BRENDA - Enzyme Database

Purification and some properties of the tungsten-containing carboxylic acid reductase from Clostridium formicoaceticum

White, H.; Feicht, R.; Huber, C.; Lottspeich, F.; Simon, H.; Biol. Chem. Hoppe-Seyler 372, 999-1005 (1991)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.99.6
0.8
-
Butanoate
-
Clostridium formicaceticum
1.2.99.6
1.2
-
4-Chlorobenzoate
-
Clostridium formicaceticum
1.2.99.6
1.4
-
4-Fluorobenzoate
-
Clostridium formicaceticum
1.2.99.6
2.1
-
4-Methoxybenzoate
-
Clostridium formicaceticum
1.2.99.6
3.8
-
3-chlorobenzoate
-
Clostridium formicaceticum
1.2.99.6
5
-
4-Methylbenzoate
-
Clostridium formicaceticum
1.2.99.6
6.3
-
propanoate
3-fluorobenzoate
Clostridium formicaceticum
1.2.99.6
6.4
-
propanoate
-
Clostridium formicaceticum
1.2.99.6
12.8
-
3-methylbenzoate
-
Clostridium formicaceticum
1.2.99.6
16.7
-
benzoate
-
Clostridium formicaceticum
1.2.99.6
44.9
-
3-Methoxybenzoate
-
Clostridium formicaceticum
1.2.99.6
50.4
-
3-hydroxybenzoate
-
Clostridium formicaceticum
1.2.99.6
382
-
4-hydroxybenzoate
-
Clostridium formicaceticum
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.2.99.6
Iron
contains 11 mol of iron per mol of enzyme
Clostridium formicaceticum
1.2.99.6
Tungsten
contains 1.4 mol of tungsten per mol of enzyme
Clostridium formicaceticum
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.99.6
67000
-
2 * 67000, SDS-PAGE
Clostridium formicaceticum
1.2.99.6
125000
-
gel filtration
Clostridium formicaceticum
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.2.99.6
Clostridium formicaceticum
-
-
-
Oxidation Stability
EC Number
Oxidation Stability
Organism
1.2.99.6
22% loss of activity after 5 min in air, at 4C
Clostridium formicaceticum
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
1.2.99.6
-
Clostridium formicaceticum
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1.2.99.6
additional information
-
-
Clostridium formicaceticum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.2.99.6
3-Chlorobenzoate + reduced carbamoyl methyl viologen
-
11943
Clostridium formicaceticum
3-Chlorobenzaldehyde + carbamoyl methyl viologen
-
-
-
?
1.2.99.6
3-Fluorobenzoate + reduced carbamoyl methyl viologen
-
11943
Clostridium formicaceticum
3-Fluorobenzaldehyde + carbamoyl methyl viologen
-
-
-
?
1.2.99.6
3-Hydroxybenzoate + reduced carbamoyl methyl viologen
-
11943
Clostridium formicaceticum
3-Hydroxybenzaldehyde + carbamoyl methyl viologen
-
-
-
?
1.2.99.6
3-Methoxybenzoate + reduced carbamoyl methyl viologen
-
11943
Clostridium formicaceticum
3-Methoxybenzaldhyde + carbamoyl methyl viologen
-
-
-
?
1.2.99.6
3-Methylbenzoate + reduced carbamoyl methyl viologen
-
11943
Clostridium formicaceticum
3-Methylbenzaldehyde + carbamoyl methyl viologen
-
-
-
?
1.2.99.6
4-Chlorobenzoate + reduced carbamoyl methyl viologen
-
11943
Clostridium formicaceticum
4-Chlorobenzaldehyde + carbamoyl methyl viologen
-
-
-
?
1.2.99.6
4-Fluorobenzoate + reduced carbamoyl methyl viologen
-
11943
Clostridium formicaceticum
4-Fluorobenzaldehyde + carbamoyl methyl viologen
-
-
-
?
1.2.99.6
4-Hydroxybenzoate + reduced carbamoyl methyl viologen
-
11943
Clostridium formicaceticum
4-Hydroxybenzaldehyde + carbamoyl methyl viologen
-
-
-
?
1.2.99.6
4-Methoxybenzoate + reduced carbamoyl methyl viologen
-
11943
Clostridium formicaceticum
4-Methoxybenzaldehyde + carbamoyl methyl viologen
-
-
-
?
1.2.99.6
4-Methylbenzoate + reduced carbamoyl methyl viologen
-
11943
Clostridium formicaceticum
4-Methylbenzaldehyde + carbamoyl methyl viologen
-
-
-
?
1.2.99.6
Benzaldehyde + carbamoyl methyl viologen
only reverse reaction described
11943
Clostridium formicaceticum
Benzoate + reduced carbamoyl methyl viologen
-
-
-
?
1.2.99.6
Butanal + NH2CO-methyl viologen
only reverse reaction described
11943
Clostridium formicaceticum
Butanoate + reduced NH2CO-methyl viologen
-
-
-
?
1.2.99.6
Propanal + benzyl viologen
only reverse reaction described
11943
Clostridium formicaceticum
Propanoate + reduced benzyl viologen
-
11943
Clostridium formicaceticum
?
1.2.99.6
Propanal + methyl viologen
only reverse reaction described
11943
Clostridium formicaceticum
Propanoate + reduced methyl viologen
-
11943
Clostridium formicaceticum
?
1.2.99.6
Propanal + NH2CO-methyl viologen
only reverse reaction described
11943
Clostridium formicaceticum
Propanoate + reduced carbamoyl methyl viologen
-
11943
Clostridium formicaceticum
?
1.2.99.6
Propanal + reduced 1,1',2,2'-tetramethylviologen
-
11943
Clostridium formicaceticum
Propanoate + 1,1',2,2'-tetramethylviologen
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.2.99.6
dimer
2 * 67000, SDS-PAGE
Clostridium formicaceticum
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.2.99.6
40
-
aldehyde dehydrogenation
Clostridium formicaceticum
Temperature Range [C]
EC Number
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
1.2.99.6
30
50
60% of maximal activity at 30C and 50C, inactive at 60C, aldehyde dehydrogenation
Clostridium formicaceticum
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.99.6
6
-
reduction of propanoate or benzoate
Clostridium formicaceticum
1.2.99.6
9
-
butanal and NH2-CO-methyl viologen as substrates
Clostridium formicaceticum
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.2.99.6
6
7
pH 6.0: optimum, pH 7.0: 25% of maximal activity, reduction of propanoate or benzoate
Clostridium formicaceticum
1.2.99.6
8
10
about 90% of maximal activity, butanal and NH2-CO-methyl viologen as substrates
Clostridium formicaceticum
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.2.99.6
pterin
contains 1.6 mol of pterin-6-carboxylic acid per mol of enzyme
Clostridium formicaceticum
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.2.99.6
pterin
contains 1.6 mol of pterin-6-carboxylic acid per mol of enzyme
Clostridium formicaceticum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.99.6
0.8
-
Butanoate
-
Clostridium formicaceticum
1.2.99.6
1.2
-
4-Chlorobenzoate
-
Clostridium formicaceticum
1.2.99.6
1.4
-
4-Fluorobenzoate
-
Clostridium formicaceticum
1.2.99.6
2.1
-
4-Methoxybenzoate
-
Clostridium formicaceticum
1.2.99.6
3.8
-
3-chlorobenzoate
-
Clostridium formicaceticum
1.2.99.6
5
-
4-Methylbenzoate
-
Clostridium formicaceticum
1.2.99.6
6.3
-
propanoate
3-fluorobenzoate
Clostridium formicaceticum
1.2.99.6
6.4
-
propanoate
-
Clostridium formicaceticum
1.2.99.6
12.8
-
3-methylbenzoate
-
Clostridium formicaceticum
1.2.99.6
16.7
-
benzoate
-
Clostridium formicaceticum
1.2.99.6
44.9
-
3-Methoxybenzoate
-
Clostridium formicaceticum
1.2.99.6
50.4
-
3-hydroxybenzoate
-
Clostridium formicaceticum
1.2.99.6
382
-
4-hydroxybenzoate
-
Clostridium formicaceticum
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.2.99.6
Iron
contains 11 mol of iron per mol of enzyme
Clostridium formicaceticum
1.2.99.6
Tungsten
contains 1.4 mol of tungsten per mol of enzyme
Clostridium formicaceticum
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.99.6
67000
-
2 * 67000, SDS-PAGE
Clostridium formicaceticum
1.2.99.6
125000
-
gel filtration
Clostridium formicaceticum
Oxidation Stability (protein specific)
EC Number
Oxidation Stability
Organism
1.2.99.6
22% loss of activity after 5 min in air, at 4C
Clostridium formicaceticum
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.99.6
-
Clostridium formicaceticum
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1.2.99.6
additional information
-
-
Clostridium formicaceticum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.2.99.6
3-Chlorobenzoate + reduced carbamoyl methyl viologen
-
11943
Clostridium formicaceticum
3-Chlorobenzaldehyde + carbamoyl methyl viologen
-
-
-
?
1.2.99.6
3-Fluorobenzoate + reduced carbamoyl methyl viologen
-
11943
Clostridium formicaceticum
3-Fluorobenzaldehyde + carbamoyl methyl viologen
-
-
-
?
1.2.99.6
3-Hydroxybenzoate + reduced carbamoyl methyl viologen
-
11943
Clostridium formicaceticum
3-Hydroxybenzaldehyde + carbamoyl methyl viologen
-
-
-
?
1.2.99.6
3-Methoxybenzoate + reduced carbamoyl methyl viologen
-
11943
Clostridium formicaceticum
3-Methoxybenzaldhyde + carbamoyl methyl viologen
-
-
-
?
1.2.99.6
3-Methylbenzoate + reduced carbamoyl methyl viologen
-
11943
Clostridium formicaceticum
3-Methylbenzaldehyde + carbamoyl methyl viologen
-
-
-
?
1.2.99.6
4-Chlorobenzoate + reduced carbamoyl methyl viologen
-
11943
Clostridium formicaceticum
4-Chlorobenzaldehyde + carbamoyl methyl viologen
-
-
-
?
1.2.99.6
4-Fluorobenzoate + reduced carbamoyl methyl viologen
-
11943
Clostridium formicaceticum
4-Fluorobenzaldehyde + carbamoyl methyl viologen
-
-
-
?
1.2.99.6
4-Hydroxybenzoate + reduced carbamoyl methyl viologen
-
11943
Clostridium formicaceticum
4-Hydroxybenzaldehyde + carbamoyl methyl viologen
-
-
-
?
1.2.99.6
4-Methoxybenzoate + reduced carbamoyl methyl viologen
-
11943
Clostridium formicaceticum
4-Methoxybenzaldehyde + carbamoyl methyl viologen
-
-
-
?
1.2.99.6
4-Methylbenzoate + reduced carbamoyl methyl viologen
-
11943
Clostridium formicaceticum
4-Methylbenzaldehyde + carbamoyl methyl viologen
-
-
-
?
1.2.99.6
Benzaldehyde + carbamoyl methyl viologen
only reverse reaction described
11943
Clostridium formicaceticum
Benzoate + reduced carbamoyl methyl viologen
-
-
-
?
1.2.99.6
Butanal + NH2CO-methyl viologen
only reverse reaction described
11943
Clostridium formicaceticum
Butanoate + reduced NH2CO-methyl viologen
-
-
-
?
1.2.99.6
Propanal + benzyl viologen
only reverse reaction described
11943
Clostridium formicaceticum
Propanoate + reduced benzyl viologen
-
11943
Clostridium formicaceticum
?
1.2.99.6
Propanal + methyl viologen
only reverse reaction described
11943
Clostridium formicaceticum
Propanoate + reduced methyl viologen
-
11943
Clostridium formicaceticum
?
1.2.99.6
Propanal + NH2CO-methyl viologen
only reverse reaction described
11943
Clostridium formicaceticum
Propanoate + reduced carbamoyl methyl viologen
-
11943
Clostridium formicaceticum
?
1.2.99.6
Propanal + reduced 1,1',2,2'-tetramethylviologen
-
11943
Clostridium formicaceticum
Propanoate + 1,1',2,2'-tetramethylviologen
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.2.99.6
dimer
2 * 67000, SDS-PAGE
Clostridium formicaceticum
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.2.99.6
40
-
aldehyde dehydrogenation
Clostridium formicaceticum
Temperature Range [C] (protein specific)
EC Number
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
1.2.99.6
30
50
60% of maximal activity at 30C and 50C, inactive at 60C, aldehyde dehydrogenation
Clostridium formicaceticum
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.99.6
6
-
reduction of propanoate or benzoate
Clostridium formicaceticum
1.2.99.6
9
-
butanal and NH2-CO-methyl viologen as substrates
Clostridium formicaceticum
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.2.99.6
6
7
pH 6.0: optimum, pH 7.0: 25% of maximal activity, reduction of propanoate or benzoate
Clostridium formicaceticum
1.2.99.6
8
10
about 90% of maximal activity, butanal and NH2-CO-methyl viologen as substrates
Clostridium formicaceticum