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Literature summary extracted from
- Characterization of recombinant fungal phytase (phyA) expressed in tobacco leaves (1999), Biochem. Biophys. Res. Commun., 264, 201-206.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.3.8 | expression in Nicotiana tabacum leaves | Aspergillus ficuum |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.8 | myo-inositol hexasulfate | - |
Aspergillus ficuum |  |
| 3.1.3.8 | Phenylglyoxal | - |
Aspergillus ficuum | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.3.8 | 0.027 | - |
myo-inositol hexakisphosphate | native fungal enzyme | Aspergillus ficuum | |
| 3.1.3.8 | 0.065 | - |
myo-inositol hexakisphosphate | recombinant enzyme | Aspergillus ficuum | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.3.8 | Aspergillus ficuum | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.1.3.8 | side-chain modification | glycoprotein | Aspergillus ficuum |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.3.8 | recombinant enzyme | Aspergillus ficuum |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.8 | additional information | - |
- |
Aspergillus ficuum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.3.8 | myo-inositol hexakisphosphate + H2O | - |
Aspergillus ficuum | 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.8 | 58 | - |
recombinant and native enzyme | Aspergillus ficuum |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.3.8 | 348 | - |
myo-inositol hexakisphosphate | native fungal enzyme | Aspergillus ficuum | |
| 3.1.3.8 | 360 | - |
myo-inositol hexakisphosphate | recombinant enzyme | Aspergillus ficuum | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.8 | 4 | - |
recombinant enzyme | Aspergillus ficuum |
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Release 2023.1 (January 2023)
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