Literature summary extracted from

  • Atkinson, I.; Garrow, T.; Brenner, A.; Shane, B.
    Human cytosolic folylpoly-gamma-glutamate synthase (1997), Methods Enzymol., 281, 134-140.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.3.2.17 Reducing agents absolutely dependent on Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.17 expression in Escherichia coli Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.17 0.0014
-
5,6,7,8-tetrahydropteroyl-Glu3
-
Homo sapiens
6.3.2.17 0.012
-
7,8-dihydropteroyl-(4-aminobutanoyl)-Glu pteroyl-Glu4 Homo sapiens
6.3.2.17 0.0033
-
5,6,7,8-tetrahydropteroyl-Glu2
-
Homo sapiens
6.3.2.17 0.013
-
5-formyl-5,6,7,8-tetrahydropteroyl-Glu2
-
Homo sapiens
6.3.2.17 0.02
-
methotrexate-Glu3 pteroyl-Glu3 Homo sapiens
6.3.2.17 0.2
-
L-Glu MgATP2- Homo sapiens
6.3.2.17 0.0016
-
5,6,7,8-tetrahydropteroyl-Glu4
-
Homo sapiens
6.3.2.17 0.0037
-
10-formyl-5,6,7,8-tetrahydropteroyl-Glu
-
Homo sapiens
6.3.2.17 0.048
-
7,8-dihydropteroyl-Glu2
-
Homo sapiens
6.3.2.17 0.016
-
pteroyl-Glu2
-
Homo sapiens
6.3.2.17 0.105
-
5-formyl-5,6,7,8-tetrahydropteroyl-Glu
-
Homo sapiens
6.3.2.17 0.059
-
pteroyl-Glu
-
Homo sapiens
6.3.2.17 0.00081
-
7,8-dihydropteroyl-Glu
-
Homo sapiens
6.3.2.17 0.0027
-
5,6,7,8-tetrahydropteroyl-Glu5 10-formyl-5,6,7,8-tetrahydropteroyl-Glu2 Homo sapiens
6.3.2.17 0.0044
-
5,6,7,8-tetrahydropteroyl-Glu aminopterin Homo sapiens
6.3.2.17 0.064
-
pteroyl-Glu5
-
Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.3.2.17 cytosol
-
Homo sapiens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.2.17 K+ maximal activity at 20 mM Homo sapiens
6.3.2.17 K+ required form maximal activity Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.2.17 61000
-
gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.17 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.17
-
Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.2.17 0.31
-
-
Homo sapiens

Storage Stability

EC Number Storage Stability Organism
6.3.2.17 0-4°C, stable for several days, or at -20°C for longer periods Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.17 ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu
-
Homo sapiens ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
?
6.3.2.17 ATP + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
-
Homo sapiens ADP + phosphate + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glu2
-
?
6.3.2.17 ATP + pteroyl-Glu + Glu
-
Homo sapiens ADP + phosphate + pteroylmonoglutamyl-Glu
-
?
6.3.2.17 ATP + (6S)-5,6,7,8-tetrahydropteroylglutamate + L-glutamate
-
Homo sapiens ADP + phosphate + (6S)-5,6,7,8-tetrahydropteroyl-Glu2
-
?
6.3.2.17 ADP + phosphate + 7,8-dihydropteroyl-Glu
-
Homo sapiens ATP + 7,8-dihydropteroate + L-glutamate
-
?
6.3.2.17 ATP + pteroyl-Glu3 + L-glutamate
-
Homo sapiens ADP + phosphate + pteroyl-Glu4
-
?
6.3.2.17 ATP + 5,6,7,8-tetrahydropteroyl-Glu3 + L-glutamate
-
Homo sapiens ADP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu4
-
?
6.3.2.17 ATP + 5,6,7,8-tetrahydropteroyl-Glu5 + L-glutamate
-
Homo sapiens ADP + phosphate + 5,6,7,8-tetrahydropteroyl-Glu6
-
?
6.3.2.17 ATP + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu + L-glutamate
-
Homo sapiens ADP + phosphate + 10-formyl-5,6,7,8-tetrahydropteroyl-Glu2
-
?
6.3.2.17 ATP + 5-formyl-5,6,7,8-tetrahydropteroyl-Glu + L-glutamate
-
Homo sapiens ADP + phosphate + 5-formyl-5,6,7,8-tetrahydropteroyl-Glu2
-
?
6.3.2.17 ATP + 7,8-dihydropteroyl-(4-aminobutanoyl)-Glu + L-glutamate
-
Homo sapiens ADP + phosphate + 7,8-dihydropteroyl-(4-aminobutanoyl)-Glu2
-
?
6.3.2.17 ATP + 7,8-dihydropteroyl-Glu + L-glutamate
-
Homo sapiens ADP + phosphate + 7,8-dihydropteroyl-Glu2
-
?
6.3.2.17 ATP + methotrexate + L-glutamate
-
Homo sapiens ADP + phosphate + methotrexyl-Glu
-
?
6.3.2.17 ATP + pteroyl-Glu5 + L-glutamate
-
Homo sapiens ADP + phosphate + pteroyl-Glu6
-
?
6.3.2.17 ATP + pteroyl-Glu4 + L-glutamate
-
Homo sapiens ADP + phosphate + pteroyl-Glu5
-
?

Subunits

EC Number Subunits Comment Organism
6.3.2.17 monomer 1 * 61000, SDS-PAGE Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.2.17 additional information
-
additional information
-
Homo sapiens
6.3.2.17 0.55
-
pteroyl-Glu3
-
Homo sapiens
6.3.2.17 0.99
-
5,6,7,8-tetrahydropteroyl-Glu
-
Homo sapiens
6.3.2.17 0.01
-
pteroyl-Glu5
-
Homo sapiens
6.3.2.17 0.34
-
pteroyl-Glu4
-
Homo sapiens
6.3.2.17 0.65
-
pteroyl-Glu
-
Homo sapiens
6.3.2.17 0.81
-
5,6,7,8-tetrahydropteroyl-Glu2
-
Homo sapiens
6.3.2.17 0.82
-
pteroyl-Glu2
-
Homo sapiens
6.3.2.17 0.84
-
7,8-dihydropteroyl-Glu
-
Homo sapiens
6.3.2.17 0.95
-
7,8-dihydropteroyl-Glu2
-
Homo sapiens
6.3.2.17 1.17
-
aminopterin
-
Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.2.17 9.6
-
-
Homo sapiens