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Literature summary extracted from

  • Greiner, R.; Haller, E.; Konietzny, U.; Jany, K.D.
    Purification and characterization of a phytase from Klebsiella terrigena (1997), Arch. Biochem. Biophys., 341, 201-206.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.8 Ca2+ 0.1-1 mM, slight inhibition Raoultella terrigena
3.1.3.8 Cu2+ 0.1-1 mM, strong inhibition Raoultella terrigena
3.1.3.8 F-
-
 Raoultella terrigena
3.1.3.8 Mn2+ 0.1-1 mM, slight inhibition Raoultella terrigena
3.1.3.8 molybdate
-
 Raoultella terrigena
3.1.3.8 phosphate
-
 Raoultella terrigena
3.1.3.8 vanadate
-
 Raoultella terrigena
3.1.3.8 Zn2+ 0.1-1 mM, strong inhibition Raoultella terrigena

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.8 0.3
-
 myo-inositol hexakisphosphate
-
 Raoultella terrigena
3.1.3.8 6
-
 NADP+
-
 Raoultella terrigena
3.1.3.8 18
-
 fructose 1,6-diphosphate
-
 Raoultella terrigena
3.1.3.8 20
-
 glucose 6-phosphate
-
 Raoultella terrigena
3.1.3.8 55
-
 ATP
-
 Raoultella terrigena
3.1.3.8 56
-
 fructose 6-phosphate
-
 Raoultella terrigena
3.1.3.8 78
-
 diphosphate
-
 Raoultella terrigena
3.1.3.8 90
-
 ADP
-
 Raoultella terrigena
3.1.3.8 95
-
 AMP
-
 Raoultella terrigena
3.1.3.8 110
-
 Glycerol 2-phosphate
-
 Raoultella terrigena
3.1.3.8 120
-
 4-nitrophenyl phosphate
-
 Raoultella terrigena
3.1.3.8 230
-
 2-naphthyl phosphate
-
 Raoultella terrigena
3.1.3.8 250
-
 1-naphthyl phosphate
-
 Raoultella terrigena

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.8 40000
-
 gel filtration Raoultella terrigena

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.8 additional information Raoultella terrigena the enzyme is inducible under carbon limitation in the presence of myo-inositol hexakisphosphate ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.8 Raoultella terrigena
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.8
-
 Raoultella terrigena

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.8 205
-
-
 Raoultella terrigena

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.8 1-naphthyl phosphate + H2O
-
 Raoultella terrigena 1-naphthol + phosphate
-
 ?
3.1.3.8 2-naphthyl phosphate + H2O
-
 Raoultella terrigena 2-naphthol + phosphate
-
 ?
3.1.3.8 4-nitrophenyl phosphate + H2O
-
 Raoultella terrigena 4-nitrophenol + phosphate
-
 ?
3.1.3.8 ADP + H2O
-
 Raoultella terrigena ?
-
 ?
3.1.3.8 AMP + H2O
-
 Raoultella terrigena adenosine + phosphate
-
 ?
3.1.3.8 ATP + H2O
-
 Raoultella terrigena ?
-
 ?
3.1.3.8 D-fructose 6-phosphate + H2O
-
 Raoultella terrigena D-fructose + phosphate
-
 ?
3.1.3.8 diphosphate + H2O
-
 Raoultella terrigena 2 phosphate
-
 ?
3.1.3.8 fructose 1,6-diphosphate + H2O
-
 Raoultella terrigena ?
-
 ?
3.1.3.8 Glucose 6-phosphate + H2O
-
 Raoultella terrigena Glucose + phosphate
-
 ?
3.1.3.8 glycerol 2-phosphate + H2O
-
 Raoultella terrigena glycerol + phosphate
-
 ?
3.1.3.8 GTP + H2O
-
 Raoultella terrigena ?
-
 ?
3.1.3.8 additional information the enzyme is inducible under carbon limitation in the presence of myo-inositol hexakisphosphate Raoultella terrigena ?
-
 ?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
 Raoultella terrigena 1-L-myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
 ?
3.1.3.8 NADP+ + H2O
-
 Raoultella terrigena ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.8 monomer x * 40000, SDS-PAGE Raoultella terrigena

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.8 58
-
-
 Raoultella terrigena

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.8 40
-
 1 h, stable up to Raoultella terrigena
3.1.3.8 50
-
 1 h, 20% loss of activity Raoultella terrigena
3.1.3.8 60
-
 1 h, complete inactivation Raoultella terrigena

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.8 2
-
 glucose 6-phosphate
-
 Raoultella terrigena
3.1.3.8 2
-
 1-naphthyl phosphate
-
 Raoultella terrigena
3.1.3.8 4
-
 Glycerol 2-phosphate
-
 Raoultella terrigena
3.1.3.8 6
-
 ATP
-
 Raoultella terrigena
3.1.3.8 6
-
 fructose 6-phosphate
-
 Raoultella terrigena
3.1.3.8 6
-
 2-naphthyl phosphate
-
 Raoultella terrigena
3.1.3.8 8
-
 AMP
-
 Raoultella terrigena
3.1.3.8 9
-
 ADP
-
 Raoultella terrigena
3.1.3.8 10
-
 fructose 1,6-diphosphate
-
 Raoultella terrigena
3.1.3.8 11
-
 diphosphate
-
 Raoultella terrigena
3.1.3.8 20
-
 4-nitrophenyl phosphate
-
 Raoultella terrigena
3.1.3.8 46
-
 NADP+
-
 Raoultella terrigena
3.1.3.8 180
-
 myo-inositol hexakisphosphate
-
 Raoultella terrigena

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.8 5
-
-
 Raoultella terrigena

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.3.8 1
-
 4°C, 24 h, 85% loss of activity Raoultella terrigena
3.1.3.8 2
-
 4°C, 24 h, 45% loss of activity Raoultella terrigena
3.1.3.8 3 9 4°C, 10 days, no loss of activity Raoultella terrigena
3.1.3.8 5
-
 30-50°C, no loss of activity Raoultella terrigena