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Literature summary extracted from

  • Karrasch, M.; Börner, G.; Enssle, M.; Thauer, R.K.
    The molybdoenzyme formylmethanofuran dehydrogenase from Methanosarcina barkeri contains a pterin cofactor (1990), Eur. J. Biochem., 194, 367-372.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.2.7.12 enzyme in crude enzyme extract is unstable to freezing Methanosarcina barkeri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.7.12 0.02
-
 N-formylmethanofuran molybdenum enzyme Methanosarcina barkeri
1.2.7.12 0.02
-
 N-formylmethanofuran value below, methylviologen, molybdenum enzyme Methanosarcina barkeri

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.7.12 Iron contains 28 mol of non-heme iron and acid labile sulfur per mol of 220000 MW enzyme Methanosarcina barkeri
1.2.7.12 Molybdenum enzyme exists as molybdenum iron-sulfur protein or as tungsten iron-sulfur protein Methanosarcina barkeri
1.2.7.12 Molybdenum contains 0.5-0.8 mol of molybdenum per mol of 220000 MW enzyme Methanosarcina barkeri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.7.12 17000
-
 x * 65000 + x * 50000 + x * 37000 + x * 34000 + x * 29000 + x * 17000, SDS-PAGE Methanosarcina barkeri
1.2.7.12 29000
-
 x * 65000 + x * 50000 + x * 37000 + x * 34000 + x * 29000 + x * 17000, SDS-PAGE Methanosarcina barkeri
1.2.7.12 34000
-
 x * 65000 + x * 50000 + x * 37000 + x * 34000 + x * 29000 + x * 17000, SDS-PAGE Methanosarcina barkeri
1.2.7.12 37000
-
 x * 65000 + x * 50000 + x * 37000 + x * 34000 + x * 29000 + x * 17000, SDS-PAGE Methanosarcina barkeri
1.2.7.12 50000
-
 x * 65000 + x * 50000 + x * 37000 + x * 34000 + x * 29000 + x * 17000, SDS-PAGE Methanosarcina barkeri
1.2.7.12 65000
-
 x * 65000 + x * 50000 + x * 37000 + x * 34000 + x * 29000 + x * 17000, SDS-PAGE Methanosarcina barkeri
1.2.7.12 200000
-
 and MW 400000, native PAGE Methanosarcina barkeri
1.2.7.12 400000
-
 and MW 200000, native PAGE Methanosarcina barkeri
1.2.7.12 440000
-
 gel filtration Methanosarcina barkeri

Organism

EC Number Organism UniProt Comment Textmining
1.2.7.12 Methanosarcina barkeri
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.7.12
-
 Methanosarcina barkeri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.7.12 formylmethanofuran + oxidized methylviologen + H2O
-
 Methanosarcina barkeri methanofuran + reduced methylviologen + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.7.12 ? x * 65000 + x * 50000 + x * 37000 + x * 34000 + x * 29000 + x * 17000, SDS-PAGE Methanosarcina barkeri

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.7.12 70
-
-
 Methanosarcina barkeri

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.7.12 640
-
 formylmethanofuran
-
 Methanosarcina barkeri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.7.12 7.5
-
-
 Methanosarcina barkeri

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.7.12 pterin contains 0.6-0.9 mol of pterin per mol of 22000 MW enzyme Methanosarcina barkeri