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Literature summary extracted from

  • Kataoka, M.; Shimizu, S.; Yamada, H.
    Purification and characterization of a novel FMN-dependent enzyme. Membrane-bound L-(+)-pantoyl lactone dehydrogenase from Nocardia asteroides (1992), Eur. J. Biochem., 204, 799-806.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.99.27 Brij 35 activates, maximal stimulation at 1.0% Nocardia asteroides

General Stability

EC Number General Stability Organism
1.1.99.27 Brij35 stabilizes Nocardia asteroides
1.1.99.27 some stabilization by glycerol and ethylene glycol Nocardia asteroides

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.99.27 (R)-pantolactone
-
 Nocardia asteroides
1.1.99.27 2-Dehydropantonolactone 20 mM Nocardia asteroides
1.1.99.27 5,5'-dithiobis(2-nitrobenzoate) 1 mM, 47% inhibition Nocardia asteroides
1.1.99.27 Atebrin 1 mM, 32.6% inhibition Nocardia asteroides
1.1.99.27 Barbital 1 mM, 54.5% inhibition Nocardia asteroides
1.1.99.27 CoCl2 1 mM, 24.4% inhibition Nocardia asteroides
1.1.99.27 Diphenylhydantoin 1 mM, 22.1% inhibition Nocardia asteroides
1.1.99.27 iodoacetate 1 mM, 17.4% inhibition Nocardia asteroides
1.1.99.27 MnCl2 1 mM, 86.2% inhibition Nocardia asteroides
1.1.99.27 NaAsO2 1 mM, 41% inhibition Nocardia asteroides
1.1.99.27 NaCN 1 mM, 51.9% inhibition Nocardia asteroides
1.1.99.27 NaF 1 mM, 27.3% inhibition Nocardia asteroides
1.1.99.27 NaN3 1 mM, 9.1% inhibition Nocardia asteroides
1.1.99.27 NEM 1 mM, 33.8% inhibition Nocardia asteroides
1.1.99.27 NH2OH*HCl 1 mM, 71.9% inhibition Nocardia asteroides
1.1.99.27 PCMB 1 mM, 41.0% inhibition Nocardia asteroides
1.1.99.27 riboflavin 1 mM, 74.9% inhibition Nocardia asteroides
1.1.99.27 Semicarbazide 1 mM, 23.1% inhibition Nocardia asteroides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.99.27 26.8
-
 (R)-pantolactone
-
 Nocardia asteroides

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.99.27 membrane bound Nocardia asteroides 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.99.27 42000
-
 x * 42000, SDS-PAGE of enzyme treated with 2-mercaptoethanol Nocardia asteroides
1.1.99.27 600000
-
 above 600000 Da, HPLC gel filtration Nocardia asteroides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.99.27 additional information Nocardia asteroides 1,2-propanediol induces the enzyme ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.27 Nocardia asteroides
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.1.99.27 no glycoprotein
-
 Nocardia asteroides

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.27
-
 Nocardia asteroides

Storage Stability

EC Number Storage Stability Organism
1.1.99.27 4°C, pH 7.4, 0.1 U/ml enzyme, 0.01 M Tris-HCl, 0.1 mM dithiothreitol, 0.5% Brij35, 2.9% loss of activity after 2 weeks Nocardia asteroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.27 (R)-Pantolactone + (Fe(CN)6)3-
-
 Nocardia asteroides 2-Dehydropantonolactone + Fe(CN)64-
-
 ?
1.1.99.27 (R)-Pantolactone + 2,6-dichlorophenol-indophenol
-
 Nocardia asteroides 2-Dehydropantonolactone + reduced 2,6-dichlorophenol-indophenol
-
 ?
1.1.99.27 (R)-Pantolactone + indonitrotetrazolium
-
 Nocardia asteroides 2-Dehydropantonolactone + reduced indonitrotetrazolium
-
 ?
1.1.99.27 (R)-Pantolactone + medola blue
-
 Nocardia asteroides 2-Dehydropantonolactone + reduced medola blue
-
 ?
1.1.99.27 (R)-Pantolactone + nitrotetrazolium blue
-
 Nocardia asteroides 2-Dehydropantonolactone + reduced nitrotetrazolium blue
-
 ?
1.1.99.27 (R)-Pantolactone + phenazine methosulfate highly specific for (R)-pantolactone Nocardia asteroides 2-Dehydropantonolactone + reduced phenazine methosulfate
-
 ?
1.1.99.27 additional information 1,2-propanediol induces the enzyme Nocardia asteroides ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.99.27 ? x * 42000, SDS-PAGE of enzyme treated with 2-mercaptoethanol Nocardia asteroides

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.99.27 40
-
-
 Nocardia asteroides

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.99.27 25 45 about 20% of maximal activity at 25°C and 45°C Nocardia asteroides

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.99.27 30
-
 pH 9.0-10.0, 30 min, stable Nocardia asteroides
1.1.99.27 35
-
 pH 7.0, 10 min, stable below Nocardia asteroides
1.1.99.27 45
-
 pH 7.0, 10 min, 43.1% loss of activity Nocardia asteroides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.99.27 9 9.5
-
 Nocardia asteroides

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.99.27 FMN dependent on, noncovalently bound Nocardia asteroides