EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
6.3.2.17 | 2-mercaptoethanol | maximal activity at 30-50 mM | Bos taurus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.2.17 | 0.009 | - |
5,6,7,8-tetrahydropteroyl-Glu | - |
Bos taurus | |
6.3.2.17 | 0.01 | - |
ATP | 5-formyl-5,6,7,8-tetrahydropteroyl-Glu | Bos taurus | |
6.3.2.17 | 0.025 | - |
aminopterin | ATP | Bos taurus | |
6.3.2.17 | 0.09 | - |
methotrexate | - |
Bos taurus | |
6.3.2.17 | 0.1 | - |
methotrexate | aminopterin | Bos taurus | |
6.3.2.17 | 0.82 | - |
L-Glu | - |
Bos taurus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
6.3.2.17 | K+ | maximal activity at 10-20 mM | Bos taurus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.3.2.17 | Bos taurus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.3.2.17 | partial | Bos taurus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
6.3.2.17 | liver | - |
Bos taurus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
6.3.2.17 | additional information | - |
- |
Bos taurus |
EC Number | Storage Stability | Organism |
---|---|---|
6.3.2.17 | -20°C, stable for 3 weeks | Bos taurus |
6.3.2.17 | 0°C, in phosphate-mercaptoethanol, pH 7.5, 75% of activity is retained after 10 days, 55% after 3 weeks | Bos taurus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.2.17 | ATP + 5,6,7,8-tetrahydropteroyl-Glu + L-glutamate | - |
Bos taurus | ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glu2 | - |
? | |
6.3.2.17 | ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu | - |
Bos taurus | ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1 | - |
? | |
6.3.2.17 | ATP + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu | - |
Bos taurus | ADP + phosphate + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glu2 | - |
? | |
6.3.2.17 | ATP + aminopterin + Glu | most active substrate | Bos taurus | ADP + phosphate + aminopteryl-Glu | - |
? | |
6.3.2.17 | ATP + methotrexate + Glu | - |
Bos taurus | ADP + phosphate + methotrexyl-Glu | - |
? | |
6.3.2.17 | ATP + pteroyl-Glu + Glu | - |
Bos taurus | ADP + phosphate + pteroylmonoglutamyl-Glu | - |
? |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
6.3.2.17 | 8.4 | - |
glutamylation of methotrexate | Bos taurus |