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Literature summary extracted from

  • Bauder, R.; Tshisuaka, B.; Lingens, F.
    VII. Quinoline oxidoreductase from Pseudomonas putida a molybdenum-containing enzyme (1990), Biol. Chem. Hoppe-Seyler, 371, 1137-1144.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.3.99.17 ethanol 1 M, increases activity by about 10% Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.99.17 CN-
-
 Pseudomonas putida
1.3.99.17 methanol 0.7 M, 50% inhibition Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.99.17 0.182
-
 Quinoline with 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride as cosubstrate Pseudomonas putida
1.3.99.17 1.08
-
 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride with quinoline as cosubstrate Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.3.99.17 Fe contains 8 atoms of iron and 8 atoms of acid-labile sulfide Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.99.17 20000
-
 x * 20000 + x * 30000 + x * 85000, SDS-PAGE Pseudomonas putida
1.3.99.17 30000
-
 x * 20000 + x * 30000 + x * 85000, SDS-PAGE Pseudomonas putida
1.3.99.17 85000
-
 x * 20000 + x * 30000 + x * 85000, SDS-PAGE Pseudomonas putida
1.3.99.17 300000
-
 gel filtration Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.3.99.17 Pseudomonas putida
-
-
-
1.3.99.17 Pseudomonas putida 86
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.99.17
-
 Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.99.17 13.2
-
-
 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.99.17 4-chloroquinoline + p-iodonitrotetrazolium violet
-
 Pseudomonas putida ?
-
 ?
1.3.99.17 5-hydroxyquinoline + acceptor acceptor: 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride Pseudomonas putida ?
-
 ?
1.3.99.17 5-hydroxyquinoline + acceptor acceptors: 3,3'-(3,3'-dimethoxy-4,4'-bi-phenylene)-bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride], 2,6-dichlorophenolindophenol, phenazine-methosulfate, methylene blue Pseudomonas putida ?
-
 ?
1.3.99.17 5-hydroxyquinoline + acceptor 15% of the activity with quinoline Pseudomonas putida ?
-
 ?
1.3.99.17 5-hydroxyquinoline + acceptor acceptor: 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride Pseudomonas putida 86 ?
-
 ?
1.3.99.17 5-hydroxyquinoline + acceptor acceptors: 3,3'-(3,3'-dimethoxy-4,4'-bi-phenylene)-bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride], 2,6-dichlorophenolindophenol, phenazine-methosulfate, methylene blue Pseudomonas putida 86 ?
-
 ?
1.3.99.17 6-hydroxyquinoline + acceptor acceptor: 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride Pseudomonas putida ?
-
 ?
1.3.99.17 6-hydroxyquinoline + acceptor acceptors: 3,3'-(3,3'-dimethoxy-4,4'-bi-phenylene)-bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride], 2,6-dichlorophenolindophenol, phenazine-methosulfate, methylene blue Pseudomonas putida ?
-
 ?
1.3.99.17 6-hydroxyquinoline + acceptor 19% of the activity with quinoline Pseudomonas putida ?
-
 ?
1.3.99.17 7-hydroxyquinoline + acceptor acceptor: 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride Pseudomonas putida ?
-
 ?
1.3.99.17 7-hydroxyquinoline + acceptor acceptor: 3,3'-(3,3'-dimethoxy-4,4'-bi-phenylene)-bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride], 2,6-dichlorophenolindophenol, phenazine-methosulfate, methylene blue Pseudomonas putida ?
-
 ?
1.3.99.17 8-chloroquinoline + acceptor acceptor: 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride Pseudomonas putida ?
-
 ?
1.3.99.17 8-chloroquinoline + acceptor acceptors: 3,3'-(3,3'-dimethoxy-4,4'-bi-phenylene)-bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride], 2,6-dichlorophenolindophenol, phenazine-methosulfate, methylene blue Pseudomonas putida ?
-
 ?
1.3.99.17 8-hydroxyquinoline + acceptor acceptor: 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride Pseudomonas putida ?
-
 ?
1.3.99.17 8-hydroxyquinoline + acceptor acceptors: 3,3'-(3,3'-dimethoxy-4,4'-bi-phenylene)-bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride], 2,6-dichlorophenolindophenol, phenazine-methosulfate, methylene blue Pseudomonas putida ?
-
 ?
1.3.99.17 quinoline + acceptor acceptor: 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride Pseudomonas putida 2-oxo-1,2-dihydroquinoline + reduced acceptor
-
 ?
1.3.99.17 quinoline + acceptor acceptors: 3,3'-(3,3'-dimethoxy-4,4'-bi-phenylene)-bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride] Pseudomonas putida 2-oxo-1,2-dihydroquinoline + reduced acceptor
-
 ?
1.3.99.17 quinoline + acceptor 2,6-dichlorophenolindophenol, phenazine methosulfate, methylene blue Pseudomonas putida 2-oxo-1,2-dihydroquinoline + reduced acceptor
-
 ?
1.3.99.17 quinoline + acceptor acceptor: 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride Pseudomonas putida 86 2-oxo-1,2-dihydroquinoline + reduced acceptor
-
 ?
1.3.99.17 quinoline + acceptor acceptors: 3,3'-(3,3'-dimethoxy-4,4'-bi-phenylene)-bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride] Pseudomonas putida 86 2-oxo-1,2-dihydroquinoline + reduced acceptor
-
 ?
1.3.99.17 quinoline + acceptor 2,6-dichlorophenolindophenol, phenazine methosulfate, methylene blue Pseudomonas putida 86 2-oxo-1,2-dihydroquinoline + reduced acceptor
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.99.17 ? x * 20000 + x * 30000 + x * 85000, SDS-PAGE Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.99.17 FAD enzyme contains 2 moleculs of FAD Pseudomonas putida
1.3.99.17 molybdopterin
-
 Pseudomonas putida
1.3.99.17 additional information NAD+ and NADP+ are ineffective as cofactors Pseudomonas putida