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Literature summary extracted from

  • Ikeda, M.; Iwai, K.
    Purification and properties of the dihydrofolate synthetase from Serratia indica (1976), J. Nutr. Sci. Vitaminol., 22, 235-248.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.2.12 ADP
-
 Serratia indica
6.3.2.12 PCMB
-
 Serratia indica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.2.12 K+ required Serratia indica
6.3.2.12 Mg2+ required Serratia indica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.2.12 47000
-
 gel filtration Serratia indica

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.12 Serratia indica
-
 IFO 3759
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.12
-
 Serratia indica

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.2.12 0.0155
-
-
 Serratia indica

Storage Stability

EC Number Storage Stability Organism
6.3.2.12 -20°C, unstable Serratia indica
6.3.2.12 stable in the form of an ammonium sulfate precipitate in an ice bath for 3 months Serratia indica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.12 ATP + 7,8-dihydropteroate + L-Glu
-
 Serratia indica ADP + phosphate + 7,8-dihydrofolate
-
 ?
6.3.2.12 GTP + 7,8-dihydropteroate + L-Glu 37% of the activity relative to ATP Serratia indica GDP + phosphate + 7,8-dihydrofolate
-
 ?
6.3.2.12 ITP + 7,8-dihydropteroate + L-Glu 59% of the activity relative to ATP Serratia indica IDP + phosphate + 7,8-dihydrofolate
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.3.2.12 70
-
 30 min, almost complete loss of activity Serratia indica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.2.12 9
-
-
 Serratia indica

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.3.2.12 7.5 11 7.5: about 30% of maximal activity, 11.0: about 70% of maximal activity Serratia indica