EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.1.104 | 0.117 | - |
pyruvate | pH 7.0, 30°C | [Bacillus] caldolyticus | |
1.2.1.104 | 1.27 | - |
2-oxoisovalerate | pH 7.0, 30°C | [Bacillus] caldolyticus | |
4.1.1.1 | 0.117 | - |
pyruvate | - |
[Bacillus] caldolyticus | |
4.1.1.1 | 1.27 | - |
2-oxoisopentanoate | - |
[Bacillus] caldolyticus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.1.1.1 | 39800 | - |
1 * 39800 + 1 * 41700, the dimeric pyruvate decarboxylase is a component of the multienzyme complex pyruvate dehydrogenase, SDS-PAGE | [Bacillus] caldolyticus |
4.1.1.1 | 41700 | - |
1 * 39800 + 1 * 41700, the dimeric pyruvate decarboxylase is a component of the multienzyme complex pyruvate dehydrogenase, SDS-PAGE | [Bacillus] caldolyticus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.1.1 | additional information | [Bacillus] caldolyticus | pyruvate decarboxylase is one enzyme component of the pyruvate dehydrogenase complex | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.1.104 | [Bacillus] caldolyticus | - |
- |
- |
4.1.1.1 | [Bacillus] caldolyticus | - |
pyruvate decarboxylase is one enzyme component of the pyruvate dehydrogenase complex | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.2.1.104 | purification of pyruvate dehydrogenase complex including pyruvate decarboxylase E1 | [Bacillus] caldolyticus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.1.1.1 | additional information | - |
- |
[Bacillus] caldolyticus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.104 | 2-oxoisovalerate + CoA + NAD+ | - |
[Bacillus] caldolyticus | isobutanoyl-CoA + CO2 + NADH | - |
? | |
1.2.1.104 | pyruvate + CoA + NAD+ | - |
[Bacillus] caldolyticus | acetyl-CoA + CO2 + NADH | - |
? | |
4.1.1.1 | 2-Oxoisopentanoate | - |
[Bacillus] caldolyticus | ? | - |
? | |
4.1.1.1 | additional information | pyruvate decarboxylase is one enzyme component of the pyruvate dehydrogenase complex | [Bacillus] caldolyticus | ? | - |
? | |
4.1.1.1 | pyruvate | - |
[Bacillus] caldolyticus | acetaldehyde + CO2 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.1.1.1 | dimer | 1 * 39800 + 1 * 41700, the dimeric pyruvate decarboxylase is a component of the multienzyme complex pyruvate dehydrogenase, SDS-PAGE | [Bacillus] caldolyticus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.2.1.104 | 65 | - |
half-life 30 min, pyruvate decarboxylase subunit E1 | [Bacillus] caldolyticus |
4.1.1.1 | 65 | - |
30 min, 50% loss of activity | [Bacillus] caldolyticus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.2.1.104 | 7.5 | 8 | - |
[Bacillus] caldolyticus |
4.1.1.1 | 7.5 | 8 | - |
[Bacillus] caldolyticus |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.2.1.104 | 5.7 | 10.2 | pyruvate decarboxylase subunit E1 | [Bacillus] caldolyticus |