Literature summary extracted from

Gali, R.R.; Board, P.G.
Identification of an essential cysteine residue in human glutathione synthase (1997), Biochem. J., 321, 207-210.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
6.3.2.3	-	Homo sapiens

Protein Variants

EC Number	Protein Variants	Comment	Organism
6.3.2.3	C294A	mutant enzymes Cys294Ala and Cys409Ala retain significant residual activity. Substantial decreases in activity are detected with mutant Cys522Ala and Cys-free mutant Cys294/Cys409/Cys422 to Ala294/Ala409/Ala422	Homo sapiens
6.3.2.3	C409A	mutant enzymes Cys294Ala and Cys409Ala retain significant residual activity. Substantial decreases in activity are detected with mutant Cys522Ala and Cys-free mutant Cys294/Cys409/Cys422 to Ala294/Ala409/Ala422	Homo sapiens
6.3.2.3	C522A	mutant enzymes Cys294Ala and Cys409Ala retain significant residual activity. Substantial decreases in activity are detected with mutant Cys522Ala and Cys-free mutant Cys294/Cys409/Cys422 to Ala294/Ala409/Ala422	Homo sapiens

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
6.3.2.3	5,5'-dithiobis(2-nitrobenzoate)	-	Homo sapiens
6.3.2.3	iodoacetate	-	Homo sapiens
6.3.2.3	NEM	-	Homo sapiens
6.3.2.3	PCMB	-	Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
6.3.2.3	ATP + gamma-Glu-L-Cys + Gly	Homo sapiens	-	?	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
6.3.2.3	Homo sapiens	-	wild-type and mutant enzymes: Cys294Ala, Cys409Ala, Cys422Ala and Cys294/Cys409/Cys422 to Ala294/Ala409/Ala422	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
6.3.2.3	ATP + gamma-Glu-L-Cys + Gly	-	Homo sapiens	ADP + phosphate + glutathione	-	?
6.3.2.3	ATP + gamma-Glu-L-Cys + Gly	-	Homo sapiens	?	-	?

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Release 2023.1 (January 2023)