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Literature summary extracted from

  • Rodriguez, E.; Mullaney, E.J.; Lei, X.G.
    Expression of the Aspergillus fumigatus phytase gene in Pichia pastoris and characterization of the recombinant enzyme (2000), Biochem. Biophys. Res. Commun., 268, 373-378.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.8 expression in Pichia pastoris Aspergillus fumigatus
3.1.3.26 expression in Pichia pastoris Aspergillus fumigatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.8 1.38
-
 4-nitrophenyl phosphate
-
 Aspergillus fumigatus
3.1.3.8 1.38
-
 4-nitrophenyl phosphate pH 2.5 Aspergillus fumigatus
3.1.3.8 2.84
-
 myo-inositol hexakisphosphate
-
 Aspergillus fumigatus
3.1.3.8 2.84
-
 myo-inositol hexakisphosphate pH 5.5 Aspergillus fumigatus
3.1.3.26 1.38
-
 4-nitrophenyl phosphate pH 2.5 Aspergillus fumigatus
3.1.3.26 2.84
-
 myo-inositol hexakisphosphate pH 5.5 Aspergillus fumigatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.8 49000
-
 x * 49000, SDS-PAGE after deglycosylation Aspergillus fumigatus
3.1.3.8 76000
-
 1 * 76000, SDS-PAGE Aspergillus fumigatus
3.1.3.8 76000
-
 x * 76000, SDS-PAGE before deglycosylation Aspergillus fumigatus
3.1.3.8 92000
-
 nondenaturing PAGE Aspergillus fumigatus
3.1.3.8 92000
-
 non-denaturing PAGE Aspergillus fumigatus
3.1.3.26 76000
-
 1 * 76000, SDS-PAGE Aspergillus fumigatus
3.1.3.26 92000
-
 non-denaturing PAGE Aspergillus fumigatus

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.8 Aspergillus fumigatus
-
-
-
3.1.3.8 Aspergillus fumigatus
-
 The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
3.1.3.26 Aspergillus fumigatus
-
 The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.3.8 glycoprotein 36% glycosylation Aspergillus fumigatus
3.1.3.8 side-chain modification 36% glycosylation Aspergillus fumigatus
3.1.3.26 glycoprotein 36% glycosylation Aspergillus fumigatus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.8 recombinant enzyme Aspergillus fumigatus
3.1.3.26 recombinant enzyme Aspergillus fumigatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.8 4-nitrophenyl phosphate + H2O
-
 Aspergillus fumigatus 4-nitrophenol + phosphate
-
 ?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
 Aspergillus fumigatus ? + phosphate
-
 ?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
 Aspergillus fumigatus 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
 ?
3.1.3.26 4-nitrophenyl phosphate + H2O
-
 Aspergillus fumigatus 4-nitrophenol + phosphate
-
 ?
3.1.3.26 myo-inositol hexakisphosphate + H2O
-
 Aspergillus fumigatus ? + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.8 ? x * 76000, SDS-PAGE before deglycosylation Aspergillus fumigatus
3.1.3.8 ? x * 49000, SDS-PAGE after deglycosylation Aspergillus fumigatus
3.1.3.8 monomer 1 * 76000, SDS-PAGE Aspergillus fumigatus
3.1.3.26 monomer 1 * 76000, SDS-PAGE Aspergillus fumigatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.8 60
-
-
 Aspergillus fumigatus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.3.8 37 80 about 45% of maximal activity at 37°C and at 80°C Aspergillus fumigatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.8 additional information
-
 better thermostability at 65°C and at 90°C when sodium acetate is used as the buffer, pH 5.5, than that of sodium citrate at either low, 10 mM or high, 0.2 M, concentration Aspergillus fumigatus
3.1.3.8 37
-
 20 min, less than 5% of activity of enzyme in 0.01 M sodium citrate buffer, about 5% loss of activity in 0.2 M sodium citrate buffer, about 10% loss of activity in 0.2 M sodium acetate buffer, about 15% loss of activity in 0.01 sodium acetate bugffer Aspergillus fumigatus
3.1.3.8 65
-
 20 min, about 35% loss of activity in 0.01 M sodium acetate buffer, about 40% loss of activity in 0.2 M acetate buffer, about 45% loss of activity in 0.01 M sodium citrate buffer, about 65% loss of activity in 0.2 M sodium citrate buffer Aspergillus fumigatus
3.1.3.8 90
-
 20 min, about 35% loss of activity in 0.01 M sodium acetate buffer, about 40% loss of activity in 0.2 M acetate buffer, about 45% loss of activity in 0.01 M sodium citrate buffer, about 65% loss of activity in 0.2 M sodium citrate buffer Aspergillus fumigatus
3.1.3.26 37
-
 20 min, less than 5% of activity of enzyme in 0.01 M sodium citrate buffer, about 5% loss of activity in 0.2 M sodium citrate buffer, about 10% loss of activity in 0.2 M sodium acetate buffer, about 15% loss of activity in 0.01 sodium acetate bugffer Aspergillus fumigatus
3.1.3.26 65
-
 20 min, about 35% loss of activity in 0.01 M sodium acetate buffer, about 40% loss of activity in 0.2 M acetate buffer, about 45% loss of activity in 0.01 M sodium citrate buffer, about 65% loss of activity in 0.2 M sodium citrate buffer Aspergillus fumigatus
3.1.3.26 90
-
 20 min, about 35% loss of activity in 0.01 M sodium acetate buffer, about 40% loss of activity in 0.2 M acetate buffer, about 45% loss of activity in 0.01 M sodium citrate buffer, about 65% loss of activity in 0.2 M sodium citrate buffer Aspergillus fumigatus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.8 99
-
 4-nitrophenyl phosphate
-
 Aspergillus fumigatus
3.1.3.8 114
-
 myo-inositol hexakisphosphate
-
 Aspergillus fumigatus
3.1.3.8 357500
-
 4-nitrophenyl phosphate pH 2.5 Aspergillus fumigatus
3.1.3.8 358000
-
 4-nitrophenyl phosphate pH 2.5 Aspergillus fumigatus
3.1.3.8 411700
-
 myo-inositol hexakisphosphate pH 5.5 Aspergillus fumigatus
3.1.3.8 412000
-
 myo-inositol hexakisphosphate pH 5.5 Aspergillus fumigatus
3.1.3.26 357500
-
 4-nitrophenyl phosphate pH 2.5 Aspergillus fumigatus
3.1.3.26 358000
-
 4-nitrophenyl phosphate pH 2.5 Aspergillus fumigatus
3.1.3.26 411700
-
 myo-inositol hexakisphosphate pH 5.5 Aspergillus fumigatus
3.1.3.26 412000
-
 myo-inositol hexakisphosphate pH 5.5 Aspergillus fumigatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.8 6
-
-
 Aspergillus fumigatus
3.1.3.8 6
-
 and a second lower optimum at pH 4.0, reaction with phytate Aspergillus fumigatus
3.1.3.26 6
-
 and a second lower optimum at pH 4.0, reaction with phytate Aspergillus fumigatus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.8 3.5 7 pH 3.5: about 35% of maximal activity, pH 7.0: about 70% of maximal activity Aspergillus fumigatus
3.1.3.8 3.5 7 pH 3.5: about 35% of maximal activity, pH 7.0: about 70% of maximal activity, reaction with phytate Aspergillus fumigatus
3.1.3.26 3.5 7 pH 3.5: about 35% of maximal activity, pH 7.0: about 70% of maximal activity, reaction with phytate Aspergillus fumigatus