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Literature summary extracted from

  • Izumi, Y.; Kano, Y.; Inagaki, K.; Kawase, N.; Tani, Y.; Yamada, H.
    Characterization of biotin biosynthetic enzymes of Bacillus sphaericus: a dethiobiotin producing bacterium (1981), Agric. Biol. Chem., 45, 1983-1989.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.1.6 Cd2+ 1 mM, almost complete inhibition Lysinibacillus sphaericus
2.8.1.6 Co2+ 1 mM, almost complete inhibition Lysinibacillus sphaericus
2.8.1.6 Cu2+ 1 mM, almost complete inhibition Lysinibacillus sphaericus
2.8.1.6 Hg2+ 1 mM, almost complete inhibition Lysinibacillus sphaericus
2.8.1.6 Zn2+ 1 mM, almost complete inhibition Lysinibacillus sphaericus
6.3.3.3 Ag+ 1 mM, 100% inhibition Lysinibacillus sphaericus
6.3.3.3 Cd2+ 1 mM, 70% inhibition Lysinibacillus sphaericus
6.3.3.3 Co2+ 1 mM, 35% inhibition Lysinibacillus sphaericus
6.3.3.3 Cu2+ 1 mM, 100% inhibition Lysinibacillus sphaericus
6.3.3.3 Hg2+ 1 mM, 100% inhibition Lysinibacillus sphaericus
6.3.3.3 Ni2+ 1 mM, 80% inhibition Lysinibacillus sphaericus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.3.3 0.045
-
 7,8-diaminononanoate
-
 Lysinibacillus sphaericus
6.3.3.3 0.42
-
 ATP
-
 Lysinibacillus sphaericus
6.3.3.3 10
-
 CO2
-
 Lysinibacillus sphaericus
6.3.3.3 40
-
 MgCl2
-
 Lysinibacillus sphaericus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.47 additional information effects of several metal ions on biotin biosynthesis Lysinibacillus sphaericus
6.3.3.3 Mg2+ required Escherichia coli
6.3.3.3 Mg2+ required Lysinibacillus sphaericus
6.3.3.3 Mg2+ Km: 40 mM MgCl2 Lysinibacillus sphaericus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.47 6-carboxyhexanoyl-CoA + L-alanine Lysinibacillus sphaericus i.e. pimeloyl-CoA 8-amino-7-oxononanoate + CoA + CO2 i.e. 7-oxo-8-amino-pelargonic acid ?
2.3.1.47 6-carboxyhexanoyl-CoA + L-alanine Lipomyces starkeyi i.e. pimeloyl-CoA 8-amino-7-oxononanoate + CoA + CO2 i.e. 7-oxo-8-amino-pelargonic acid ?
2.3.1.47 6-carboxyhexanoyl-CoA + L-alanine Sporidiobolus salmonicolor i.e. pimeloyl-CoA 8-amino-7-oxononanoate + CoA + CO2 i.e. 7-oxo-8-amino-pelargonic acid ?
2.3.1.47 6-carboxyhexanoyl-CoA + L-alanine Sporobolomyces coprophilus i.e. pimeloyl-CoA 8-amino-7-oxononanoate + CoA + CO2 i.e. 7-oxo-8-amino-pelargonic acid ?
2.3.1.47 6-carboxyhexanoyl-CoA + L-alanine Lysinibacillus sphaericus reaction in biotin biosynthesis 8-amino-7-oxononanoate + CoA + CO2 i.e. 7-oxo-8-amino-pelargonic acid ?
2.3.1.47 6-carboxyhexanoyl-CoA + L-alanine Lipomyces starkeyi reaction in biotin biosynthesis 8-amino-7-oxononanoate + CoA + CO2 i.e. 7-oxo-8-amino-pelargonic acid ?
2.3.1.47 6-carboxyhexanoyl-CoA + L-alanine Sporidiobolus salmonicolor reaction in biotin biosynthesis 8-amino-7-oxononanoate + CoA + CO2 i.e. 7-oxo-8-amino-pelargonic acid ?
2.3.1.47 6-carboxyhexanoyl-CoA + L-alanine Sporobolomyces coprophilus reaction in biotin biosynthesis 8-amino-7-oxononanoate + CoA + CO2 i.e. 7-oxo-8-amino-pelargonic acid ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.47 Lipomyces starkeyi
-
-
-
2.3.1.47 Lysinibacillus sphaericus
-
-
-
2.3.1.47 Lysinibacillus sphaericus IFO 3525
-
-
-
2.3.1.47 Sporidiobolus salmonicolor
-
-
-
2.3.1.47 Sporobolomyces coprophilus
-
-
-
2.8.1.6 Lysinibacillus sphaericus
-
-
-
6.3.3.3 Alcaligenes faecalis
-
-
-
6.3.3.3 Bacillus roseus
-
-
-
6.3.3.3 Corynebacterium glutamicum
-
-
-
6.3.3.3 Escherichia coli
-
-
-
6.3.3.3 Klebsiella aerogenes
-
-
-
6.3.3.3 Klebsiella pneumoniae
-
-
-
6.3.3.3 Lipomyces starkeyi
-
-
-
6.3.3.3 Lysinibacillus sphaericus
-
-
-
6.3.3.3 Priestia megaterium
-
-
-
6.3.3.3 Pseudomonas taetrolens
-
-
-
6.3.3.3 Rhodotorula glutinis
-
-
-
6.3.3.3 Saccharomyces kloeckerianus
-
-
-
6.3.3.3 Sporidiobolus salmonicolor
-
-
-
6.3.3.3 Sporobolomyces coprophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.3.3
-
 Lysinibacillus sphaericus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.47 additional information
-
 crude cell-extracts from various organism Lysinibacillus sphaericus
2.3.1.47 additional information
-
 crude cell-extracts from various organism Lipomyces starkeyi
2.3.1.47 additional information
-
 crude cell-extracts from various organism Sporidiobolus salmonicolor
2.3.1.47 additional information
-
 crude cell-extracts from various organism Sporobolomyces coprophilus
6.3.3.3 additional information
-
-
 Escherichia coli
6.3.3.3 additional information
-
-
 Lysinibacillus sphaericus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.47 6-carboxyhexanoyl-CoA + L-alanine i.e. pimeloyl-CoA Lysinibacillus sphaericus 8-amino-7-oxononanoate + CoA + CO2 i.e. 7-oxo-8-amino-pelargonic acid ?
2.3.1.47 6-carboxyhexanoyl-CoA + L-alanine i.e. pimeloyl-CoA Lipomyces starkeyi 8-amino-7-oxononanoate + CoA + CO2 i.e. 7-oxo-8-amino-pelargonic acid ?
2.3.1.47 6-carboxyhexanoyl-CoA + L-alanine i.e. pimeloyl-CoA Sporidiobolus salmonicolor 8-amino-7-oxononanoate + CoA + CO2 i.e. 7-oxo-8-amino-pelargonic acid ?
2.3.1.47 6-carboxyhexanoyl-CoA + L-alanine i.e. pimeloyl-CoA Sporobolomyces coprophilus 8-amino-7-oxononanoate + CoA + CO2 i.e. 7-oxo-8-amino-pelargonic acid ?
2.3.1.47 6-carboxyhexanoyl-CoA + L-alanine reaction in biotin biosynthesis Lysinibacillus sphaericus 8-amino-7-oxononanoate + CoA + CO2 i.e. 7-oxo-8-amino-pelargonic acid ?
2.3.1.47 6-carboxyhexanoyl-CoA + L-alanine reaction in biotin biosynthesis Lipomyces starkeyi 8-amino-7-oxononanoate + CoA + CO2 i.e. 7-oxo-8-amino-pelargonic acid ?
2.3.1.47 6-carboxyhexanoyl-CoA + L-alanine reaction in biotin biosynthesis Sporidiobolus salmonicolor 8-amino-7-oxononanoate + CoA + CO2 i.e. 7-oxo-8-amino-pelargonic acid ?
2.3.1.47 6-carboxyhexanoyl-CoA + L-alanine reaction in biotin biosynthesis Sporobolomyces coprophilus 8-amino-7-oxononanoate + CoA + CO2 i.e. 7-oxo-8-amino-pelargonic acid ?
2.8.1.6 dethiobiotin + sulfur
-
 Lysinibacillus sphaericus biotin
-
 ?
6.3.3.3 ADP + 7,8-diaminononanoate + CO2 5% of the activity relative to ATP Lysinibacillus sphaericus AMP + phosphate + dethiobiotin
-
 ?
6.3.3.3 ADP + 7,8-diaminononanoate + CO2 5% of the activity relative to ATP Lysinibacillus sphaericus IFO 3525 AMP + phosphate + dethiobiotin
-
 ?
6.3.3.3 ATP + 7,8-diaminononanoate + CO2
-
 Alcaligenes faecalis ADP + phosphate + dethiobiotin
-
 ?
6.3.3.3 ATP + 7,8-diaminononanoate + CO2
-
 Escherichia coli ADP + phosphate + dethiobiotin
-
 ?
6.3.3.3 ATP + 7,8-diaminononanoate + CO2
-
 Priestia megaterium ADP + phosphate + dethiobiotin
-
 ?
6.3.3.3 ATP + 7,8-diaminononanoate + CO2
-
 Lysinibacillus sphaericus ADP + phosphate + dethiobiotin
-
 ?
6.3.3.3 ATP + 7,8-diaminononanoate + CO2
-
 Klebsiella aerogenes ADP + phosphate + dethiobiotin
-
 ?
6.3.3.3 ATP + 7,8-diaminononanoate + CO2
-
 Saccharomyces kloeckerianus ADP + phosphate + dethiobiotin
-
 ?
6.3.3.3 ATP + 7,8-diaminononanoate + CO2
-
 Lipomyces starkeyi ADP + phosphate + dethiobiotin
-
 ?
6.3.3.3 ATP + 7,8-diaminononanoate + CO2
-
 Sporidiobolus salmonicolor ADP + phosphate + dethiobiotin
-
 ?
6.3.3.3 ATP + 7,8-diaminononanoate + CO2
-
 Sporobolomyces coprophilus ADP + phosphate + dethiobiotin
-
 ?
6.3.3.3 ATP + 7,8-diaminononanoate + CO2
-
 Rhodotorula glutinis ADP + phosphate + dethiobiotin
-
 ?
6.3.3.3 ATP + 7,8-diaminononanoate + CO2
-
 Klebsiella pneumoniae ADP + phosphate + dethiobiotin
-
 ?
6.3.3.3 ATP + 7,8-diaminononanoate + CO2
-
 Bacillus roseus ADP + phosphate + dethiobiotin
-
 ?
6.3.3.3 ATP + 7,8-diaminononanoate + CO2
-
 Corynebacterium glutamicum ADP + phosphate + dethiobiotin
-
 ?
6.3.3.3 ATP + 7,8-diaminononanoate + CO2
-
 Pseudomonas taetrolens ADP + phosphate + dethiobiotin
-
 ?
6.3.3.3 ATP + 7,8-diaminononanoate + CO2 CO2 in form of HCO3- Lysinibacillus sphaericus ADP + phosphate + dethiobiotin
-
 ?
6.3.3.3 ATP + 7,8-diaminononanoate + CO2
-
 Lysinibacillus sphaericus IFO 3525 ADP + phosphate + dethiobiotin
-
 ?
6.3.3.3 ATP + 7,8-diaminononanoate + CO2 CO2 in form of HCO3- Lysinibacillus sphaericus IFO 3525 ADP + phosphate + dethiobiotin
-
 ?
6.3.3.3 ITP + 7,8-diaminononanoate + CO2 8.6% of the activity relative to ATP Lysinibacillus sphaericus IDP + phosphate + dethiobiotin
-
 ?
6.3.3.3 ITP + 7,8-diaminononanoate + CO2 8.6% of the activity relative to ATP Lysinibacillus sphaericus IFO 3525 IDP + phosphate + dethiobiotin
-
 ?
6.3.3.3 UTP + 7,8-diaminononanoate + CO2 7% of the activity relative to ATP Lysinibacillus sphaericus UDP + phosphate + dethiobiotin
-
 ?
6.3.3.3 UTP + 7,8-diaminononanoate + CO2 7% of the activity relative to ATP Lysinibacillus sphaericus IFO 3525 UDP + phosphate + dethiobiotin
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.1.6 37
-
-
 Lysinibacillus sphaericus
6.3.3.3 40
-
-
 Lysinibacillus sphaericus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.8.1.6 35 40 35°C: about 70% of maximal activity, 40°C: about 60% of maximal activity Lysinibacillus sphaericus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.1.6 8
-
-
 Lysinibacillus sphaericus
6.3.3.3 7.5
-
-
 Lysinibacillus sphaericus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.8.1.6 7 9 pH 7: about 50% of maximal activity, pH 9: about 60% of maximal activity Lysinibacillus sphaericus