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Literature summary extracted from

  • Bertrand, R.; MacKenzie, R.E.; Jolivot, J.
    Human liver methenyltetrahydrofolate synthetase: improved purification and increased affinity for folate polyglutamate substrates (1987), Biochim. Biophys. Acta, 911, 154-161.
    View publication on PubMed

General Stability

EC Number General Stability Organism
6.3.3.2 non-ionic detergent, 0.1% v/v Tween 20, and 10% v/v glycerol stabilize the enzyme which is extremely labile at 4 C Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.3.2 4-amino-N10-methylpteroyl pentaglutamate i.e. methotrexate pentaglutamate Homo sapiens
6.3.3.2 dihydropteroyl pentaglutamate potent Homo sapiens
6.3.3.2 dihydropteroyl-L-Glu weak Homo sapiens
6.3.3.2 additional information no inhibition by methotrexate Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.3.2 0.0006
-
 (6S)-5-formyltetrahydropteroyl-pentaglutamate
-
 Homo sapiens
6.3.3.2 0.002
-
 (6S)-5-formyltetrahydrofolate
-
 Homo sapiens
6.3.3.2 0.004
-
 (6R,S)-5-formyltetrahydrofolate
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.3.2 25000
-
 gel filtration Homo sapiens
6.3.3.2 27000
-
 gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
6.3.3.2 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.3.2
-
 Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
6.3.3.2 ATP + 5-formyltetrahydrofolate = ADP + phosphate + 5,10-methenyltetrahydrofolate sequential mechanism Homo sapiens
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.3.2 liver
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.3.2 37
-
-
 Homo sapiens

Storage Stability

EC Number Storage Stability Organism
6.3.3.2 -80°C, pH 7.0, 0.1% v/v Tween 20, 10% v/v glycerol, stable for several months Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.3.2 ATP + (6R,S)-5-formyltetrahydrofolate
-
 Homo sapiens ADP + phosphate + 5,10-methenyltetrahydrofolate
-
 ?
6.3.3.2 ATP + (6S)-5-formyltetrahydropteroyl-pentaglutamate
-
 Homo sapiens ?
-
 ?
6.3.3.2 ATP + 5-formyltetrahydrofolate no reverse reaction detected Homo sapiens ADP + phosphate + 5,10-methylenetetrahydrofolate
-
 ?
6.3.3.2 ATP + 5-formyltetrahydrofolate (6R,S)-5-formyltetrahydrofolate, (6S)-5-formyltetrahydrofolate Homo sapiens ADP + phosphate + 5,10-methylenetetrahydrofolate
-
 ?
6.3.3.2 ATP + 5-formyltetrahydropteroyl-pentaglutamate
-
 Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.3.2 monomer 1 * 27000, SDS-PAGE Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.3.3.2 4
-
 extremely labile at Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.3.2 additional information
-
 additional information
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.3.2 6.5
-
 cytosolic enzyme Homo sapiens