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Literature summary extracted from

  • Fraaije, M.W.; van der Heuvel, R.H.H.; Roelofs, J.C.A.A.; van Berkel, W.J.H.
    Kinetic mechanism of vanillyl-alcohol oxidase with short-chain 4-alkylphenols (1998), Eur. J. Biochem., 253, 712-719.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.38 0.0037
-
 4-Propylphenol
-
 Penicillium simplicissimum
1.1.3.38 0.009
-
 4-Ethylphenol
-
 Penicillium simplicissimum
1.1.3.38 0.031
-
 4-Methylphenol
-
 Penicillium simplicissimum

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.38 Penicillium simplicissimum
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.38 4-ethylphenol + O2 transient intermediate: p-quinone methide of the aromatic substrate in complex with reduced enzyme. Ordered sequential binding mechanism in which the rate of flavin reduction determines the turnover rate while the reduced enzyme-p-quinone methide binary complex rapidly reacts with dioxygen Penicillium simplicissimum 1-(4'-hydroxyphenyl)ethanol + 4-vinylphenol + 4-hydroxyacetophenone
-
 ?
1.1.3.38 4-Methylphenol + O2 transient intermediate: p-quinone methide of the aromatic substrate in complex with reduced enzyme. Ordered sequential binding mechanism in which the rate of flavin reduction determines the turnover rate while the reduced enzyme-p-quinone methide binary complex rapidly reacts with dioxygen Penicillium simplicissimum ?
-
 ?
1.1.3.38 4-Propylphenol + O2
-
 Penicillium simplicissimum 1-(4'-Hydroxyphenyl)propanol + 4-propenylphenol + 1-(4'-hydroxyphenyl)propanone
-
 ?
1.1.3.38 propylphenol + O2
-
 Penicillium simplicissimum ?
-
 r

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.3.38 0.005
-
 4-Methylphenol
-
 Penicillium simplicissimum
1.1.3.38 2.5
-
 4-Ethylphenol
-
 Penicillium simplicissimum
1.1.3.38 4.2
-
 4-n-Propylphenol
-
 Penicillium simplicissimum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.38 flavin a covalent 5-(4'-hydroxybenzyl)-FAD adduct is formed during reaction with 4-methylphenol Penicillium simplicissimum