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Literature summary extracted from

  • Raybin, D.; Flavin, M.
    Enzyme which specifically adds tyrosine to the alpha chain of tubulin (1977), Biochemistry, 16, 2189-2194.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.2.25 alpha-L-Glu-L-Tyr
-
 Bos taurus
6.3.2.25 L-Ala-L-Tyr
-
 Bos taurus
6.3.2.25 L-hydroxyphenylalanine
-
 Bos taurus
6.3.2.25 L-Phe
-
 Bos taurus
6.3.2.25 L-Tyr-Gly
-
 Bos taurus
6.3.2.25 L-Tyr-L-Ala
-
 Bos taurus
6.3.2.25 L-Tyr-L-Glu
-
 Bos taurus
6.3.2.25 L-Tyr-L-Tyr
-
 Bos taurus
6.3.2.25 tyramine
-
 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.25 0.002
-
 alpha-tubulin below 0.002 mM Bos taurus
6.3.2.25 0.045
-
 L-Tyr
-
 Bos taurus
6.3.2.25 0.16
-
 L-hydroxyphenylalanine
-
 Bos taurus
6.3.2.25 2.9
-
 L-Phe
-
 Bos taurus
6.3.2.25 8
-
 tyramine
-
 Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.2.25 35000
-
 gel filtration, MW of enzyme in crude extract, the MW of the purified enzyme is 150000. Addition of pure dimeric tubulin to the 35000 MW enzyme converts it back to the larger form which is apparently a stoichiometric 1:1 complex of tubulin and the 35000 MW enzyme Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.25 ATP + detyrosinated alpha-tubulin + L-Tyr Bos taurus posttranslational modification of tubulin with potential significance for the regulation of microtubule assembly or function ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.25 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.25
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.2.25 brain
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.2.25 0.039
-
-
 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.25 alpha-tubulin + ADP + phosphate
-
 Bos taurus ATP + detyrosinated alpha-tubulin + L-tyrosine
-
 r
6.3.2.25 ATP + detyrosinated alpha-tubulin + L-hydroxyphenylalanine
-
 Bos taurus ?
-
 ?
6.3.2.25 ATP + detyrosinated alpha-tubulin + L-Phe
-
 Bos taurus ?
-
 ?
6.3.2.25 ATP + detyrosinated alpha-tubulin + L-Tyr posttranslational modification of tubulin with potential significance for the regulation of microtubule assembly or function Bos taurus ?
-
 ?
6.3.2.25 ATP + detyrosinated alpha-tubulin + tyramine
-
 Bos taurus ?
-
 ?