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Literature summary extracted from

  • Mizuno, Y.; Yaegashi, M.; Ito, E.
    Purification and properties of uridine diphosphate N-acetylmuramate:L-alanine ligase (1973), J. Biochem., 74, 525-538.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.3.2.8 2-mercaptoethanol stimulates Staphylococcus aureus
6.3.2.8 2-mercaptoethanol maximal concentration 2.5 mM Staphylococcus aureus

General Stability

EC Number General Stability Organism
6.3.2.8 decrease in stability at high salt concentrations is prevented by addition of 10% glycerol and 2.5 mM 2-mercaptoethanol Staphylococcus aureus
6.3.2.8 relatively stable at low ionic strength, unstable at high concentrations of salt Staphylococcus aureus

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.2.8 Ba2+ inhibits activation by Mn2+ Staphylococcus aureus
6.3.2.8 Ca2+ inhibits activation by Mn2+ Staphylococcus aureus
6.3.2.8 Cd2+ inhibits activation by Mn2+ Staphylococcus aureus
6.3.2.8 Co2+ inhibits activation by Mn2+ Staphylococcus aureus
6.3.2.8 Fe2+ inhibits activation by Mn2+ Staphylococcus aureus
6.3.2.8 Hg2+ inhibits activation by Mn2+ Staphylococcus aureus
6.3.2.8 Mg2+ inhibits activation by Mn2+ Staphylococcus aureus
6.3.2.8 Ni2+ inhibits activation by Mn2+ Staphylococcus aureus
6.3.2.8 Zn2+ inhibits activation by Mn2+ Staphylococcus aureus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.8 0.051
-
 UDP-N-acetylmuramate
-
 Staphylococcus aureus
6.3.2.8 0.15
-
 L-Ala
-
 Staphylococcus aureus
6.3.2.8 29
-
 Gly
-
 Staphylococcus aureus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.2.8 K+ monovalent cation required, NH4+ or K+ Staphylococcus aureus
6.3.2.8 Mg2+ divalent cation required, Mg2+ or Mn2+ Staphylococcus aureus
6.3.2.8 Mn2+ divalent cation required Staphylococcus aureus
6.3.2.8 Mn2+ Mg2+ or Mn2+, maximal activity at concentrations of Mn2+ equimolar to those of ATP Staphylococcus aureus
6.3.2.8 NH4+ monovalent cation required, NH4+ or K+ Staphylococcus aureus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.2.8 40000
-
 gel filtration in absence and in presence of 2.5 mM 2-mercaptoethanol Staphylococcus aureus

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.8 Staphylococcus aureus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.8
-
 Staphylococcus aureus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.2.8 0.14
-
-
 Staphylococcus aureus

Storage Stability

EC Number Storage Stability Organism
6.3.2.8 -18°C, 5 mM potassium phosphate, 10-20% loss of activity after 3 months Staphylococcus aureus
6.3.2.8 4°C, 5 mM potassium phosphate, overnight, 10-20% loss of activity. At 4°C, 250 mM potassium phosphate, 12 h, 30% loss of activity Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.8 ATP + UDP-N-acetylmuramate + DL-Ser
-
 Staphylococcus aureus ADP + phosphate + UDP-N-acetylmuramoyl-DL-Ser
-
 ?
6.3.2.8 ATP + UDP-N-acetylmuramate + Gly
-
 Staphylococcus aureus ADP + phosphate + UDP-N-acetylmuramoyl-Gly
-
 ?
6.3.2.8 ATP + UDP-N-acetylmuramate + L-Ala not active with D-Ala Staphylococcus aureus ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala
-
 ?
6.3.2.8 UTP + UDP-N-acetylmuramate + L-Ala about 11% of the activity relative to ATP Staphylococcus aureus UDP + phosphate + UDP-N-acetylmuramoyl-L-Ala
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.3.2.8 50
-
 5 mM potassium phosphate, pH 7.2, 20 min, 10-20% loss of activity. 60-90% loss of activity in 250 mM potassium phosphate buffer Staphylococcus aureus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.2.8 7.8 8.5
-
 Staphylococcus aureus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.3.2.8 7.5 9 pH 7.5: about 60% of maximal activity, pH 9.0: about 70% of maximal activity Staphylococcus aureus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
6.3.2.8 7.2
-
 5 mM potassium phosphate, 50°C, 20 min, 10-20% loss of activity. 60-90% loss of activity in 250 mM potassium phosphate buffer Staphylococcus aureus