BRENDA - Enzyme Database

In vitro biosynthesis of diphthamide, studied with mutant chinese hamster ovary cells resistant to diphtheria toxin

Moehring, T.J.; Danley, D.E.; Moehring, J.M.; Mol. Cell. Biol. 4, 642-650 (1984) View publication on PubMedView publication on EuropePMC

Data extracted from this reference:

Organism
EC Number
Organism
UniProt
Commentary
Textmining
6.3.1.14
Cricetulus griseus
-
CHO-K1
-
6.3.1.14
Cricetulus griseus CHO-K1
-
CHO-K1
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
6.3.1.14
partial
Cricetulus griseus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
6.3.1.14
ovary
-
Cricetulus griseus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
6.3.1.14
ATP + diphthine + ammonia
-
1162
Cricetulus griseus
ADP + phosphate + diphthamide
-
1162
Cricetulus griseus
?
6.3.1.14
ATP + diphthine + ammonia
-
1162
Cricetulus griseus CHO-K1
ADP + phosphate + diphthamide
-
1162
Cricetulus griseus CHO-K1
?
6.3.1.14
CTP + diphthine + ammonia
11% of activity compared to ATP
1162
Cricetulus griseus
CDP + phosphate + diphthamide
-
-
-
?
6.3.1.14
CTP + diphthine + ammonia
11% of activity compared to ATP
1162
Cricetulus griseus CHO-K1
CDP + phosphate + diphthamide
-
-
-
?
6.3.1.14
GTP + diphthine + ammonia
14% of activity compared to ATP
1162
Cricetulus griseus
GDP + phosphate + diphthamide
-
-
-
?
6.3.1.14
GTP + diphthine + ammonia
14% of activity compared to ATP
1162
Cricetulus griseus CHO-K1
GDP + phosphate + diphthamide
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
6.3.1.14
30
-
assay at
Cricetulus griseus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
6.3.1.14
partial
Cricetulus griseus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
6.3.1.14
ovary
-
Cricetulus griseus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
6.3.1.14
ATP + diphthine + ammonia
-
1162
Cricetulus griseus
ADP + phosphate + diphthamide
-
1162
Cricetulus griseus
?
6.3.1.14
ATP + diphthine + ammonia
-
1162
Cricetulus griseus CHO-K1
ADP + phosphate + diphthamide
-
1162
Cricetulus griseus CHO-K1
?
6.3.1.14
CTP + diphthine + ammonia
11% of activity compared to ATP
1162
Cricetulus griseus
CDP + phosphate + diphthamide
-
-
-
?
6.3.1.14
CTP + diphthine + ammonia
11% of activity compared to ATP
1162
Cricetulus griseus CHO-K1
CDP + phosphate + diphthamide
-
-
-
?
6.3.1.14
GTP + diphthine + ammonia
14% of activity compared to ATP
1162
Cricetulus griseus
GDP + phosphate + diphthamide
-
-
-
?
6.3.1.14
GTP + diphthine + ammonia
14% of activity compared to ATP
1162
Cricetulus griseus CHO-K1
GDP + phosphate + diphthamide
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
6.3.1.14
30
-
assay at
Cricetulus griseus