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Literature summary extracted from

  • Pasamontes, L.; Haiker, M.; Wyss, M.; Tessier, M.; van Loon, A.P.G.M.
    Gene cloning, purification and characterization of a heat-stable phytase from the fungus Aspergillus fumigatus (1997), Appl. Environ. Microbiol., 63, 1696-1700.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.8 overexpression in Aspergillus niger Aspergillus fumigatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.8 48270
-
 x * 48270, calculation from nucleotide sequence Aspergillus fumigatus
3.1.3.8 60000
-
 x * 60000, SDS-PAGE Aspergillus fumigatus

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.8 Aspergillus fumigatus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.3.8 side-chain modification glycoprotein Aspergillus fumigatus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.8
-
 Aspergillus fumigatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.8 4-nitrophenyl phosphate + H2O
-
 Aspergillus fumigatus 4-nitrophenol + phosphate
-
 ?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
 Aspergillus fumigatus 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.8 ? x * 60000, SDS-PAGE Aspergillus fumigatus
3.1.3.8 ? x * 48270, calculation from nucleotide sequence Aspergillus fumigatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.8 90
-
 20 min, no discernible effect on secondary structure Aspergillus fumigatus
3.1.3.8 100
-
 half-life is close to 70 min Aspergillus fumigatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.8 5
-
 hydrolysis of 4-nitrophenyl phosphate Aspergillus fumigatus
3.1.3.8 6 6.5 hydrolysis of myo-inositol hexakisphosphate Aspergillus fumigatus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.8 3 5.5 pH 3.0: about 55% of maximal activity, pH 5.5: about 50% of maximal activity, hydrolysis of 4-nitrophenyl phosphate Aspergillus fumigatus