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Literature summary extracted from

  • Pristupa, Z.B.; Vickers, P.J.; Sephton, G.B.; Scrimgeour, K.G.
    Folylpolyglutamate synthetase from beef liver: assay, stabilization, and characterization (1984), Can. J. Biochem. Cell Biol., 62, 495-506.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.3.2.17 2-mercaptoethanol maximal activity at 30-50 mM Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.17 0.009
-
5,6,7,8-tetrahydropteroyl-Glu
-
Bos taurus
6.3.2.17 0.01
-
ATP 5-formyl-5,6,7,8-tetrahydropteroyl-Glu Bos taurus
6.3.2.17 0.025
-
aminopterin ATP Bos taurus
6.3.2.17 0.09
-
methotrexate
-
Bos taurus
6.3.2.17 0.1
-
methotrexate aminopterin Bos taurus
6.3.2.17 0.82
-
L-Glu
-
Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.2.17 K+ maximal activity at 10-20 mM Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.17 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.17 partial Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.2.17 liver
-
Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.2.17 additional information
-
-
Bos taurus

Storage Stability

EC Number Storage Stability Organism
6.3.2.17 -20°C, stable for 3 weeks Bos taurus
6.3.2.17 0°C, in phosphate-mercaptoethanol, pH 7.5, 75% of activity is retained after 10 days, 55% after 3 weeks Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.17 ATP + 5,6,7,8-tetrahydropteroyl-Glu + L-glutamate
-
Bos taurus ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glu2
-
?
6.3.2.17 ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu
-
Bos taurus ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
?
6.3.2.17 ATP + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
-
Bos taurus ADP + phosphate + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glu2
-
?
6.3.2.17 ATP + aminopterin + Glu most active substrate Bos taurus ADP + phosphate + aminopteryl-Glu
-
?
6.3.2.17 ATP + methotrexate + Glu
-
Bos taurus ADP + phosphate + methotrexyl-Glu
-
?
6.3.2.17 ATP + pteroyl-Glu + Glu
-
Bos taurus ADP + phosphate + pteroylmonoglutamyl-Glu
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.2.17 8.4
-
glutamylation of methotrexate Bos taurus