EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.11 | epsilon-aminohexanoate | - |
Helianthus annuus | |
1.5.1.11 | NADP+ | uncompetitive with respect to pyruvate | Helianthus annuus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.11 | 1.85 | - |
L-Orn | - |
Helianthus annuus | |
1.5.1.11 | 8.8 | - |
L-Arg | - |
Helianthus annuus | |
1.5.1.11 | 11.2 | - |
L-Met | - |
Helianthus annuus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.5.1.11 | 38000 | - |
1 * 38000, SDS-PAGE | Helianthus annuus |
1.5.1.11 | 39000 | - |
gel filtration | Helianthus annuus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.1.11 | Helianthus annuus | - |
crown-gall tumor-tissue induced by Agrobacterium tumefaciens | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.5.1.11 | crown-gall tumor-tissue induced by Agrobacterium tumefaciens | Helianthus annuus |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.5.1.11 | N2-(D-1-carboxyethyl)-L-arginine + NAD+ + H2O = L-arginine + pyruvate + NADH + H+ | partially ordered mechanism. NADPH binds first followed by the random order, rapid-equilibrium-binding of Arg and pyruvate. Orn probably binds before pyruvate | Helianthus annuus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.5.1.11 | crown gall | induced by Agrobacterium tumefaciens | Helianthus annuus | - |
1.5.1.11 | crown gall | the structural gene for octopine synthase is located in a central portion of the T-DNA of Agrobacterium tumefaciens Ti plasmid | Helianthus annuus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.5.1.11 | additional information | - |
- |
Helianthus annuus |
EC Number | Storage Stability | Organism |
---|---|---|
1.5.1.11 | -30°C, buffer containing glycerol, NADPH and 2-mercaptoethanol, stable for many weeks | Helianthus annuus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.11 | L-Arg + pyruvate + NADH | - |
Helianthus annuus | N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O | - |
? | |
1.5.1.11 | L-Arg + pyruvate + NADPH | - |
Helianthus annuus | N2-(D-1-Carboxyethyl)-L-Arg + NADP+ + H2O | - |
? | |
1.5.1.11 | L-Gln + pyruvate + NADPH | - |
Helianthus annuus | N2-(D-1-Carboxyethyl)-L-Gln + NADP+ + H2O | - |
? | |
1.5.1.11 | L-Lys + pyruvate + NADPH | - |
Helianthus annuus | Lysopine + NADP+ + H2O | - |
? | |
1.5.1.11 | L-Met + pyruvate + NADPH | - |
Helianthus annuus | N2-(D-1-Carboxyethyl)-L-Met + NADP+ + H2O | - |
? | |
1.5.1.11 | Orn + pyruvate + NADH | - |
Helianthus annuus | N2-(D-1-Carboxyethyl)-L-Orn + NAD+ + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.5.1.11 | monomer | 1 * 38000, SDS-PAGE | Helianthus annuus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.11 | NADH | - |
Helianthus annuus | |
1.5.1.11 | NADPH | - |
Helianthus annuus |