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Literature summary extracted from

  • Krell, K.; Eisenberg, M.A.
    The purification and properties of dethiobiotin synthetase (1970), J. Biol. Chem., 245, 6558-6566.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.3.3 0.0013
-
 7,8-diaminononanoate
-
 Escherichia coli
6.3.3.3 0.005
-
 ATP
-
 Escherichia coli
6.3.3.3 0.6
-
 Mg2+
-
 Escherichia coli
6.3.3.3 3.4
-
 CO2
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.3.3 42000
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.3.3.3 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.3.3
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.3.3 additional information
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.3.3 ATP + 7,8-diaminononanoate + CO2
-
 Escherichia coli ADP + phosphate + dethiobiotin
-
 ?
6.3.3.3 ATP + 7,8-diaminononanoate + CO2 CO2 in form of HCO3- Escherichia coli ADP + phosphate + dethiobiotin
-
 ?
6.3.3.3 ATP + diaminobiotin + CO2 37% of the activity relative to 7,8-diaminononanoate Escherichia coli ADP + phosphate + biotin
-
 ?
6.3.3.3 CTP + 7,8-diaminononanoate + CO2
-
 Escherichia coli CDP + phosphate + dethiobiotin
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.3.3 dimer
-
 Escherichia coli