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Literature summary extracted from

  • Sriram, R.; Ali-Osman, F.
    Purification and biochemical characterization of gamma-glutamylcysteine synthetase from a human malignant astrocytoma cell line (1993), Biochem. Mol. Biol. Int., 30, 1053-1060.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.2 0.03
-
 L-Glu
-
 Homo sapiens
6.3.2.2 0.14
-
 L-2-aminobutanoate
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.2.2 32000
-
 x * 72000 + x * 32000, denaturing SDS-PAGE Homo sapiens
6.3.2.2 72000
-
 x * 72000 + x * 32000, denaturing SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.2 ATP + L-Glu + L-Cys Homo sapiens key regulatory enzyme in glutathione biosynthesis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.2 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.2
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.2.2 astrocytoma cell line malignant Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.2.2 28.75
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.2 ATP + L-Glu + L-2-aminobutanoate
-
 Homo sapiens ADP + phosphate + gamma-L-Glu-2-aminobutanoate
-
 ?
6.3.2.2 ATP + L-Glu + L-Cys
-
 Homo sapiens ADP + phosphate + gamma-L-Glu-L-Cys
-
 ?
6.3.2.2 ATP + L-Glu + L-Cys key regulatory enzyme in glutathione biosynthesis Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.2.2 ? x * 72000 + x * 32000, denaturing SDS-PAGE Homo sapiens