BRENDA - Enzyme Database

Glutathione biosynthesis: gamma-Glutamylcysteine synthetase from rat kidney

Seelig, G.F.; Meister, A.; Methods Enzymol. 113, 379-390 (1985) View publication on PubMed

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
6.3.2.2
treatment with 50 mM DTT for more than 1 h at 4°C, leads to some loss of activity, which is partially reversible. Further treatment leads to irreversible loss of activity
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
6.3.2.2
beta-Methylglutamate
-
Rattus norvegicus
6.3.2.2
buthionine sulfoximine
-
Rattus norvegicus
6.3.2.2
cystamine
completely reversible by DTT
Rattus norvegicus
6.3.2.2
D-3-amino-1-chloro-2-pentanone
highly potent irreversible inactivator
Rattus norvegicus
6.3.2.2
D-Glu
-
Rattus norvegicus
6.3.2.2
gamma-Methylglutamate
-
Rattus norvegicus
6.3.2.2
glutathione
-
Rattus norvegicus
6.3.2.2
L-2-Amino-4-oxo-5-chloropentanoate
inactivation requires very low concentration, 0.003-0.006 mM, of Mg2+ or certain other divalent cations, L-Glu, but not D-Glu protects competitively against inactivation, protection is increased in the presence of ATP or ADP
Rattus norvegicus
6.3.2.2
L-3-Amino-1-chloro-2-pentanone
highly potent irreversible inactivator
Rattus norvegicus
6.3.2.2
methionine sulfoximine
-
Rattus norvegicus
6.3.2.2
Prothionine sulfoximine
-
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.3.2.2
0.2
-
L-Cys
ATP, holoenzyme and recombinant heavy subunit
Rattus norvegicus
6.3.2.2
0.3
-
L-Cys
-
Rattus norvegicus
6.3.2.2
1
-
L-2-aminobutanoate
-
Rattus norvegicus
6.3.2.2
1.6
-
L-Glu
-
Rattus norvegicus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.3.2.2
104000
-
-
Rattus norvegicus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
6.3.2.2
ATP + L-Glu + L-Cys
Rattus norvegicus
glutathione biosynthesis
?
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
6.3.2.2
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
6.3.2.2
-
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
6.3.2.2
kidney
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.3.2.2
additional information
-
-
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
6.3.2.2
ATP + alpha-methylglutamate + L-Cys
i.e. 2-amino-2-methylpentanedioate
1122
Rattus norvegicus
ADP + phosphate + alpha-methylglutamyl-L-Cys
-
-
-
?
6.3.2.2
ATP + beta-aminoglutarate + L-Cys
i.e. 3-aminopentanedioate
1122
Rattus norvegicus
ADP + phosphate + beta-aminoglutaryl-L-Cys
-
-
-
?
6.3.2.2
ATP + beta-methylglutamate + L-Cys
i.e. 2-amino-3-methylpentanedioate
1122
Rattus norvegicus
ADP + phosphate + beta-methylglutamyl-L-Cys
-
-
-
?
6.3.2.2
ATP + D-Glu + L-Cys
-
1122
Rattus norvegicus
ADP + phosphate + D-Glu-L-Cys
-
1122
Rattus norvegicus
?
6.3.2.2
ATP + L-Glu + beta-chloro-L-Ala
-
1122
Rattus norvegicus
ADP + phosphate + gamma-L-Glu-L-beta-chloro-L-Ala
-
-
-
?
6.3.2.2
ATP + L-Glu + DL-allylglycine
-
1122
Rattus norvegicus
ADP + phosphate + gamma-L-Glu-DL-allylglycine
-
-
-
?
6.3.2.2
ATP + L-Glu + hydroxylamine
slow reaction rate
1122
Rattus norvegicus
ADP + phosphate + gamma-L-Glu-hydroxylamine
-
1122
Rattus norvegicus
?
6.3.2.2
ATP + L-Glu + L-2-aminobutanoate
-
1122
Rattus norvegicus
ADP + phosphate + gamma-L-Glu-2-aminobutanoate
-
-
-
?
6.3.2.2
ATP + L-Glu + L-Ala
-
1122
Rattus norvegicus
ADP + phosphate + gamma-L-Glu-L-Ala
-
-
-
?
6.3.2.2
ATP + L-Glu + L-Cys
-
1122
Rattus norvegicus
ADP + phosphate + gamma-L-Glu-L-Cys
-
-
-
?
6.3.2.2
ATP + L-Glu + L-Cys
glutathione biosynthesis
1122
Rattus norvegicus
?
-
-
-
?
6.3.2.2
ATP + L-Glu + L-homocysteine
-
1122
Rattus norvegicus
ADP + phosphate + gamma-L-Glu-L-homocysteine
-
-
-
?
6.3.2.2
ATP + L-Glu + L-norvaline
-
1122
Rattus norvegicus
ADP + phosphate + gamma-L-Glu-L-norvaline
-
-
-
?
6.3.2.2
ATP + L-Glu + L-Thr
-
1122
Rattus norvegicus
ADP + phosphate + gamma-L-Glu-L-Thr
-
-
-
?
6.3.2.2
ATP + N-methyl-L-glutarate + L-Cys
-
1122
Rattus norvegicus
ADP + phosphate + N-methyl-L-glutaryl-L-Cys
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.3.2.2
8
8.4
-
Rattus norvegicus
General Stability (protein specific)
EC Number
General Stability
Organism
6.3.2.2
treatment with 50 mM DTT for more than 1 h at 4°C, leads to some loss of activity, which is partially reversible. Further treatment leads to irreversible loss of activity
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
6.3.2.2
beta-Methylglutamate
-
Rattus norvegicus
6.3.2.2
buthionine sulfoximine
-
Rattus norvegicus
6.3.2.2
cystamine
completely reversible by DTT
Rattus norvegicus
6.3.2.2
D-3-amino-1-chloro-2-pentanone
highly potent irreversible inactivator
Rattus norvegicus
6.3.2.2
D-Glu
-
Rattus norvegicus
6.3.2.2
gamma-Methylglutamate
-
Rattus norvegicus
6.3.2.2
glutathione
-
Rattus norvegicus
6.3.2.2
L-2-Amino-4-oxo-5-chloropentanoate
inactivation requires very low concentration, 0.003-0.006 mM, of Mg2+ or certain other divalent cations, L-Glu, but not D-Glu protects competitively against inactivation, protection is increased in the presence of ATP or ADP
Rattus norvegicus
6.3.2.2
L-3-Amino-1-chloro-2-pentanone
highly potent irreversible inactivator
Rattus norvegicus
6.3.2.2
methionine sulfoximine
-
Rattus norvegicus
6.3.2.2
Prothionine sulfoximine
-
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.3.2.2
0.2
-
L-Cys
ATP, holoenzyme and recombinant heavy subunit
Rattus norvegicus
6.3.2.2
0.3
-
L-Cys
-
Rattus norvegicus
6.3.2.2
1
-
L-2-aminobutanoate
-
Rattus norvegicus
6.3.2.2
1.6
-
L-Glu
-
Rattus norvegicus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.3.2.2
104000
-
-
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
6.3.2.2
ATP + L-Glu + L-Cys
Rattus norvegicus
glutathione biosynthesis
?
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
6.3.2.2
-
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
6.3.2.2
kidney
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.3.2.2
additional information
-
-
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
6.3.2.2
ATP + alpha-methylglutamate + L-Cys
i.e. 2-amino-2-methylpentanedioate
1122
Rattus norvegicus
ADP + phosphate + alpha-methylglutamyl-L-Cys
-
-
-
?
6.3.2.2
ATP + beta-aminoglutarate + L-Cys
i.e. 3-aminopentanedioate
1122
Rattus norvegicus
ADP + phosphate + beta-aminoglutaryl-L-Cys
-
-
-
?
6.3.2.2
ATP + beta-methylglutamate + L-Cys
i.e. 2-amino-3-methylpentanedioate
1122
Rattus norvegicus
ADP + phosphate + beta-methylglutamyl-L-Cys
-
-
-
?
6.3.2.2
ATP + D-Glu + L-Cys
-
1122
Rattus norvegicus
ADP + phosphate + D-Glu-L-Cys
-
1122
Rattus norvegicus
?
6.3.2.2
ATP + L-Glu + beta-chloro-L-Ala
-
1122
Rattus norvegicus
ADP + phosphate + gamma-L-Glu-L-beta-chloro-L-Ala
-
-
-
?
6.3.2.2
ATP + L-Glu + DL-allylglycine
-
1122
Rattus norvegicus
ADP + phosphate + gamma-L-Glu-DL-allylglycine
-
-
-
?
6.3.2.2
ATP + L-Glu + hydroxylamine
slow reaction rate
1122
Rattus norvegicus
ADP + phosphate + gamma-L-Glu-hydroxylamine
-
1122
Rattus norvegicus
?
6.3.2.2
ATP + L-Glu + L-2-aminobutanoate
-
1122
Rattus norvegicus
ADP + phosphate + gamma-L-Glu-2-aminobutanoate
-
-
-
?
6.3.2.2
ATP + L-Glu + L-Ala
-
1122
Rattus norvegicus
ADP + phosphate + gamma-L-Glu-L-Ala
-
-
-
?
6.3.2.2
ATP + L-Glu + L-Cys
-
1122
Rattus norvegicus
ADP + phosphate + gamma-L-Glu-L-Cys
-
-
-
?
6.3.2.2
ATP + L-Glu + L-Cys
glutathione biosynthesis
1122
Rattus norvegicus
?
-
-
-
?
6.3.2.2
ATP + L-Glu + L-homocysteine
-
1122
Rattus norvegicus
ADP + phosphate + gamma-L-Glu-L-homocysteine
-
-
-
?
6.3.2.2
ATP + L-Glu + L-norvaline
-
1122
Rattus norvegicus
ADP + phosphate + gamma-L-Glu-L-norvaline
-
-
-
?
6.3.2.2
ATP + L-Glu + L-Thr
-
1122
Rattus norvegicus
ADP + phosphate + gamma-L-Glu-L-Thr
-
-
-
?
6.3.2.2
ATP + N-methyl-L-glutarate + L-Cys
-
1122
Rattus norvegicus
ADP + phosphate + N-methyl-L-glutaryl-L-Cys
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.3.2.2
8
8.4
-
Rattus norvegicus