EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.1.53 | 0.043 | - |
NAD+ | - |
Comamonas testosteroni | |
1.3.1.53 | 0.09 | - |
(1R,2S)-1,2-Dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate | - |
Comamonas testosteroni |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.3.1.53 | Fe2+ | activates | Comamonas testosteroni |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.3.1.53 | 39000 | - |
2 * 39000, SDS-PAGE | Comamonas testosteroni |
1.3.1.53 | 60000 | - |
- |
Comamonas testosteroni |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.53 | (1R,2S)-1,2-Dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate + NAD+ | Comamonas testosteroni | the enzyme catalyzes the last step specific to the degradation of p-toluate to protocatechuate | Protocatechuic acid + NADH + CO2 | - |
? | |
1.3.1.53 | (1R,2S)-1,2-Dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate + NAD+ | Comamonas testosteroni T-2 | the enzyme catalyzes the last step specific to the degradation of p-toluate to protocatechuate | Protocatechuic acid + NADH + CO2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.1.53 | Comamonas testosteroni | - |
T-2 | - |
1.3.1.53 | Comamonas testosteroni T-2 | - |
T-2 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.3.1.53 | - |
Comamonas testosteroni |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.3.1.53 | culture condition:p-toluate-grown cell | - |
Comamonas testosteroni | - |
1.3.1.53 | culture condition:terephthalate-grown cell | - |
Comamonas testosteroni | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.3.1.53 | additional information | - |
- |
Comamonas testosteroni |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.53 | (1R,2S)-1,2-Dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate + NAD+ | the hydride ion is transferred to the si face of NAD+, i.e. B-specific | Comamonas testosteroni | Protocatechuic acid + NADH + CO2 | - |
? | |
1.3.1.53 | (1R,2S)-1,2-Dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate + NAD+ | the enzyme catalyzes the last step specific to the degradation of p-toluate to protocatechuate | Comamonas testosteroni | Protocatechuic acid + NADH + CO2 | - |
? | |
1.3.1.53 | (1R,2S)-1,2-Dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate + NAD+ | the hydride ion is transferred to the si face of NAD+, i.e. B-specific | Comamonas testosteroni T-2 | Protocatechuic acid + NADH + CO2 | - |
? | |
1.3.1.53 | (1R,2S)-1,2-Dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate + NAD+ | the enzyme catalyzes the last step specific to the degradation of p-toluate to protocatechuate | Comamonas testosteroni T-2 | Protocatechuic acid + NADH + CO2 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.3.1.53 | dimer | 2 * 39000, SDS-PAGE | Comamonas testosteroni |