Hochachka, P.W.
    Purification and properties of alanopine dehydrogenase from the adductur muscle of the oyster, Crassostrea gigas (mollusca, bivalvia) (1981), Eur. J. Biochem., 114, 615-621.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.17 2-oxoglutarate uncompetitive with respect to Ala and pyruvate Crassostrea gigas
1.5.1.17 ADP competitive with respect to NADH Crassostrea gigas
1.5.1.17 AMP competitive with respect to NADH Crassostrea gigas
1.5.1.17 ATP competitive with respect to NADH Crassostrea gigas
1.5.1.17 NAD+ competitive with respect to NADH Crassostrea gigas
1.5.1.17 oxamate
-
 Crassostrea gigas
1.5.1.17 succinate
-
 Crassostrea gigas

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.17 0.01
-
 NADH
-
 Crassostrea gigas
1.5.1.17 0.45
-
 pyruvate
-
 Crassostrea gigas
1.5.1.17 3.45
-
 2-oxobutanoate
-
 Crassostrea gigas
1.5.1.17 3.49
-
 glyoxylate
-
 Crassostrea gigas
1.5.1.17 6.48
-
 Hydroxypyruvate
-
 Crassostrea gigas
1.5.1.17 16
-
 2-oxovalerate
-
 Crassostrea gigas
1.5.1.17 96
-
 Gly
-
 Crassostrea gigas
1.5.1.17 98
-
 L-Ala
-
 Crassostrea gigas
1.5.1.17 150
-
 L-2-aminobutyrate
-
 Crassostrea gigas
1.5.1.17 680
-
 Ser
-
 Crassostrea gigas

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.17 46500
-
 1 * 46500, SDS-PAGE Crassostrea gigas
1.5.1.17 47200
-
 gel filtration Crassostrea gigas

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.17 Crassostrea gigas
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.17
-
 Crassostrea gigas

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.17 adductor
-
 Crassostrea gigas
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.17 691
-
-
 Crassostrea gigas

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.17 Gly + pyruvate + NADH
-
 Crassostrea gigas Strombine + NAD+ + H2O
-
 ?
1.5.1.17 L-2-Aminobutyrate + pyruvate + NADH
-
 Crassostrea gigas ?
-
 ?
1.5.1.17 L-2-aminobutyrate + pyruvate + NADH + H+
-
 Crassostrea gigas ?
-
 r
1.5.1.17 L-Ala + 2-oxobutanoate + NADH at 68% of the activity with pyruvate Crassostrea gigas ?
-
 ?
1.5.1.17 L-Ala + 2-oxopentanoate + NADH at 58% of the activity with pyruvate Crassostrea gigas ?
-
 ?
1.5.1.17 L-Ala + glyoxylate + NADH at 10.5% of the activity with pyruvate Crassostrea gigas ?
-
 ?
1.5.1.17 L-Ala + hydroxypyruvate + NADH at 3.3% of the activity with pyruvate Crassostrea gigas ?
-
 ?
1.5.1.17 L-Ala + pyruvate + NADH
-
 Crassostrea gigas 2,2'-Iminodipropanoate + NAD+ + H2O
-
 ?
1.5.1.17 L-Ser + pyruvate + NADH
-
 Crassostrea gigas ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.17 monomer 1 * 46500, SDS-PAGE Crassostrea gigas

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.17 NAD+
-
 Crassostrea gigas
1.5.1.17 NADH
-
 Crassostrea gigas