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Literature summary extracted from

  • Birnberg, P.R.; Rao, S.S.; Lippincott, J.A.
    Octopine dehydrogenase of a Vinca rosea crown gall-tumor (1983), Phytochemistry, 22, 1345-1355.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.5.1.11 the apoenzyme is protected against inactivation by NADP+ Catharanthus roseus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.11 Methyl octopine
-
 Catharanthus roseus
1.5.1.11 NADH substrate inhibition Catharanthus roseus
1.5.1.11 NADP+
-
 Catharanthus roseus
1.5.1.11 NADPH substrate inhibition Catharanthus roseus
1.5.1.11 pyruvate substrate inhibition Catharanthus roseus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.11 additional information
-
 additional information dependence of the Km-values for Arg and pyruvate on the concentration of the other substrates Catharanthus roseus
1.5.1.11 0.001 0.01 NADPH
-
 Catharanthus roseus
1.5.1.11 0.015
-
 NADP+
-
 Catharanthus roseus
1.5.1.11 0.7
-
 L-Orn with pyruvate and NADPH as cosubstrates Catharanthus roseus
1.5.1.11 1
-
 oxaloacetate L-Lys, with pyruvate and NADPH as cosubstrates Catharanthus roseus
1.5.1.11 2.7
-
 L-Gln with pyruvate and NADPH as cosubstrates Catharanthus roseus
1.5.1.11 3.9
-
 L-canavanine with pyruvate and NADPH as cosubstrates Catharanthus roseus
1.5.1.11 5.1
-
 L-Arg with pyruvate and NADPH as cosubstrate Catharanthus roseus
1.5.1.11 5.4
-
 L-Cys with pyruvate and NADPH as cosubstrate Catharanthus roseus
1.5.1.11 22
-
 L-citrulline with pyruvate and NADPH as cosubstrates Catharanthus roseus
1.5.1.11 22
-
 L-citrulline L-Met, with pyruvate and NADPH as cosubstrate Catharanthus roseus
1.5.1.11 23
-
 L-His with pyruvate and NADPH as cosubstrate Catharanthus roseus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.11 additional information Catharanthus roseus the enzyme may synthesize lysopine, octopinic acid, and the corresponding Nalpha-derivatives of glutamine ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.11 Catharanthus roseus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.11 crown-gall tumor-tissue induced by Agrobacterium tumefaciens Catharanthus roseus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.11 crown gall cell lines of Catharanthus roseus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.11 Canavanine + pyruvate + NADPH at 56% of the activity with L-Arg Catharanthus roseus N2-(D-1-Carboxyethyl)-canavanine + NADP+ + H2O
-
 ?
1.5.1.11 Homoarginine + pyruvate + NADPH at 62% of the activity with L-Arg Catharanthus roseus N2-(D-1-Carboxyethyl)-homoarginine + NADP+ + H2O
-
 ?
1.5.1.11 L-Arg + 2-oxobutanoate + NADPH at 21% of the activity with pyruvate Catharanthus roseus N2-(D-2-Carboxypropyl)-L-Arg + NADP+ + H2O
-
 ?
1.5.1.11 L-Arg + glyoxylate + NADPH at 5% of the activity with pyruvate Catharanthus roseus ?
-
 ?
1.5.1.11 L-Arg + oxaloacetate + NADH
-
 Catharanthus roseus ?
-
 ?
1.5.1.11 L-Arg + pyruvate + NADH
-
 Catharanthus roseus N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
 ?
1.5.1.11 L-Arg + pyruvate + NADPH
-
 Catharanthus roseus N2-(D-1-Carboxyethyl)-L-Arg + NADP+ + H2O
-
 ?
1.5.1.11 L-canavanine + pyruvate + NADPH + H+
-
 Catharanthus roseus N2-(D-1-carboxyethyl)-canavanine + NADP+ + H2O
-
 r
1.5.1.11 L-Citrulline + pyruvate + NADPH
-
 Catharanthus roseus N2-(D-1-Carboxyethyl)-L-citrulline + NADP+ + H2O
-
 ?
1.5.1.11 L-Cys + pyruvate + NADPH at 27% of the activity with L-Arg Catharanthus roseus N2-(D-1-Carboxyethyl)-L-Cys + NADP+ + H2O
-
 ?
1.5.1.11 L-Gln + pyruvate + NADPH at 18% of the activity with L-Arg Catharanthus roseus N2-(D-1-Carboxyethyl)-L-Gln + NADP+ + H2O
-
 ?
1.5.1.11 L-His + pyruvate + NADPH at 78% of the activity with L-Arg Catharanthus roseus N2-(D-1-Carboxyethyl)-L-His + NADP+ + H2O
-
 ?
1.5.1.11 L-Lys + pyruvate + NADPH at 31% of the activity with L-Arg Catharanthus roseus Lysopine + NADP+ + H2O
-
 ?
1.5.1.11 L-Met + pyruvate + NADPH at 67% of the activity with L-Arg Catharanthus roseus N2-(D-1-Carboxyethyl)-L-Met + NADP+ + H2O
-
 ?
1.5.1.11 L-serine + pyruvate + NADPH at 25% of the activity with L-Arg Catharanthus roseus N2-(D-1-carboxyethyl)-L-Ser + NADP+ + H2O
-
 ?
1.5.1.11 additional information the enzyme may synthesize lysopine, octopinic acid, and the corresponding Nalpha-derivatives of glutamine Catharanthus roseus ?
-
 ?
1.5.1.11 Orn + pyruvate + NADPH at 30% of the activity with L-Arg Catharanthus roseus N2-(D-1-Carboxyethyl)-L-Orn + NADP+ + H2O
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.11 5.2
-
 with Lys or Orn as substrate Catharanthus roseus
1.5.1.11 6 6.6 with Arg or His as substrate Catharanthus roseus
1.5.1.11 9.2
-
 octopine oxidation Catharanthus roseus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.5.1.11 6.5 8.5 4°C, stable. Loses most of its activity after 24 h at lower or higher pH-values Catharanthus roseus

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.11 NADH
-
 Catharanthus roseus
1.5.1.11 NADPH
-
 Catharanthus roseus