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Literature summary extracted from

  • Roth, S.C.; Harkness, D.R.; Isaacks, R.E.
    Studies on avian erythrocyte metabolism: purification and properties of myo-inositol 1-phosphatase from chick erythrocytes (1981), Arch. Biochem. Biophys., 210, 465-473.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.25 Ca2+
-
 Gallus gallus
3.1.3.25 EDTA
-
 Gallus gallus
3.1.3.25 HgCl2
-
 Gallus gallus
3.1.3.25 myo-inositol 1,3,4,5,6 pentakis(dihydrogen phosphate)
-
 Gallus gallus
3.1.3.25 myo-Inositol tetrakis(dihydrogen phosphate)
-
 Gallus gallus
3.1.3.25 NaF 90% inhibition by 10 mM Gallus gallus
3.1.3.25 p-hydroxymercuribenzoate
-
 Gallus gallus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.25 0.086
-
 DL-myo-inositol 1-phosphate
-
 Gallus gallus
3.1.3.25 0.286
-
 inositol 2-phosphate
-
 Gallus gallus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.25 Co2+
-
 Gallus gallus
3.1.3.25 Fe2+
-
 Gallus gallus
3.1.3.25 Mg2+ dependent on Gallus gallus
3.1.3.25 Mn2+ stimulates Gallus gallus
3.1.3.25 additional information absolute requirement for divalent cations, Mg2+ greatest activity Gallus gallus
3.1.3.25 Zn2+
-
 Gallus gallus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.25 60000
-
 gel filtration Gallus gallus

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.25 Gallus gallus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.25
-
 Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.25 erythrocyte
-
 Gallus gallus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.25 additional information
-
-
 Gallus gallus

Storage Stability

EC Number Storage Stability Organism
3.1.3.25 at 4°C stable for 1 week, after that time 30% loss of activity per week Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.25 2'-AMP + H2O
-
 Gallus gallus adenosine + phosphate
-
 ?
3.1.3.25 2'-CMP + H2O
-
 Gallus gallus cytidine + phosphate
-
 ?
3.1.3.25 2'-GMP + H2O
-
 Gallus gallus guanosine + phosphate
-
 ?
3.1.3.25 2'-UMP + H2O
-
 Gallus gallus uridine + phosphate
-
 ?
3.1.3.25 2,3-diphosphoglycerate + H2O
-
 Gallus gallus ?
-
 ?
3.1.3.25 2-phosphoglycerate + H2O
-
 Gallus gallus ?
-
 ?
3.1.3.25 2-phosphoglycolate + H2O
-
 Gallus gallus ?
-
 ?
3.1.3.25 3'-AMP + H2O
-
 Gallus gallus adenosine + phosphate
-
 ?
3.1.3.25 3'-CMP + H2O
-
 Gallus gallus cytidine + phosphate
-
 ?
3.1.3.25 3'-GMP + H2O
-
 Gallus gallus guanosine + phosphate
-
 ?
3.1.3.25 3'-UMP + H2O
-
 Gallus gallus uridine + phosphate
-
 ?
3.1.3.25 3-phosphoglycerate + H2O
-
 Gallus gallus ?
-
 ?
3.1.3.25 5'-CMP + H2O
-
 Gallus gallus cytidine + phosphate
-
 ?
3.1.3.25 5'-dAMP + H2O
-
 Gallus gallus adenosine + phosphate
-
 ?
3.1.3.25 5'-GMP + H2O
-
 Gallus gallus guanosine + phosphate
-
 ?
3.1.3.25 5'-UMP + H2O
-
 Gallus gallus uridine + phosphate
-
 ?
3.1.3.25 D-glucose 6-phosphate + H2O
-
 Gallus gallus ?
-
 ?
3.1.3.25 DL-myo-inositol 1-phosphate + H2O
-
 Gallus gallus DL-myo-inositol + phosphate
-
 ?
3.1.3.25 glycerol 2-phosphate + H2O
-
 Gallus gallus glycerol + phosphate
-
 ?
3.1.3.25 inosine 5'-phosphate + H2O
-
 Gallus gallus ?
-
 ?
3.1.3.25 inositol 2-phosphate + H2O
-
 Gallus gallus ?
-
 ?
3.1.3.25 L-glycerol 1-phosphate + H2O
-
 Gallus gallus glycerol + phosphate
-
 ?
3.1.3.25 additional information small activity: 5-ribonucleotides Gallus gallus ?
-
 ?
3.1.3.25 purine nucleotides + H2O
-
 Gallus gallus ?
-
 ?
3.1.3.25 pyrimidine ribonucleotides + H2O
-
 Gallus gallus ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.25 60
-
 no loss of activity after 3 h, dialyzed hemolysates Gallus gallus
3.1.3.25 70
-
 no loss of activity after 30 min Gallus gallus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.25 7
-
-
 Gallus gallus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.25 5.5 9
-
 Gallus gallus
3.1.3.25 6.5 7.2
-
 Gallus gallus