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Literature summary extracted from

  • Apontoweil, P.; Berends, W.
    Glutathione biosynthesis in Escherichia coli K12. Properties of the enzymes and regulation (1975), Biochim. Biophys. Acta, 399, 1-9.
    View publication on PubMed

General Stability

EC Number General Stability Organism
6.3.2.3 several successive freezings and thawings of the cell-free extract have no effect on enzyme activity Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.2.3 gamma-Glu-Cys above 2 mM Escherichia coli
6.3.2.3 GSSG no inhibition by reduced glutathione Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.3 0.4
-
 Gly
-
 Escherichia coli
6.3.2.3 0.63
-
 gamma-Glu-Cys
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.3 ATP + gamma-Glu-L-Cys + Gly Escherichia coli glutathione biosynthesis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.3 Escherichia coli
-
 K12, strain AB 1157
-

Storage Stability

EC Number Storage Stability Organism
6.3.2.3 -20°C, 20% loss of activity after 2 months Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.3 ATP + gamma-Glu-L-Cys + Gly
-
 Escherichia coli ADP + phosphate + glutathione i.e. gamma-Glu-Cys-Gly ?
6.3.2.3 ATP + gamma-Glu-L-Cys + Gly glutathione biosynthesis Escherichia coli ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.2.3 8.5
-
-
 Escherichia coli