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Literature summary extracted from

  • Watanabe, K.; Murata, K.; Kimura, A.
    Purification and characterization of gamma-glutamylcysteine synthetase of Escherichia coli B (1986), Agric. Biol. Chem., 50, 1925-1930.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.2.2 cystamine
-
Escherichia coli
6.3.2.2 cysteamine
-
Escherichia coli
6.3.2.2 glutathione feed-back inhibition; GSH inhibits, GSSG has no inhibitory effect Escherichia coli
6.3.2.2 Hg2+
-
Escherichia coli
6.3.2.2 iodoacetamide
-
Escherichia coli
6.3.2.2 NEM
-
Escherichia coli
6.3.2.2 PCMB
-
Escherichia coli
6.3.2.2 Thiocholine disulfide
-
Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.2 0.01
-
ATP
-
Escherichia coli
6.3.2.2 0.09
-
L-Cys
-
Escherichia coli
6.3.2.2 0.5
-
L-Glu
-
Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.2.2 Mg2+ divalent metal ion required, especially Mg2+, optimal concentration depending on ATP concentration Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.2.2 55000
-
gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.2 Escherichia coli
-
-
-
6.3.2.2 Escherichia coli B / ATCC 11303
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.2
-
Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.2.2 3.6
-
-
Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.2 ATP + L-Glu + D-Cys
-
Escherichia coli ADP + phosphate + gamma-L-Glu-D-Cys
-
?
6.3.2.2 ATP + L-Glu + D-Cys
-
Escherichia coli B / ATCC 11303 ADP + phosphate + gamma-L-Glu-D-Cys
-
?
6.3.2.2 ATP + L-Glu + L-2-aminobutanoate
-
Escherichia coli ADP + phosphate + gamma-L-Glu-2-aminobutanoate
-
?
6.3.2.2 ATP + L-Glu + L-2-aminobutanoate
-
Escherichia coli B / ATCC 11303 ADP + phosphate + gamma-L-Glu-2-aminobutanoate
-
?
6.3.2.2 ATP + L-Glu + L-Cys
-
Escherichia coli ADP + phosphate + gamma-L-Glu-L-Cys
-
?
6.3.2.2 ATP + L-Glu + L-Cys
-
Escherichia coli B / ATCC 11303 ADP + phosphate + gamma-L-Glu-L-Cys
-
?

Subunits

EC Number Subunits Comment Organism
6.3.2.2 monomer 1 * 55000, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.2.2 45
-
-
Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.3.2.2 55
-
pH 7.0, 35 min, 50% loss of activity Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.2.2 8.5
-
-
Escherichia coli

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
6.3.2.2 7
-
55°C, 35 min, 50% loss of activity Escherichia coli