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Literature summary extracted from

  • Fabry, S.; Hensel, R.
    Purification and characterization of D-glyceraldehyde-3-phosphate dehydrogenase from the thermophilic archaebacterium Methanothermus fervidus (1987), Eur. J. Biochem., 165, 147-155.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.2.1.13
-
 Methanothermus fervidus
1.2.1.59
-
 Methanothermus fervidus

General Stability

EC Number General Stability Organism
1.2.1.59 NADP+ stabilizes against thermal inactivation, NAD+ has no effect Methanothermus fervidus
1.2.1.59 salts stabilize against thermal inactivation in the order: potassium phosphate > sodium phosphate > K2SO4 > sodium citrate > KCl > NaCl Methanothermus fervidus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.59 additional information insensitive to pentalenolactone Methanothermus fervidus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.13 0.002
-
 NADPH
-
 Methanothermus fervidus
1.2.1.13 0.003
-
 NADP+
-
 Methanothermus fervidus
1.2.1.13 0.066
-
 NADH
-
 Methanothermus fervidus
1.2.1.13 0.65
-
 NAD+
-
 Methanothermus fervidus
1.2.1.13 43
-
 arsenate
-
 Methanothermus fervidus
1.2.1.13 53
-
 phosphate
-
 Methanothermus fervidus
1.2.1.59 0.002
-
 NADPH
-
 Methanothermus fervidus
1.2.1.59 0.003
-
 NADP+
-
 Methanothermus fervidus
1.2.1.59 0.066
-
 NADPH
-
 Methanothermus fervidus
1.2.1.59 0.65
-
 NAD+
-
 Methanothermus fervidus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.59 phosphate dependent on Methanothermus fervidus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.13 45000
-
 4 * 45000, SDS-PAGE Methanothermus fervidus
1.2.1.13 200000
-
 gel filtration Methanothermus fervidus
1.2.1.59 45000
-
 4 * 45000, SDS-PAGE Methanothermus fervidus
1.2.1.59 200000
-
 gel filtration Methanothermus fervidus

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.13 Methanothermus fervidus
-
-
-
1.2.1.59 Methanothermus fervidus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.13
-
 Methanothermus fervidus
1.2.1.59
-
 Methanothermus fervidus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.13 49.59
-
-
 Methanothermus fervidus
1.2.1.59 49.59
-
-
 Methanothermus fervidus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.13 D-glyceraldehyde 3-phosphate + arsenate + NADP+
-
 Methanothermus fervidus 3-phospho-D-glyceroyl arsenate + NADPH
-
 ?
1.2.1.13 D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Methanothermus fervidus 3-phospho-D-glyceroyl phosphate + NADH
-
 r
1.2.1.13 D-glyceraldehyde 3-phosphate + phosphate + NADP+
-
 Methanothermus fervidus 3-phospho-D-glyceroyl phosphate + NADPH
-
 r
1.2.1.59 D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Methanothermus fervidus 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 r
1.2.1.59 D-glyceraldehyde-3-phosphate + phosphate + NAD(P)+ r Methanothermus fervidus 1,3-diphosphoglycerate + NAD(P)H
-
 ?
1.2.1.59 D-glyceraldehyde-3-phosphate + phosphate + NADP+ r Methanothermus fervidus 1,3-diphosphoglycerate + NADPH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.13 tetramer 4 * 45000, SDS-PAGE Methanothermus fervidus
1.2.1.59 tetramer 4 * 45000, SDS-PAGE Methanothermus fervidus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.13 additional information
-
 the efficiency of salts stabilizing against thermal inactivation at a specific ionic strength decrease in the order: potassium phosphate, sodium phosphate, potassium sulfate, sodium sulfate, sodium citrate, potassium chloride, sodium chloride Methanothermus fervidus
1.2.1.13 75
-
 stable up to Methanothermus fervidus
1.2.1.13 90
-
 stabilized by NADP+ and at high ionic strength up to 90°C Methanothermus fervidus
1.2.1.59 75
-
 pH 7.0, 30 min, stable Methanothermus fervidus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.59 8.4
-
-
 Methanothermus fervidus

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.13 NAD+ cofactor Methanothermus fervidus
1.2.1.13 NADP+ cofactor Methanothermus fervidus