EC Number | Cloned (Comment) | Organism |
---|---|---|
6.3.2.2 | expression in Escherichia coli, 36-45% amino acid sequence identity with the enzymes from rat, human, Saccharomyces cerevisiae and Schizosaccharomyces pombe | Trypanosoma brucei |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
6.3.2.2 | cystamine | - |
Trypanosoma brucei | |
6.3.2.2 | glutathione | GSH | Trypanosoma brucei |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.2.2 | 0.07 | - |
ATP | - |
Trypanosoma brucei | |
6.3.2.2 | 0.24 | - |
L-Glu | - |
Trypanosoma brucei | |
6.3.2.2 | 0.69 | - |
L-Cys | - |
Trypanosoma brucei | |
6.3.2.2 | 10 | - |
L-2-aminobutanoate | - |
Trypanosoma brucei | |
6.3.2.2 | 10.4 | - |
L-2-aminobutanoate | - |
Trypanosoma brucei |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.2.2 | ATP + L-Glu + L-Cys | Trypanosoma brucei | enzyme catalyzes the first committed step in the biosynthesis of trypanothione, i.e. diglutathionylspermidine | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.3.2.2 | Trypanosoma brucei | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.3.2.2 | - |
Trypanosoma brucei |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.2.2 | ATP + L-Glu + L-2-aminobutanoate | - |
Trypanosoma brucei | ADP + phosphate + gamma-L-Glu-2-aminobutanoate | - |
? | |
6.3.2.2 | ATP + L-Glu + L-Cys | - |
Trypanosoma brucei | ADP + phosphate + gamma-L-Glu-L-Cys | - |
? | |
6.3.2.2 | ATP + L-Glu + L-Cys | enzyme catalyzes the first committed step in the biosynthesis of trypanothione, i.e. diglutathionylspermidine | Trypanosoma brucei | ? | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.2.2 | additional information | - |
additional information | - |
Trypanosoma brucei |