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Literature summary extracted from

  • Huang, C.S.; Chang, L.S.; Anderson, M.E.; Meister, A.
    Catalytic and regulatory properties of the heavy subunit of rat kidney gamma-glutamylcysteine synthetase (1993), J. Biol. Chem., 268, 19675-19680.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.2 cDNA for heavy subunit expressed in Escherichia coli Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.2.2 gamma-Glu-2-aminobutanoyl-Gly i.e. ophthalmic acid, inhibits only slightly, but inhibits much more after treatment of the holoenzyme with DTT, the recombinant and isolated heavy subunit enzyme is substantially inhibited without DTT Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.2 0.2
-
 L-Cys L-Cys Rattus norvegicus
6.3.2.2 0.8
-
 L-2-aminobutanoate recombinant heavy subunit Rattus norvegicus
6.3.2.2 1.2
-
 L-Glu L-2-aminobutanoate Rattus norvegicus
6.3.2.2 1.4
-
 L-2-aminobutanoate L-Glu, holoenzyme Rattus norvegicus
6.3.2.2 18.2
-
 L-Glu recombinant heavy subunit Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.2.2 27700
-
 1 * 73000 + 1 * 27700, PAGE in presence of 50 mM DTT Rattus norvegicus
6.3.2.2 73000
-
 1 * 73000 + 1 * 27700, PAGE in presence of 50 mM DTT Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.2 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.2 recombinant enzyme Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.2.2 kidney
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.2 ATP + L-Glu + L-Cys
-
 Rattus norvegicus ADP + phosphate + gamma-L-Glu-L-Cys
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.2.2 dimer 1 * 73000 + 1 * 27700, PAGE in presence of 50 mM DTT Rattus norvegicus
6.3.2.2 More the light subunit has a regulatory function affecting the affinity for Glu and GSH Rattus norvegicus