Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hiromasa, Y.; Aso, Y.; Yamashita, S.
    Purification of pyruvate dehydrogenase complex from an extreme thermophile, Bacillus caldolyticus, and its thermal stability (1993), Biosci. Biotechnol. Biochem., 57, 1062-1066.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.104 0.117
-
 pyruvate pH 7.0, 30°C [Bacillus] caldolyticus
1.2.1.104 1.27
-
 2-oxoisovalerate pH 7.0, 30°C [Bacillus] caldolyticus
4.1.1.1 0.117
-
 pyruvate
-
 [Bacillus] caldolyticus
4.1.1.1 1.27
-
 2-oxoisopentanoate
-
 [Bacillus] caldolyticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.1 39800
-
 1 * 39800 + 1 * 41700, the dimeric pyruvate decarboxylase is a component of the multienzyme complex pyruvate dehydrogenase, SDS-PAGE [Bacillus] caldolyticus
4.1.1.1 41700
-
 1 * 39800 + 1 * 41700, the dimeric pyruvate decarboxylase is a component of the multienzyme complex pyruvate dehydrogenase, SDS-PAGE [Bacillus] caldolyticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.1 additional information [Bacillus] caldolyticus pyruvate decarboxylase is one enzyme component of the pyruvate dehydrogenase complex ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.104 [Bacillus] caldolyticus
-
-
-
4.1.1.1 [Bacillus] caldolyticus
-
 pyruvate decarboxylase is one enzyme component of the pyruvate dehydrogenase complex
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.104 purification of pyruvate dehydrogenase complex including pyruvate decarboxylase E1 [Bacillus] caldolyticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.1 additional information
-
-
 [Bacillus] caldolyticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.104 2-oxoisovalerate + CoA + NAD+
-
 [Bacillus] caldolyticus isobutanoyl-CoA + CO2 + NADH
-
 ?
1.2.1.104 pyruvate + CoA + NAD+
-
 [Bacillus] caldolyticus acetyl-CoA + CO2 + NADH
-
 ?
4.1.1.1 2-Oxoisopentanoate
-
 [Bacillus] caldolyticus ?
-
 ?
4.1.1.1 additional information pyruvate decarboxylase is one enzyme component of the pyruvate dehydrogenase complex [Bacillus] caldolyticus ?
-
 ?
4.1.1.1 pyruvate
-
 [Bacillus] caldolyticus acetaldehyde + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.1 dimer 1 * 39800 + 1 * 41700, the dimeric pyruvate decarboxylase is a component of the multienzyme complex pyruvate dehydrogenase, SDS-PAGE [Bacillus] caldolyticus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.104 65
-
 half-life 30 min, pyruvate decarboxylase subunit E1 [Bacillus] caldolyticus
4.1.1.1 65
-
 30 min, 50% loss of activity [Bacillus] caldolyticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.104 7.5 8
-
 [Bacillus] caldolyticus
4.1.1.1 7.5 8
-
 [Bacillus] caldolyticus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.2.1.104 5.7 10.2 pyruvate decarboxylase subunit E1 [Bacillus] caldolyticus