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Literature summary extracted from

  • Liger, S.; Masson, A.; Blanot, D.; van Heijenoort, J.; Parquet, C.
    Over-production, purification and properties of the uridine-diphosphate-N-acetylmuramate:L-alanine ligase from Escherichia coli (1995), Eur. J. Biochem., 230, 80-87.
    View publication on PubMed

General Stability

EC Number General Stability Organism
6.3.2.8 presence of 2-mercaptoethanol and glycerol is essential for stability Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.2.8 3,3,3-Trifluoro-DL-Ala
-
 Escherichia coli
6.3.2.8 3,3-Difluoro-L-Ala
-
 Escherichia coli
6.3.2.8 3-chloro-L-Ala
-
 Escherichia coli
6.3.2.8 3-Cyano-L-Ala
-
 Escherichia coli
6.3.2.8 3-Fluoro-L-Ala
-
 Escherichia coli
6.3.2.8 L-2-aminobutyrate
-
 Escherichia coli
6.3.2.8 L-Allylglycine weak Escherichia coli
6.3.2.8 L-Cys weak Escherichia coli
6.3.2.8 L-propargylglycine
-
 Escherichia coli
6.3.2.8 L-Vinylglycine
-
 Escherichia coli
6.3.2.8 potassium phosphate no effect up to 20 mM, significant decrease of activity at higher concentrations Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.8 0.02
-
 L-Ala
-
 Escherichia coli
6.3.2.8 0.1
-
 UDP-N-acetylmuramate alpha anomer of UDP-N-acetylmuramate Escherichia coli
6.3.2.8 0.43
-
 UDP-N-acetylglucosyl-enolpyruvate
-
 Escherichia coli
6.3.2.8 0.45
-
 ATP
-
 Escherichia coli
6.3.2.8 1
-
 dihydro-UDP-N-acetylmuramate
-
 Escherichia coli
6.3.2.8 2
-
 UDP-N-acetyl-beta-D-muramate
-
 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.2.8 Mg2+ optimal concentration: 10-20 mM Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.2.8 50000
-
 x * 50000, SDS-PAGE Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.8 Escherichia coli
-
 overproduced in strains harbouring the recombinant plasmids bearing the mur gene under the control of the lac promoter
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.8
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.2.8 2.94
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
6.3.2.8 -20°C, 20 mM potassium phosphate, pH 7.2, 1 mM EDTA, 2.5 mM 2-mercaptoethanol, 15% glycerol Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.8 ATP + dihydro-UDP-N-acetylmuramate + L-Ala
-
 Escherichia coli ADP + phosphate + dihydro-UDP-N-acetylmuramoyl-L-Ala
-
 ?
6.3.2.8 ATP + UDP-N-acetyl-beta-D-muramate + L-alanine
-
 Escherichia coli ADP + phosphate + UDP-N-acetyl-beta-D-muramoyl-L-alanine
-
 ?
6.3.2.8 ATP + UDP-N-acetylglucosaminyl-enoylpyruvate + L-Ala
-
 Escherichia coli ADP + phosphate + UDP-N-acetylglucosaminyl-enoylpyruvoyl-L-Ala
-
 ?
6.3.2.8 ATP + UDP-N-acetylglucosyl-enolpyruvate + L-alanine
-
 Escherichia coli ADP + phosphate + UDP-N-acetylglucosyl-enolpyruvoyl-L-alanine
-
 ?
6.3.2.8 ATP + UDP-N-acetylmuramate + DL-Ser
-
 Escherichia coli ADP + phosphate + UDP-N-acetylmuramoyl-DL-Ser
-
 ?
6.3.2.8 ATP + UDP-N-acetylmuramate + Gly
-
 Escherichia coli ADP + phosphate + UDP-N-acetylmuramoyl-Gly
-
 ?
6.3.2.8 ATP + UDP-N-acetylmuramate + L-Ala r Escherichia coli ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala
-
 ?
6.3.2.8 ATP + UDP-N-acetylmuramate + L-Ala alpha- and beta-anomer of UDP-N-acetylmuramate Escherichia coli ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala
-
 ?
6.3.2.8 additional information enzyme catalyzes an exchange reaction leading to the formation of 4% of the UDP-N-acetylmuramoyl-L-[14C]Ala with UDP-N-acetylmuramoyl-L-Ala, L-[14C]Ala, ADP and phosphate Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.2.8 ? x * 50000, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.2.8 45
-
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.2.8 additional information
-
 additional information
-
 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.2.8 8.6
-
-
 Escherichia coli