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Literature summary extracted from

  • Bouhss, A.; Mengin-Lecreulx, D.; Blanot, D.; van Heijenoort, J.; Parquet, C.
    Invariant amino acids in the Mur peptide synthetases of bacterial peptidoglycan synthesis and their modification by site-directed mutagenesis in the UDP-MurNAc;L-alanine ligase from Escherichia coli (1997), Biochemistry, 36, 11556-11563.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
6.3.2.8 H199A mutant enzymes K130A, H199A, N293A, N296A, and R327A lead to important variations of the Km value for one or more substrates Escherichia coli
6.3.2.8 K130A mutant enzymes K130A, H199A, N293A, N296A, and R327A lead to important variations of the Km value for one or more substrates Escherichia coli
6.3.2.8 N293A mutant enzymes K130A, H199A, N293A, N296A, and R327A lead to important variations of the Km value for one or more substrates Escherichia coli
6.3.2.8 N296A mutant enzymes K130A, H199A, N293A, N296A, and R327A lead to important variations of the Km value for one or more substrates Escherichia coli
6.3.2.8 R327A mutant enzymes K130A, H199A, N293A, N296A, and R327A lead to important variations of the Km value for one or more substrates Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.8 additional information
-
 additional information Km-value of wild-type and mutant enzymes Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.8 Escherichia coli
-
 wild-type and mutant enzymes
-

Reaction

EC Number Reaction Comment Organism Reaction ID
6.3.2.8 ATP + UDP-N-acetyl-alpha-D-muramate + L-alanine = ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine the binding order of the substrates can be ATP, UDP-N-acetylmuramate, and Ala Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.8 ATP + UDP-N-acetylmuramate + L-Ala
-
 Escherichia coli ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala
-
 ?