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Literature summary extracted from

  • Nidetzky, B.; Furlinger, M.; Gollhofer, D.; Haug, I.; Haltrich, D.; Kulbe, K.D.
    Simultaneous enzymatic synthesis of gluconic acid and sorbitol. Production, purification, and application of glucose-fructose oxidoreductase and gluconolactonase (1997), Appl. Biochem. Biotechnol., 63-65, 173-188.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.99.28 Triton X-100 activates Zymomonas mobilis
1.1.99.28 Tween activates Zymomonas mobilis

Application

EC Number Application Comment Organism
3.1.1.17 analysis auxiliary enzyme for assaying glucose-fructose oxidoreductase activity Rhodotorula mucilaginosa

General Stability

EC Number General Stability Organism
1.1.99.28 inactivation during substrate conversion Zymomonas mobilis
1.1.99.28 thiol reagents stabilize. Dithiothreitol is the most efficient, 5-15 mM Zymomonas mobilis
3.1.1.17 100 mM NaCl, 4 mM K-gluconate or 25% glycerol slightly stabilize Rhodotorula mucilaginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.99.28 NAD+
-
 Zymomonas mobilis
1.1.99.28 NADP+
-
 Zymomonas mobilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.99.28 20
-
 glucose
-
 Zymomonas mobilis
1.1.99.28 400
-
 fructose
-
 Zymomonas mobilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.17 Mg2+ activates Rhodotorula mucilaginosa
3.1.1.17 Mn2+ activates Rhodotorula mucilaginosa

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.28 Zymomonas mobilis
-
 strain DSM 473
-
3.1.1.17 Delftia acidovorans
-
-
-
3.1.1.17 Pseudomonas aeruginosa
-
-
-
3.1.1.17 Pseudomonas chlororaphis
-
-
-
3.1.1.17 Pseudomonas fluorescens
-
-
-
3.1.1.17 Rhodotorula mucilaginosa
-
-
-
3.1.1.17 Zymomonas mobilis
-
 DSM 473
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.28
-
 Zymomonas mobilis
3.1.1.17
-
 Rhodotorula mucilaginosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.99.28 245 310
-
 Zymomonas mobilis
3.1.1.17 175.3
-
-
 Rhodotorula mucilaginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.28 2-Deoxy-D-glucose + D-fructose at 16% of the activity relative to D-glucose Zymomonas mobilis ?
-
 ?
1.1.99.28 D-glucose + D-fructose
-
 Zymomonas mobilis D-glucono-1,5-lactone + D-sorbitol
-
 ?
3.1.1.17 D-cellobiono-1,5-lactone + H2O
-
 Rhodotorula mucilaginosa ?
-
 ?
3.1.1.17 D-glucono-1,4-lactone + H2O no activity Rhodotorula mucilaginosa D-gluconate
-
 ?
3.1.1.17 D-glucono-1,5-lactone + H2O
-
 Pseudomonas fluorescens D-gluconate
-
 ?
3.1.1.17 D-glucono-1,5-lactone + H2O
-
 Pseudomonas aeruginosa D-gluconate
-
 ?
3.1.1.17 D-glucono-1,5-lactone + H2O
-
 Delftia acidovorans D-gluconate
-
 ?
3.1.1.17 D-glucono-1,5-lactone + H2O
-
 Zymomonas mobilis D-gluconate
-
 ?
3.1.1.17 D-glucono-1,5-lactone + H2O
-
 Rhodotorula mucilaginosa D-gluconate
-
 ?
3.1.1.17 D-glucono-1,5-lactone + H2O
-
 Pseudomonas chlororaphis D-gluconate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.99.28 40
-
-
 Zymomonas mobilis
3.1.1.17 32
-
-
 Rhodotorula mucilaginosa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.99.28 25
-
 stable for 2 weeks Zymomonas mobilis
1.1.99.28 45
-
 half-life: 55 h Zymomonas mobilis
3.1.1.17 4
-
 in presence of 0.1% Tween 20 and 5 mM dithiothreitol, half-life: 5 days Rhodotorula mucilaginosa
3.1.1.17 25
-
 in presence of 0.1% Tween 20 and 5 mM dithiothreitol, half-life: 35 days Rhodotorula mucilaginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.99.28 6.5 7
-
 Zymomonas mobilis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.99.28 5.5 6.8 30°C, pH does not affect the stability Zymomonas mobilis
1.1.99.28 7
-
 40°C, fast inactivation above Zymomonas mobilis