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Literature summary extracted from

  • Nagao, K.; Takizawa, N.; Kiyohara, H.
    Purification and properties of cis-phenanthrene dihydrodiol dehydrogenase in Alcaligenes faecalis AFK2 (1988), Agric. Biol. Chem., 52, 2621-2623.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.49 Hg2+
-
 Alcaligenes faecalis
1.3.1.49 PCMB
-
 Alcaligenes faecalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.49 0.021
-
 (+)-cis-3,4-dihydrophenanthrene-3,4-diol
-
 Alcaligenes faecalis
1.3.1.49 0.058
-
 NAD+
-
 Alcaligenes faecalis
1.3.1.49 0.63
-
 NADP+
-
 Alcaligenes faecalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.49 27500
-
 4 * 27500, SDS-PAGE Alcaligenes faecalis
1.3.1.49 100000
-
 gel filtration Alcaligenes faecalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.49 (+)-cis-3,4-dihydrophenanthrene-3,4-diol + NAD+ Alcaligenes faecalis enzyme catalyzes an early step in the degradation of phenanthrene phenanthrene-3,4-diol + NADH
-
 ?
1.3.1.49 (+)-cis-3,4-dihydrophenanthrene-3,4-diol + NAD+ Alcaligenes faecalis AFK2 enzyme catalyzes an early step in the degradation of phenanthrene phenanthrene-3,4-diol + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.49 Alcaligenes faecalis
-
-
-
1.3.1.49 Alcaligenes faecalis AFK2
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.49
-
 Alcaligenes faecalis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.49 70.7
-
-
 Alcaligenes faecalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.49 (+)-cis-3,4-dihydrophenanthrene-3,4-diol + NAD+
-
 Alcaligenes faecalis phenanthrene-3,4-diol + NADH
-
 ?
1.3.1.49 (+)-cis-3,4-dihydrophenanthrene-3,4-diol + NAD+ enzyme catalyzes an early step in the degradation of phenanthrene Alcaligenes faecalis phenanthrene-3,4-diol + NADH
-
 ?
1.3.1.49 (+)-cis-3,4-dihydrophenanthrene-3,4-diol + NAD+
-
 Alcaligenes faecalis AFK2 phenanthrene-3,4-diol + NADH
-
 ?
1.3.1.49 (+)-cis-3,4-dihydrophenanthrene-3,4-diol + NAD+ enzyme catalyzes an early step in the degradation of phenanthrene Alcaligenes faecalis AFK2 phenanthrene-3,4-diol + NADH
-
 ?
1.3.1.49 (+)-cis-3,4-dihydrophenanthrene-3,4-diol + NADP+
-
 Alcaligenes faecalis phenanthrene-3,4-diol + NADPH + H+
-
 r
1.3.1.49 (+)-cis-3,4-dihydrophenanthrene-3,4-diol + NADP+
-
 Alcaligenes faecalis AFK2 phenanthrene-3,4-diol + NADPH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.3.1.49 tetramer 4 * 27500, SDS-PAGE Alcaligenes faecalis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.49 40
-
-
 Alcaligenes faecalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.49 9
-
-
 Alcaligenes faecalis

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.49 NAD+ electron acceptor Alcaligenes faecalis
1.3.1.49 NADP+ electron acceptor Alcaligenes faecalis