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Literature summary extracted from

  • Shevchik, V.E.; Condemine, G.; Hugouvieux-Cotte-Pattat, N.; Robert-Baudouy, J.
    Characterization of pectin methylesterase B, an outer membrane lipoprotein of Erwinia chrysanthemi 3937 (1996), Mol. Microbiol., 19, 455-466.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.11 overexpression in Escherichia coli Dickeya chrysanthemi

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.11 SDS 0.1% complete inactivation Dickeya chrysanthemi

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.11 pectin + H2O Dickeya chrysanthemi the enzyme is required for the growth of bacteria on oligomeric substrates, probably involved in the degradation of methylated oligogalacturonides present in the periplasm of the bacteria ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.11 Dickeya chrysanthemi
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.11 side-chain modification lipoprotein Dickeya chrysanthemi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.11 methylated oligogalacturonides + H2O
-
 Dickeya chrysanthemi ?
-
 ?
3.1.1.11 pectin + H2O
-
 Dickeya chrysanthemi methanol + pectate
-
 ?
3.1.1.11 pectin + H2O the enzyme is required for the growth of bacteria on oligomeric substrates, probably involved in the degradation of methylated oligogalacturonides present in the periplasm of the bacteria Dickeya chrysanthemi ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.11 40
-
-
 Dickeya chrysanthemi

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.11 40
-
 5 h, stable Dickeya chrysanthemi
3.1.1.11 50
-
 half-life: 10 min Dickeya chrysanthemi
3.1.1.11 60
-
 complete loss of activity after 5 min Dickeya chrysanthemi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.11 7.5
-
-
 Dickeya chrysanthemi