BRENDA - Enzyme Database

Thermodynamics of the formylmethanofuran dehydrogenase reaction in Methanobacterium thermoautotrophicum

Bertram, P.A.; Thauer, R.K.; Eur. J. Biochem. 226, 811-818 (1994)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.7.12
0.008
-
oxidized carbamoylviologen
-
Methanothermobacter thermautotrophicus
1.2.7.12
0.015
-
oxidized benzylviologen
-
Methanothermobacter thermautotrophicus
1.2.7.12
0.03
-
N-formylmethanofuran
-
Methanothermobacter thermautotrophicus
1.2.7.12
0.04
-
oxidized methylviologen
-
Methanothermobacter thermautotrophicus
1.2.7.12
0.1
-
methanofuran
-
Methanothermobacter thermautotrophicus
1.2.7.12
0.125
-
oxidized 1,1',2,2'-tetramethylviologen
-
Methanothermobacter thermautotrophicus
1.2.7.12
0.4
-
reduced 1,1',2,2'-tetramethylviologen
-
Methanothermobacter thermautotrophicus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.2.7.12
additional information
Methanothermobacter thermautotrophicus
initial step of methanogenesis
?
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.2.7.12
Methanothermobacter thermautotrophicus
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.2.7.12
formylmethanofuran + H2O + oxidized carbamoylviologen
-
11920
Methanothermobacter thermautotrophicus
CO2 + methanofuran + reduced carbamoylviologen
-
-
-
r
1.2.7.12
formylmethanofuran + oxidized 1,1',2,2'-tetramethylviologen
r
11920
Methanothermobacter thermautotrophicus
CO2 + methanofuran + reduced 1,1',2,2'-tetramethylviologen
-
11920
Methanothermobacter thermautotrophicus
?
1.2.7.12
formylmethanofuran + oxidized benzylviologen + H2O
-
11920
Methanothermobacter thermautotrophicus
methanofuran + reduced benzylviologen + CO2
-
11920
Methanothermobacter thermautotrophicus
?
1.2.7.12
formylmethanofuran + oxidized carbamoylmethylviologen + H2O
-
11920
Methanothermobacter thermautotrophicus
methanofuran + reduced carbamoylmethylviologen + CO2
-
11920
Methanothermobacter thermautotrophicus
?
1.2.7.12
formylmethanofuran + oxidized methylviologen + H2O
-
11920
Methanothermobacter thermautotrophicus
methanofuran + reduced methylviologen + CO2
-
11920
Methanothermobacter thermautotrophicus
?
1.2.7.12
additional information
no reduction of metronidazole, methylene blue, FAD or coenzyme F420 with formylmethanofuran
11920
Methanothermobacter thermautotrophicus
?
-
-
-
?
1.2.7.12
additional information
initial step of methanogenesis
11920
Methanothermobacter thermautotrophicus
?
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.2.7.12
70
-
-
Methanothermobacter thermautotrophicus
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.2.7.12
25
70
25°C: less than 5% of maximal activity, 60°C: about 90% of maximal activity, 70°C: maximal activity
Methanothermobacter thermautotrophicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.7.12
0.008
-
oxidized carbamoylviologen
-
Methanothermobacter thermautotrophicus
1.2.7.12
0.015
-
oxidized benzylviologen
-
Methanothermobacter thermautotrophicus
1.2.7.12
0.03
-
N-formylmethanofuran
-
Methanothermobacter thermautotrophicus
1.2.7.12
0.04
-
oxidized methylviologen
-
Methanothermobacter thermautotrophicus
1.2.7.12
0.1
-
methanofuran
-
Methanothermobacter thermautotrophicus
1.2.7.12
0.125
-
oxidized 1,1',2,2'-tetramethylviologen
-
Methanothermobacter thermautotrophicus
1.2.7.12
0.4
-
reduced 1,1',2,2'-tetramethylviologen
-
Methanothermobacter thermautotrophicus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.2.7.12
additional information
Methanothermobacter thermautotrophicus
initial step of methanogenesis
?
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.2.7.12
formylmethanofuran + H2O + oxidized carbamoylviologen
-
11920
Methanothermobacter thermautotrophicus
CO2 + methanofuran + reduced carbamoylviologen
-
-
-
r
1.2.7.12
formylmethanofuran + oxidized 1,1',2,2'-tetramethylviologen
r
11920
Methanothermobacter thermautotrophicus
CO2 + methanofuran + reduced 1,1',2,2'-tetramethylviologen
-
11920
Methanothermobacter thermautotrophicus
?
1.2.7.12
formylmethanofuran + oxidized benzylviologen + H2O
-
11920
Methanothermobacter thermautotrophicus
methanofuran + reduced benzylviologen + CO2
-
11920
Methanothermobacter thermautotrophicus
?
1.2.7.12
formylmethanofuran + oxidized carbamoylmethylviologen + H2O
-
11920
Methanothermobacter thermautotrophicus
methanofuran + reduced carbamoylmethylviologen + CO2
-
11920
Methanothermobacter thermautotrophicus
?
1.2.7.12
formylmethanofuran + oxidized methylviologen + H2O
-
11920
Methanothermobacter thermautotrophicus
methanofuran + reduced methylviologen + CO2
-
11920
Methanothermobacter thermautotrophicus
?
1.2.7.12
additional information
no reduction of metronidazole, methylene blue, FAD or coenzyme F420 with formylmethanofuran
11920
Methanothermobacter thermautotrophicus
?
-
-
-
?
1.2.7.12
additional information
initial step of methanogenesis
11920
Methanothermobacter thermautotrophicus
?
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.2.7.12
70
-
-
Methanothermobacter thermautotrophicus
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.2.7.12
25
70
25°C: less than 5% of maximal activity, 60°C: about 90% of maximal activity, 70°C: maximal activity
Methanothermobacter thermautotrophicus