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Literature summary extracted from

  • Michaud, C.; Mengin-Lecreulx, D.; van Heijenoort, J.; Blanot, D.
    Over-production, purification and properties of the uridine-diphosphate-N-acetylmuramoyl-L-alanyl-D-glutamate:meso-2,6-diaminopimelate ligase from Escherichia coli (1990), Eur. J. Biochem., 194, 853-861.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.2.13 additional information
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.13 0.036
-
 meso-2,6-diaminoheptanedioate
-
 Escherichia coli
6.3.2.13 0.076
-
 UDP-N-MurNAc-L-Ala-D-Glu
-
 Escherichia coli
6.3.2.13 0.62
-
 ATP
-
 Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.3.2.13 cytoplasm
-
 Escherichia coli 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.2.13 K+ potassium phosphate increases activity Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.2.13 56000
-
 1 * 56000, SDS-PAGE Escherichia coli
6.3.2.13 62000
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.13 Escherichia coli
-
 overproduced from strain JM83(pHE5) and the very strong overproducing strain KM83(pMLD25)
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.13
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.2.13 0.0393
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
6.3.2.13 -20°C, complete loss of activity after 2 d Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.13 ATP + UDP-MurNAc-L-Ala-D-Glu + meso-2,6-diaminoheptanedioate r Escherichia coli ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.2.13 monomer 1 * 56000, SDS-PAGE Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.2.13 8
-
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.3.2.13 7.2 9.5 about 40% of maximal activity at pH 7.2 and 9.5 Escherichia coli