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Literature summary extracted from
- Immobilization of pectin esterase from tomatoes and Aspergillus foetidus on various supports (1985), Collect. Czech. Chem. Commun., 50, 2021-2027.
No PubMed abstract available
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.1.1.11 | increased temperature stability after immobilization | Solanum lycopersicum |
| 3.1.1.11 | increased temperature stability after immobilization | Aspergillus foetidus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.11 | Aspergillus foetidus | - |
- |
- |
| 3.1.1.11 | Solanum lycopersicum | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.11 | additional information | immobilized enzymes show about 7.5% of the activity of the free enzyme | Solanum lycopersicum | ? | - |
? |  |
| 3.1.1.11 | additional information | enzyme immobilized on CNBr-Sepharose 4B show about 11.5% of the activity of the free enzyme | Aspergillus foetidus | ? | - |
? | |
| 3.1.1.11 | additional information | enzyme immobilized on polyethylene terephthalate shows 23.1% of the activity of the free enzyme | Aspergillus foetidus | ? | - |
? | |
| 3.1.1.11 | additional information | the immobilized enzyme, unlike the free pectin esterase, does not act on pectin showing a higher esterification degree | Solanum lycopersicum | ? | - |
? | |
| 3.1.1.11 | additional information | the immobilized enzyme, unlike the free pectin esterase, does not act on pectin showing a higher esterification degree | Aspergillus foetidus | ? | - |
? | |
| 3.1.1.11 | pectin + H2O | - |
Solanum lycopersicum | methanol + pectate | - |
? | |
| 3.1.1.11 | pectin + H2O | - |
Aspergillus foetidus | methanol + pectate | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.11 | 40 | - |
soluble enzyme | Aspergillus foetidus |
| 3.1.1.11 | 50 | - |
soluble enzyme | Solanum lycopersicum |
| 3.1.1.11 | 50 | - |
CNBr-Sepharose 4B immobilized enzyme | Aspergillus foetidus |
| 3.1.1.11 | 52 | - |
enzyme immobilized on polyethylene terephthalate | Aspergillus foetidus |
| 3.1.1.11 | 61 | - |
enzyme immobilized on polyethylene terephthalate | Solanum lycopersicum |
| 3.1.1.11 | 62 | - |
enzyme immobilized on pectin esterase | Solanum lycopersicum |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.11 | 60 | - |
20 min, about 50% loss of activity of the soluble enzyme, about 20% loss of activity of the immobilized enzyme | Solanum lycopersicum |
| 3.1.1.11 | 65 | - |
20 min, complete loss of activity of the soluble enzyme, about 50% loss of activity of the immobilized enzyme | Solanum lycopersicum |
| 3.1.1.11 | 65 | - |
20 min, about 75% loss of activity of the soluble enzyme, about 20% loss of activity of the immobilized enzyme | Aspergillus foetidus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.11 | 4.6 | - |
enzyme immobilized on CNBr-Sepharose 4B or polyethylenen terephthalate | Aspergillus foetidus |
| 3.1.1.11 | 4.8 | - |
soluble enzyme | Aspergillus foetidus |
| 3.1.1.11 | 7.5 | - |
soluble and immobilized enzyme | Solanum lycopersicum |
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Release 2023.1 (January 2023)
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