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Literature summary extracted from

  • Schröder, H.C.; Wehland, J.; Weber, K.
    Purification of brain tubulin-tyrosine ligase by biochemical and immunological methods (1985), J. Cell Biol., 100, 276-281.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.25 0.0254
-
L-Tyr
-
Sus scrofa
6.3.2.25 0.186
-
L-Dopa
-
Sus scrofa
6.3.2.25 0.432
-
L-Phe
-
Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.2.25 40000
-
glycerol density gradient centrifugation. The MW of the ligase preincubated with excess phosphocellulose-tubulin is 150000 Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.25 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.25
-
Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.2.25 brain
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.2.25 0.324
-
-
Sus scrofa

Storage Stability

EC Number Storage Stability Organism
6.3.2.25 -70°C, in presence of 40% or 20% glycerol the enzyme can be stored for at least 6 months at a protein concentration of 0.02 mg/ml Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.25 ATP + detyrosinated alpha-tubulin + L-DOPA
-
Sus scrofa ?
-
?
6.3.2.25 ATP + detyrosinated alpha-tubulin + L-Phe
-
Sus scrofa ?
-
?
6.3.2.25 ATP + detyrosinated alpha-tubulin + tyramine very low activity Sus scrofa ?
-
?

Subunits

EC Number Subunits Comment Organism
6.3.2.25 monomer 1 * 40000, SDS-PAGE Sus scrofa