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Literature summary extracted from

  • White, H.; Feicht, R.; Huber, C.; Lottspeich, F.; Simon, H.
    Purification and some properties of the tungsten-containing carboxylic acid reductase from Clostridium formicoaceticum (1991), Biol. Chem. Hoppe-Seyler, 372, 999-1005.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.99.6 0.8
-
 Butanoate
-
 Clostridium formicaceticum
1.2.99.6 1.2
-
 4-Chlorobenzoate
-
 Clostridium formicaceticum
1.2.99.6 1.4
-
 4-Fluorobenzoate
-
 Clostridium formicaceticum
1.2.99.6 2.1
-
 4-Methoxybenzoate
-
 Clostridium formicaceticum
1.2.99.6 3.8
-
 3-chlorobenzoate
-
 Clostridium formicaceticum
1.2.99.6 5
-
 4-Methylbenzoate
-
 Clostridium formicaceticum
1.2.99.6 6.3
-
 propanoate 3-fluorobenzoate Clostridium formicaceticum
1.2.99.6 6.4
-
 propanoate
-
 Clostridium formicaceticum
1.2.99.6 12.8
-
 3-methylbenzoate
-
 Clostridium formicaceticum
1.2.99.6 16.7
-
 benzoate
-
 Clostridium formicaceticum
1.2.99.6 44.9
-
 3-Methoxybenzoate
-
 Clostridium formicaceticum
1.2.99.6 50.4
-
 3-hydroxybenzoate
-
 Clostridium formicaceticum
1.2.99.6 382
-
 4-hydroxybenzoate
-
 Clostridium formicaceticum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.99.6 Iron contains 11 mol of iron per mol of enzyme Clostridium formicaceticum
1.2.99.6 Tungsten contains 1.4 mol of tungsten per mol of enzyme Clostridium formicaceticum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.99.6 67000
-
 2 * 67000, SDS-PAGE Clostridium formicaceticum
1.2.99.6 125000
-
 gel filtration Clostridium formicaceticum

Organism

EC Number Organism UniProt Comment Textmining
1.2.99.6 Clostridium formicaceticum
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.2.99.6 22% loss of activity after 5 min in air, at 4°C Clostridium formicaceticum

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.99.6
-
 Clostridium formicaceticum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.99.6 additional information
-
-
 Clostridium formicaceticum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.99.6 3-Chlorobenzoate + reduced carbamoyl methyl viologen
-
 Clostridium formicaceticum 3-Chlorobenzaldehyde + carbamoyl methyl viologen
-
 ?
1.2.99.6 3-Fluorobenzoate + reduced carbamoyl methyl viologen
-
 Clostridium formicaceticum 3-Fluorobenzaldehyde + carbamoyl methyl viologen
-
 ?
1.2.99.6 3-Hydroxybenzoate + reduced carbamoyl methyl viologen
-
 Clostridium formicaceticum 3-Hydroxybenzaldehyde + carbamoyl methyl viologen
-
 ?
1.2.99.6 3-Methoxybenzoate + reduced carbamoyl methyl viologen
-
 Clostridium formicaceticum 3-Methoxybenzaldhyde + carbamoyl methyl viologen
-
 ?
1.2.99.6 3-Methylbenzoate + reduced carbamoyl methyl viologen
-
 Clostridium formicaceticum 3-Methylbenzaldehyde + carbamoyl methyl viologen
-
 ?
1.2.99.6 4-Chlorobenzoate + reduced carbamoyl methyl viologen
-
 Clostridium formicaceticum 4-Chlorobenzaldehyde + carbamoyl methyl viologen
-
 ?
1.2.99.6 4-Fluorobenzoate + reduced carbamoyl methyl viologen
-
 Clostridium formicaceticum 4-Fluorobenzaldehyde + carbamoyl methyl viologen
-
 ?
1.2.99.6 4-Hydroxybenzoate + reduced carbamoyl methyl viologen
-
 Clostridium formicaceticum 4-Hydroxybenzaldehyde + carbamoyl methyl viologen
-
 ?
1.2.99.6 4-Methoxybenzoate + reduced carbamoyl methyl viologen
-
 Clostridium formicaceticum 4-Methoxybenzaldehyde + carbamoyl methyl viologen
-
 ?
1.2.99.6 4-Methylbenzoate + reduced carbamoyl methyl viologen
-
 Clostridium formicaceticum 4-Methylbenzaldehyde + carbamoyl methyl viologen
-
 ?
1.2.99.6 Benzaldehyde + carbamoyl methyl viologen only reverse reaction described Clostridium formicaceticum Benzoate + reduced carbamoyl methyl viologen
-
 ?
1.2.99.6 Butanal + NH2CO-methyl viologen only reverse reaction described Clostridium formicaceticum Butanoate + reduced NH2CO-methyl viologen
-
 ?
1.2.99.6 Propanal + benzyl viologen only reverse reaction described Clostridium formicaceticum Propanoate + reduced benzyl viologen
-
 ?
1.2.99.6 Propanal + methyl viologen only reverse reaction described Clostridium formicaceticum Propanoate + reduced methyl viologen
-
 ?
1.2.99.6 Propanal + NH2CO-methyl viologen only reverse reaction described Clostridium formicaceticum Propanoate + reduced carbamoyl methyl viologen
-
 ?
1.2.99.6 Propanal + reduced 1,1',2,2'-tetramethylviologen
-
 Clostridium formicaceticum Propanoate + 1,1',2,2'-tetramethylviologen
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.99.6 dimer 2 * 67000, SDS-PAGE Clostridium formicaceticum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.99.6 40
-
 aldehyde dehydrogenation Clostridium formicaceticum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.2.99.6 30 50 60% of maximal activity at 30°C and 50°C, inactive at 60°C, aldehyde dehydrogenation Clostridium formicaceticum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.99.6 6
-
 reduction of propanoate or benzoate Clostridium formicaceticum
1.2.99.6 9
-
 butanal and NH2-CO-methyl viologen as substrates Clostridium formicaceticum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.99.6 6 7 pH 6.0: optimum, pH 7.0: 25% of maximal activity, reduction of propanoate or benzoate Clostridium formicaceticum
1.2.99.6 8 10 about 90% of maximal activity, butanal and NH2-CO-methyl viologen as substrates Clostridium formicaceticum

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.99.6 pterin contains 1.6 mol of pterin-6-carboxylic acid per mol of enzyme Clostridium formicaceticum