EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.3.8.5 | (E)-2-methyl-2-butenoyl-CoA | - |
Ascaris suum | |
1.3.8.5 | acetoacetyl-CoA | - |
Ascaris suum | |
1.3.8.5 | Ag2+ | - |
Ascaris suum | |
1.3.8.5 | crotonyl-CoA | - |
Ascaris suum | |
1.3.8.5 | decanoyl-CoA | - |
Ascaris suum | |
1.3.8.5 | hexanoyl-CoA | - |
Ascaris suum | |
1.3.8.5 | Hg2+ | - |
Ascaris suum | |
1.3.8.5 | NEM | little effect | Ascaris suum | |
1.3.8.5 | p-hydroxymercuribenzoate | little effect | Ascaris suum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.8.5 | 0.018 | - |
2-methylbutanoyl-CoA | - |
Ascaris suum | |
1.3.8.5 | 0.021 | - |
2-methylpentanoyl-CoA | - |
Ascaris suum |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.3.8.5 | mitochondrion | - |
Ascaris suum | 5739 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.3.8.5 | 42500 | - |
4 * 42500, SDS-PAGE | Ascaris suum |
1.3.8.5 | 170000 | - |
gel filtration | Ascaris suum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.8.5 | additional information | Ascaris suum | the electron-transport chain of the organism and the enzyme 2-methyl-branched-chain-enoyl-CoA reductase are responsible for the NADH-dependent reduction of the branched-chain enoyl-CoAs observed in mitochondria | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.8.5 | Ascaris suum | O16843 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.3.8.5 | - |
Ascaris suum |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.3.8.5 | 1.58 | - |
2-methylpentanoyl-CoA | Ascaris suum |
1.3.8.5 | 1.62 | - |
2-methylbutanoyl-CoA | Ascaris suum |
EC Number | Storage Stability | Organism |
---|---|---|
1.3.8.5 | -20°C, 50 mM potassium phosphate buffer, pH 7.4, 10% glycerol, stable for at least 30 days | Ascaris suum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.8.5 | 2-methylbutanoyl-CoA + electron-transfer flavoprotein | - |
Ascaris suum | (E)-2-methylbut-2-enoyl-CoA + reduced electron-transfer flavoprotein + H+ | - |
? | |
1.3.8.5 | 2-methylpentanoyl-CoA + electron-transfer flavoprotein | 98% of the activity with 2-methylbutanoyl-CoA | Ascaris suum | 2-methyl-2-pentenoyl-CoA + reduced electron-transfer flavoprotein + H+ | - |
? | |
1.3.8.5 | butyryl-CoA + electron-transfer flavoprotein | 13.6% of the activity with 2-methylbutanoyl-CoA | Ascaris suum | ? + reduced electron-transfer flavoprotein + H+ | - |
? | |
1.3.8.5 | additional information | no reaction with: propionyl-CoA, isobutanoyl-CoA, isopentanoyl-CoA, palmitoyl-CoA | Ascaris suum | ? | - |
? | |
1.3.8.5 | additional information | the electron-transport chain of the organism and the enzyme 2-methyl-branched-chain-enoyl-CoA reductase are responsible for the NADH-dependent reduction of the branched-chain enoyl-CoAs observed in mitochondria | Ascaris suum | ? | - |
? | |
1.3.8.5 | octanoyl-CoA + electron-transfer flavoprotein | 12.8% of the activity with 2-methylbutanoyl-CoA | Ascaris suum | ? + reduced electron-transfer flavoprotein + H+ | - |
? | |
1.3.8.5 | pentanoyl-CoA + electron-transfer flavoprotein | 14.4% of the activity with 2-methylbutanoyl-CoA | Ascaris suum | ? + reduced electron-transfer flavoprotein + H+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.3.8.5 | tetramer | 4 * 42500, SDS-PAGE | Ascaris suum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.3.8.5 | 7.5 | - |
- |
Ascaris suum |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.3.8.5 | FAD | contains 0.9 mol of FAD per mol of subunit | Ascaris suum |