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Literature summary extracted from

  • Gumber, S.C.; Loewus, M.W.; Loewus, F.A.
    Further studies on myo-inositol-1-phosphatase from the pollen of Lilium longiflorum Thunb. (1984), Plant Physiol., 76, 40-44.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.25 (NH4)2SO4 30% inhibition at 10 mM Lilium longiflorum
3.1.3.25 2-mercaptoethanol
-
 Lilium longiflorum
3.1.3.25 dithiothreitol
-
 Lilium longiflorum
3.1.3.25 F-
-
 Lilium longiflorum
3.1.3.25 HgCl2 95% inhibition at 1 mM Lilium longiflorum
3.1.3.25 iodoacetate 30% inhibition at 10 mM Lilium longiflorum
3.1.3.25 molybdate
-
 Lilium longiflorum
3.1.3.25 N-ethylmaleimide substrate and Mg2+ protect Lilium longiflorum
3.1.3.25 Na2MoO4 30% inhibition at 10 mM Lilium longiflorum
3.1.3.25 NaF 45% inhibition at 1 mM Lilium longiflorum
3.1.3.25 p-hydroxymercuribenzoate
-
 Lilium longiflorum
3.1.3.25 SO42- hydrolyzed very slowly Lilium longiflorum
3.1.3.25 sulfhydryl reagents
-
 Lilium longiflorum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.25 0.044
-
 1-L-chiro-inositol 3-phosphate
-
 Lilium longiflorum
3.1.3.25 0.82
-
 D-mannitol-6-phosphate
-
 Lilium longiflorum
3.1.3.25 2
-
 D-glucitol-6-phosphate
-
 Lilium longiflorum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.25 29000
-
 2 * 29000, SDS-PAGE Lilium longiflorum

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.25 Lilium longiflorum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.25
-
 Lilium longiflorum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.25 pollen
-
 Lilium longiflorum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.25 additional information
-
-
 Lilium longiflorum

Storage Stability

EC Number Storage Stability Organism
3.1.3.25 4°C, 2-mercaptoethanol or DTT, complete loss of activity after 4 days, no loss of activity then stored in 50 mM Tris-acetate, pH 8 for 1 month Lilium longiflorum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.25 1-L-chiro-inositol 3-phosphate
-
 Lilium longiflorum 1-L-chiro-inositol + phosphate
-
 ?
3.1.3.25 D-glucitol-6-phosphate + H2O hydrolyzed very slowly Lilium longiflorum D-glucitol + phosphate
-
 ?
3.1.3.25 D-mannitol-6-phosphate + H2O hydrolyzed very slowly Lilium longiflorum D-mannitol + phosphate
-
 ?
3.1.3.25 additional information not: inorganic phosphate, myo-inositol pentaphosphate Lilium longiflorum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.25 dimer 2 * 29000, SDS-PAGE Lilium longiflorum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.25 37
-
-
 Lilium longiflorum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.25 23
-
 stable overnight, enzyme from Sepharose step Lilium longiflorum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.3.25 3
-
 stable at low pH values, inactivated below pH 3 Lilium longiflorum