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Literature summary extracted from

  • Roland, S.; Ferone, R.; Harvey, R.J.; Styles, V.L.; Morrison, R.W.
    The characteristics and significance of sulfonamides as substrates for Escherichia coli dihydropteroate synthase (1979), J. Biol. Chem., 254, 10337-10345.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.15 sulfamethoxazole competitive to 4-aminobenzoate Escherichia coli
2.5.1.15 sulfanilamide
-
 Escherichia coli
2.5.1.15 sulfathiazole competitive to 4-aminobenzoate Escherichia coli
2.5.1.15 sulfonamide competitive to 4-aminobenzoate Escherichia coli
6.3.2.12 Dihydropterin-sulfonamides
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.15 0.00012
-
 sulfamethoxazole
-
 Escherichia coli
2.5.1.15 0.00057
-
 4-Aminobenzoate
-
 Escherichia coli
2.5.1.15 0.001
-
 sulfathiazole
-
 Escherichia coli
2.5.1.15 0.0048
-
 sulfanilamide
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.15 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + 4-aminobenzoate Escherichia coli
-
 diphosphate + 7,8-dihydropteroate
-
 ?
2.5.1.15 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + 4-aminobenzoate Escherichia coli B / ATCC 11303
-
 diphosphate + 7,8-dihydropteroate
-
 ?
2.5.1.15 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + sulfamethoxazole Escherichia coli
-
 diphosphate + dihydropterin-sulfamethoxazole
-
 ?
2.5.1.15 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + sulfamethoxazole Escherichia coli B / ATCC 11303
-
 diphosphate + dihydropterin-sulfamethoxazole
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.15 Escherichia coli
-
-
-
6.3.2.12 Escherichia coli
-
 B
-
6.3.2.12 Escherichia coli B / ATCC 11303
-
 B
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.15 gel filtration on Sephadex G-100 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.15 1 2
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.15 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + 4-aminobenzoate
-
 Escherichia coli diphosphate + 7,8-dihydropteroate
-
 ?
2.5.1.15 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + 4-aminobenzoate
-
 Escherichia coli B / ATCC 11303 diphosphate + 7,8-dihydropteroate
-
 ?
2.5.1.15 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + sulfamethoxazole
-
 Escherichia coli diphosphate + dihydropterin-sulfamethoxazole
-
 ?
2.5.1.15 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + sulfamethoxazole
-
 Escherichia coli B / ATCC 11303 diphosphate + dihydropterin-sulfamethoxazole
-
 ?
2.5.1.15 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + sulfanilamide
-
 Escherichia coli diphosphate + dihydropterin-sulfanilamide
-
 ?
2.5.1.15 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + sulfathiazole
-
 Escherichia coli diphosphate + dihydropterin-sulfathiazole
-
 ?
2.5.1.15 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + sulfathiazole
-
 Escherichia coli B / ATCC 11303 diphosphate + dihydropterin-sulfathiazole
-
 ?
6.3.2.12 ATP + 7,8-dihydropteroate + L-Glu
-
 Escherichia coli ADP + phosphate + 7,8-dihydrofolate
-
 ?
6.3.2.12 ATP + 7,8-dihydropteroate + L-Glu
-
 Escherichia coli B / ATCC 11303 ADP + phosphate + 7,8-dihydrofolate
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.15 0.00013
-
 sulfamethoxazole
-
 Escherichia coli
2.5.1.15 0.00053
-
 sulfonamide
-
 Escherichia coli
2.5.1.15 0.0057
-
 sulfanilamide
-
 Escherichia coli