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Literature summary extracted from

  • Hirsch, W.; Schägger, H.; Fuchs, G.
    Phenylglyoxylate:NAD+ oxidoreductase (CoA benzoylating), a new enzyme of anaerobic phenylalanine metabolism in the denitrifying bacterium Azoarcus evansii (1998), Eur. J. Biochem., 251, 907-915.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.58 0.045
-
phenylglyoxylate
-
Aromatoleum evansii
1.2.1.58 0.055
-
CoA
-
Aromatoleum evansii
1.2.1.58 0.074
-
NAD+
-
Aromatoleum evansii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.58 Iron iron-sulfur protein, contains 35 mol Fe and 36 mol acid-labile sulfur per mol of enzyme Aromatoleum evansii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.58 11500
-
2 * 50000 + 2 * 48000 + 2 * 43000 + 2 * 24000 + 2 * 11500, SDS-PAGE. The enzyme complex consists of a core enzyme of 4 subunits with the composition alpha2beta2gamma2delta2, this complex is able to reduce viologen dyes. The holoenzyme contains in addition an epsilon2 unit that catalyzes the transfer of electrons from a small ferredoxin-like subunit of the core complex to NAD, this unit also catalyzes the transhydrogenase reaction, carries FAD and resembles ferredoxin:NAD(P)+-oxidoreductase Aromatoleum evansii
1.2.1.58 24000
-
2 * 50000 + 2 * 48000 + 2 * 43000 + 2 * 24000 + 2 * 11500, SDS-PAGE. The enzyme complex consists of a core enzyme of 4 subunits with the composition alpha2beta2gamma2delta2, this complex is able to reduce viologen dyes. The holoenzyme contains in addition an epsilon2 unit that catalyzes the transfer of electrons from a small ferredoxin-like subunit of the core complex to NAD, this unit also catalyzes the transhydrogenase reaction, carries FAD and resembles ferredoxin:NAD(P)+-oxidoreductase Aromatoleum evansii
1.2.1.58 43000
-
2 * 50000 + 2 * 48000 + 2 * 43000 + 2 * 24000 + 2 * 11500, SDS-PAGE. The enzyme complex consists of a core enzyme of 4 subunits with the composition alpha2beta2gamma2delta2, this complex is able to reduce viologen dyes. The holoenzyme contains in addition an epsilon2 unit that catalyzes the transfer of electrons from a small ferredoxin-like subunit of the core complex to NAD, this unit also catalyzes the transhydrogenase reaction, carries FAD and resembles ferredoxin:NAD(P)+-oxidoreductase Aromatoleum evansii
1.2.1.58 48000
-
2 * 50000 + 2 * 48000 + 2 * 43000 + 2 * 24000 + 2 * 11500, SDS-PAGE. The enzyme complex consists of a core enzyme of 4 subunits with the composition alpha2beta2gamma2delta2, this complex is able to reduce viologen dyes. The holoenzyme contains in addition an epsilon2 unit that catalyzes the transfer of electrons from a small ferredoxin-like subunit of the core complex to NAD, this unit also catalyzes the transhydrogenase reaction, carries FAD and resembles ferredoxin:NAD(P)+-oxidoreductase Aromatoleum evansii
1.2.1.58 50000
-
2 * 50000 + 2 * 48000 + 2 * 43000 + 2 * 24000 + 2 * 11500, SDS-PAGE. The enzyme complex consists of a core enzyme of 4 subunits with the composition alpha2beta2gamma2delta2, this complex is able to reduce viologen dyes. The holoenzyme contains in addition an epsilon2 unit that catalyzes the transfer of electrons from a small ferredoxin-like subunit of the core complex to NAD, this unit also catalyzes the transhydrogenase reaction, carries FAD and resembles ferredoxin:NAD(P)+-oxidoreductase Aromatoleum evansii
1.2.1.58 370000
-
gel filtration Aromatoleum evansii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.58 phenylglyoxylate + NAD+ + CoA-SH Aromatoleum evansii enzyme activity is induced during anaerobic growth with Phe, phenylacetate and phenylglyoxylate, but not with benzoate benzoyl-S-CoA + CO2 + NADH
-
?
1.2.1.58 phenylglyoxylate + NAD+ + CoA-SH Aromatoleum evansii enzyme of anaerobic Phe metabolism benzoyl-S-CoA + CO2 + NADH
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.58 Aromatoleum evansii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.58
-
Aromatoleum evansii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.58 5.1
-
NAD+-dependent reaction Aromatoleum evansii
1.2.1.58 7.4
-
benzyl viologen-dependent reaction Aromatoleum evansii

Storage Stability

EC Number Storage Stability Organism
1.2.1.58 6°C, 1 mM phenylglyoxal, half-life: 2 days Aromatoleum evansii
1.2.1.58 6°C, 10% glycerol, half-life: 4 days Aromatoleum evansii
1.2.1.58 6°C, without glycerol or substrate, half-life: 10 h Aromatoleum evansii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.58 2-oxoisovalerate + NAD+ + CoA-SH at 15% of the activity relative to phenylglyoxylate Aromatoleum evansii ?
-
?
1.2.1.58 additional information enzyme catalyzes a NAD(H):viologen dye transhydrogenation Aromatoleum evansii ?
-
?
1.2.1.58 phenylglyoxylate + benzyl viologen + CoA-SH
-
Aromatoleum evansii benzoyl-S-CoA + CO2 + reduced benzyl viologen
-
?
1.2.1.58 phenylglyoxylate + FAD + CoA-SH
-
Aromatoleum evansii benzoyl-S-CoA + CO2 + FADH2
-
?
1.2.1.58 phenylglyoxylate + FMN + CoA-SH
-
Aromatoleum evansii benzoyl-S-CoA + CO2 + FMNH
-
?
1.2.1.58 phenylglyoxylate + methyl viologen + CoA-SH
-
Aromatoleum evansii benzoyl-S-CoA + CO2 + reduced methyl viologen
-
?
1.2.1.58 phenylglyoxylate + NAD+ + CoA-SH reaction is strictly dependent on CoA Aromatoleum evansii benzoyl-S-CoA + CO2 + NADH
-
?
1.2.1.58 phenylglyoxylate + NAD+ + CoA-SH enzyme activity is induced during anaerobic growth with Phe, phenylacetate and phenylglyoxylate, but not with benzoate Aromatoleum evansii benzoyl-S-CoA + CO2 + NADH
-
?
1.2.1.58 phenylglyoxylate + NAD+ + CoA-SH enzyme of anaerobic Phe metabolism Aromatoleum evansii benzoyl-S-CoA + CO2 + NADH
-
?

Subunits

EC Number Subunits Comment Organism
1.2.1.58 decamer 2 * 50000 + 2 * 48000 + 2 * 43000 + 2 * 24000 + 2 * 11500, SDS-PAGE. The enzyme complex consists of a core enzyme of 4 subunits with the composition alpha2beta2gamma2delta2, this complex is able to reduce viologen dyes. The holoenzyme contains in addition an epsilon2 unit that catalyzes the transfer of electrons from a small ferredoxin-like subunit of the core complex to NAD, this unit also catalyzes the transhydrogenase reaction, carries FAD and resembles ferredoxin:NAD(P)+-oxidoreductase Aromatoleum evansii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.1.58 46
-
benzyl viologen
-
Aromatoleum evansii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.58 8
-
-
Aromatoleum evansii

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.58 FAD contains 1.1 mol FAD per mol of enzyme Aromatoleum evansii
1.2.1.58 thiamine diphosphate reaction is strictly dependent on Aromatoleum evansii