Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Bertram, P.A.; Karrasch, M.; Schmitz, R.A.; B๖cher, R.; Albracht, S.P.J.; Thauer, R.K.
    Formylmethanofuran dehydrogenase from methanogenic archaea. Substrate specificity, EPR properties and reversible inactivation by cyanide of the molybdenum or tungsten iron-sulfur proteins (1994), Eur. J. Biochem., 220, 477-484.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.7.12 CN- molybdenum-containing enzymes are inactivated, tungsten-containing isoenzymes are not inactivated Methanosarcina barkeri
1.2.7.12 CN- molybdenum-containing enzymes are inactivated, tungsten-containing isoenzymes are not inactivated Methanothermobacter thermautotrophicus
1.2.7.12 CN- molybdenum-containing enzymes are inactivated, tungsten-containing isoenzymes are not inactivated Methanothermobacter wolfeii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.7.12 0.013
-
 N-formylmethanofuran molybdenum enzyme Methanothermobacter wolfeii
1.2.7.12 0.02
-
 N-formylmethanofuran
-
 Methanothermobacter thermautotrophicus
1.2.7.12 0.02
-
 N-formylmethanofuran molybdenum enzyme Methanosarcina barkeri
1.2.7.12 0.03
-
 N-formylmethanofuran
-
 Methanothermobacter thermautotrophicus
1.2.7.12 0.03
-
 N-formylmethanofuran
-
 Methanothermobacter wolfeii
1.2.7.12 0.035
-
 formate molybdenum enzyme Methanothermobacter wolfeii
1.2.7.12 0.053
-
 N-Furfurylformamide molybdenum enzyme Methanothermobacter wolfeii
1.2.7.12 1.7
-
 formate molybdenum enzyme Methanosarcina barkeri
1.2.7.12 3
-
 Formamide molybdenum enzyme Methanosarcina barkeri
1.2.7.12 5
-
 methylformamide molybdenum enzyme Methanosarcina barkeri

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.7.12 Molybdenum enzyme exists as molybdenum iron-sulfur protein or as tungsten iron-sulfur protein Methanosarcina barkeri
1.2.7.12 Molybdenum enzyme exists as molybdenum iron-sulfur protein or as tungsten iron-sulfur protein Methanothermobacter thermautotrophicus
1.2.7.12 Molybdenum enzyme exists as molybdenum iron-sulfur protein or as tungsten iron-sulfur protein Methanothermobacter wolfeii
1.2.7.12 Tungsten enzyme exists as molybdenum iron-sulfur protein or as tungsten iron-sulfur protein Methanothermobacter thermautotrophicus
1.2.7.12 Tungsten enzyme exists as molybdenum iron-sulfur protein or as tungsten iron-sulfur protein Methanothermobacter wolfeii

Organism

EC Number Organism UniProt Comment Textmining
1.2.7.12 Methanosarcina barkeri
-
-
-
1.2.7.12 Methanothermobacter thermautotrophicus
-
-
-
1.2.7.12 Methanothermobacter wolfeii
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.7.12 Formamide + oxidized acceptor molybdenum enzyme, 0.1% of the activity relative to formylmethanofuran Methanosarcina barkeri ?
-
 ?
1.2.7.12 Formate + oxidized acceptor molybdenum enzyme, 1% of the activity relative to formylmethanofuran Methanosarcina barkeri ?
-
 ?
1.2.7.12 Formate + oxidized acceptor molybdenum enzyme, 3% of the activity relative to formylmethanofuran Methanothermobacter wolfeii ?
-
 ?
1.2.7.12 formylmethanofuran + oxidized 1,1',2,2'-tetramethylviologen
-
 Methanosarcina barkeri CO2 + methanofuran + reduced 1,1',2,2'-tetramethylviologen
-
 ?
1.2.7.12 formylmethanofuran + oxidized 1,1',2,2'-tetramethylviologen tungsten enzymes are specific for N-formylmethanofuran Methanothermobacter thermautotrophicus CO2 + methanofuran + reduced 1,1',2,2'-tetramethylviologen
-
 ?
1.2.7.12 formylmethanofuran + oxidized 1,1',2,2'-tetramethylviologen tungsten enzymes are specific for N-formylmethanofuran Methanothermobacter wolfeii CO2 + methanofuran + reduced 1,1',2,2'-tetramethylviologen
-
 ?
1.2.7.12 formylmethanofuran + oxidized 1,1',2,2'-tetramethylviologen molybdenum enzyme is specific for N-formylmethanofuran Methanothermobacter thermautotrophicus CO2 + methanofuran + reduced 1,1',2,2'-tetramethylviologen
-
 ?
1.2.7.12 N-Furfurylformamide + oxidized acceptor
-
 Methanothermobacter thermautotrophicus ?
-
 ?
1.2.7.12 N-Furfurylformamide + oxidized acceptor molybdenum enzyme, 1% of the activity relative to formylmethanofuran Methanothermobacter wolfeii ?
-
 ?
1.2.7.12 N-Furfurylformamide + oxidized acceptor molybdenum enzyme, 11% of the activity relative to formylmethanofuran Methanosarcina barkeri ?
-
 ?
1.2.7.12 N-Methylformamide + oxidized acceptor molybdenum enzyme, 0.2% of the activity relative to formylmethanofuran Methanosarcina barkeri ?
-
 ?